Functional analysis of centrosomal kinase substrates in Drosophila melanogaster reveals a new function of the nuclear envelope component otefin in cell cycle progression.

abstract：:Sporulation in Saccharomyces cerevisiae normally occurs only in MATa/MAT alpha diploids. We show that mutations in RME1 bypassed the requirements for both a and alpha mating type information in sporulation and therefore allowed MATa/MATa and MAT alpha/MAT alpha diploids to sporulate. RME1 was located on chromosome VII...

journal_title：Molecular and cellular biology

authors： Rine J,Sprague GF Jr,Herskowitz I

更新日期：1981-10-01 00:00:00

abstract：:NF-κB activation is a critical component in the transcriptional response to hypoxia. However, the underlying mechanisms that control its activity under these conditions are unknown. Here we report that under hypoxic conditions, IκB kinase (IKK) activity is induced through a calcium/calmodulin-dependent kinase 2 (CaMK2...

journal_title：Molecular and cellular biology

authors： Culver C,Sundqvist A,Mudie S,Melvin A,Xirodimas D,Rocha S

更新日期：2010-10-01 00:00:00

abstract：:Pseudo-exons are intronic sequences that are flanked by apparent consensus splice sites but that are not observed in spliced mRNAs. Pseudo-exons are often difficult to activate by mutation and have typically been viewed as a conceptual challenge to our understanding of how the spliceosome discriminates between authent...

journal_title：Molecular and cellular biology

authors： Grellscheid SN,Smith CW

更新日期：2006-03-01 00:00:00

abstract：:Src homology 2 (SH2) domains provide specificity to intracellular signaling by binding to specific phosphotyrosine (phospho-Tyr)-containing sequences. We recently developed a technique using a degenerate phosphopeptide library to predict the specificity of individual SH2 domains (src family members, Abl, Nck, Sem5, ph...

journal_title：Molecular and cellular biology

authors： Songyang Z,Shoelson SE,McGlade J,Olivier P,Pawson T,Bustelo XR,Barbacid M,Sabe H,Hanafusa H,Yi T

更新日期：1994-04-01 00:00:00

abstract：:RNA interference with one of the eight Caenorhabditis elegans linker histone genes triggers desilencing of a repetitive transgene and developmental defects in the hermaphrodite germ line. These characteristics are similar to the phenotype of the C. elegans Polycomb group genes mes-2, mes-3, mes-4, and mes-6 (M. A. Jed...

journal_title：Molecular and cellular biology

authors： Jedrusik MA,Schulze E

更新日期：2007-03-01 00:00:00

abstract：:The enhancer of yellow 1 gene, e(y)1, of Drosophila melanogaster has been cloned and demonstrated to encode the TAFII40 protein. The e(y)1 gene is expressed in females much more strongly than in males due to the accumulation of e(y)1 mRNA in the ovaries. Two different e(y)1 mutations have been obtained. The e(y)1(ul) ...

journal_title：Molecular and cellular biology

authors： Soldatov A,Nabirochkina E,Georgieva S,Belenkaja T,Georgiev P

更新日期：1999-05-01 00:00:00

abstract：:The p53 tumor suppressor gene product is a sequence-specific DNA-binding protein that is necessary for the G1 arrest of many cell types. Consistent with its role as a cell cycle checkpoint factor, p53 has been shown to be capable of both transcriptional activation and repression. Here we show a new potential role for ...

journal_title：Molecular and cellular biology

authors： Miller SD,Farmer G,Prives C

更新日期：1995-12-01 00:00:00

abstract：:Osteoclast differentiation factor (ODF)/receptor activator of NF-kappaB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the CCAAT box on the ODF promoter and regulates its basal transcriptional activity. The CCAAT box on the ODF gene is required...

journal_title：Molecular and cellular biology

authors： Kabe Y,Yamada J,Uga H,Yamaguchi Y,Wada T,Handa H

更新日期：2005-01-01 00:00:00

abstract：:In heat-shocked tomato cell cultures, cytoplasmic heat shock granules (HSGs) are tightly associated with a specific subset of mRNAs coding mainly for the untranslated control proteins. This messenger ribonucleoprotein complex was banded in a CsCl gradient after fixation with formaldehyde (approximately 1.30 g/cm3). It...

journal_title：Molecular and cellular biology

authors： Nover L,Scharf KD,Neumann D

更新日期：1989-03-01 00:00:00

abstract：:Alpha interferon (IFN-alpha) and IFN-beta are able to interfere with viral infection. They exert a vast array of biologic functions, including growth arrest, cell differentiation, and immune system regulation. This regulation extends from innate immunity to cellular and humoral adaptive immune responses. A strict cont...

journal_title：Molecular and cellular biology

authors： Mesplède T,Island ML,Christeff N,Petek F,Doly J,Navarro S

更新日期：2005-10-01 00:00:00

abstract：:The expression of the Drosophila segmentation gene fushi tarazu (ftz) is controlled at the level of transcription. The proximal enhancer, located approximately 3.4 kb upstream of the transcription start site, directs lacZ fusion gene expression in a ftz-like seven-stripe pattern in transgenic fly embryos. We have take...

journal_title：Molecular and cellular biology

authors： Han W,Yu Y,Altan N,Pick L

更新日期：1993-09-01 00:00:00

abstract：:Xenopus laevis oogenesis is characterized by an active transcription which ceases abruptly upon maturation. To survey changes in the characteristics of the transcriptional machinery which might contribute to this transcriptional arrest, the phosphorylation status of the RNA polymerase II largest subunit (RPB1 subunit)...

journal_title：Molecular and cellular biology

authors： Bellier S,Dubois MF,Nishida E,Almouzni G,Bensaude O

更新日期：1997-03-01 00:00:00

abstract：:Translation is a fundamental step in gene expression, and translational control is exerted in many developmental processes. Most eukaryotic mRNAs are translated by a cap-dependent mechanism, which requires recognition of the 5'-cap structure of the mRNA by eukaryotic translation initiation factor 4E (eIF4E). eIF4E act...

journal_title：Molecular and cellular biology

authors： Hernández G,Miron M,Han H,Liu N,Magescas J,Tettweiler G,Frank F,Siddiqui N,Sonenberg N,Lasko P

更新日期：2013-08-01 00:00:00

abstract：:B-myc is a recently described myc gene whose product has not been functionally characterized. The predicted product of B-myc is a 168-amino-acid protein with extensive homology to the c-Myc amino-terminal region, previously shown to contain a transcriptional activation domain. We hypothesized that B-Myc might also fun...

journal_title：Molecular and cellular biology

authors： Resar LM,Dolde C,Barrett JF,Dang CV

更新日期：1993-02-01 00:00:00

abstract：:Mammalian Ras GTPase-activating protein (GAP), p120 Ras-GAP, has been implicated as both a downregulator and effector of Ras proteins, but its precise role in Ras-mediated signal transduction pathways is unclear. To begin a genetic analysis of the role of p120 Ras-GAP we identified a homolog from the fruit fly Drosoph...

journal_title：Molecular and cellular biology

authors： Feldmann P,Eicher EN,Leevers SJ,Hafen E,Hughes DA

更新日期：1999-03-01 00:00:00

abstract：:The Caenorhabditis elegans run gene encodes a Runt domain factor. Runx1, Runx2, and Runx3 are the three known mammalian homologs of run. Runx1, which plays an essential role in hematopoiesis, has been identified at the breakpoint of chromosome translocations that are responsible for human leukemia. Runx2 plays an esse...

journal_title：Molecular and cellular biology

authors： Nam S,Jin YH,Li QL,Lee KY,Jeong GB,Ito Y,Lee J,Bae SC

更新日期：2002-01-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the CDC25 gene product activates adenylate cyclase through RAS1 and RAS2 gene products. We have recently described the cloning of a DNA fragment which suppresses the cdc25 mutation but not ras1, ras2, or cdc35 mutations. This fragment contains a 5'-truncated open reading frame wh...

journal_title：Molecular and cellular biology

authors： Damak F,Boy-Marcotte E,Le-Roscouet D,Guilbaud R,Jacquet M

更新日期：1991-01-01 00:00:00

abstract：:Box C/D snoRNPs, factors essential for ribosome biogenesis, are proposed to be assembled in the nucleoplasm before localizing to the nucleolus. However, recent work demonstrated the involvement of nuclear export factors in this process, suggesting that export may take place. Here we show that there are distinct distri...

journal_title：Molecular and cellular biology

authors： Watkins NJ,Lemm I,Lührmann R

更新日期：2007-10-01 00:00:00

abstract：:T-cell activation requires two different signals. The T-cell receptor's recognition of a specific antigen on antigen-presenting cells provides one, and the second signal comes from costimulatory molecules such as CD28. In contrast, T cells that are stimulated with antigen in the absence of the CD28 costimulatory signa...

journal_title：Molecular and cellular biology

authors： Lai JH,Horvath G,Subleski J,Bruder J,Ghosh P,Tan TH

更新日期：1995-08-01 00:00:00

abstract：:By injecting the appropriately constructed plasmids into one-cell Xenopus embryos, we determined that the 3' region of the maternal Xenopus Eg2 mRNA confers instability on the chimeric mRNA transcribed from these plasmids. This instability, like that of the maternal Eg2 transcript, was abolished by treatment of the em...

journal_title：Molecular and cellular biology

authors： Bouvet P,Paris J,Phillippe M,Osborne HB

更新日期：1991-06-01 00:00:00

abstract：:CD8+ T-cell apoptosis is essential for the contraction phase of the immune response, yet the initiating signals and precise pathways involved are unresolved. The ST3Gal-I sialyltransferase is a candidate mechanistic component and catalyzes sialic acid addition to core 1 O-glycans during protein O glycosylation. ST3Gal...

journal_title：Molecular and cellular biology

authors： Van Dyken SJ,Green RS,Marth JD

更新日期：2007-02-01 00:00:00

abstract：:The mechanism for transcriptional silencing of pericentric heterochromatin is conserved from fission yeast to mammals. Silenced genome regions are marked by epigenetic methylation of histone H3, which serves as a binding site for structural heterochromatin proteins. In the fission yeast Schizosaccharomyces pombe, the ...

journal_title：Molecular and cellular biology

authors： Cheutin T,Gorski SA,May KM,Singh PB,Misteli T

更新日期：2004-04-01 00:00:00

abstract：:Protein acetylation has emerged as a means of controlling levels of mRNA synthesis in eukaryotic cells. Here we report that acetyl coenzyme A (acetyl-CoA) stimulates RNA polymerase II transcription in vitro in the absence of histones. The effect of acetyl-CoA on basal and activated transcription was studied in a human...

journal_title：Molecular and cellular biology

authors： Galasinski SK,Lively TN,Grebe De Barron A,Goodrich JA

更新日期：2000-03-01 00:00:00

abstract：:Mitotic chromosome condensation is chiefly driven by the condensin complex. The specific recognition (targeting) of chromosomal sites by condensin is an important component of its in vivo activity. We previously identified the rRNA gene cluster in Saccharomyces cerevisiae as an important condensin-binding site, but bo...

journal_title：Molecular and cellular biology

authors： Wang BD,Eyre D,Basrai M,Lichten M,Strunnikov A

更新日期：2005-08-01 00:00:00

abstract：:The Surf-3 gene of the unusually tight mouse Surfeit locus gene cluster has been identified as the highly conserved ribosomal protein gene L7a (rpL7a). The topography and juxtaposition of the Surfeit locus genes are conserved for the 600 million years of divergent evolution between mammals and birds. This suggests cis...

journal_title：Molecular and cellular biology

authors： Armes N,Fried M

更新日期：1995-05-01 00:00:00

abstract：:GCN4 is a transcriptional activator of amino acid-biosynthetic genes in the yeast Saccharomyces cerevisiae. GCN2, a translational activator of GCN4 expression, contains a domain homologous to the catalytic subunit of eucaryotic protein kinases. Substitution of a highly conserved lysine residue in the kinase domain abo...

journal_title：Molecular and cellular biology

authors： Wek RC,Ramirez M,Jackson BM,Hinnebusch AG

更新日期：1990-06-01 00:00:00

abstract：:Thyroid hormone receptor (TR) functions as part of multiprotein complexes that also include retinoid X receptor (RXR) and transcriptional coregulators. We have found that both the TR CoR box and ninth heptad are required for RXR interaction and in turn for interaction with corepressor proteins N-CoR and SMRT. Remarkab...

journal_title：Molecular and cellular biology

authors： Zhang J,Zamir I,Lazar MA

更新日期：1997-12-01 00:00:00

abstract：:Metazoan replication-dependent histone mRNAs are the only eukaryotic mRNAs that are not polyadenylated. The cleavage of histone pre-mRNA to form the unique 3' end requires the U7 snRNP and the stem-loop binding protein (SLBP) that binds the 3' end of histone mRNA. U7 snRNP contains three novel proteins, Lsm10 and Lsm1...

journal_title：Molecular and cellular biology

authors： Wagner EJ,Marzluff WF

更新日期：2006-09-01 00:00:00

abstract：:In yeast cells, mutations in the TATA-binding protein (TBP) that disrupt the interaction with the TATA element or with TFIIA can selectively impair the response to acidic activator proteins. We analyzed the transcriptional properties of TBP derivatives in which residues that directly interact with TFIIB were replaced ...

journal_title：Molecular and cellular biology

authors： Lee M,Struhl K

更新日期：1997-03-01 00:00:00

abstract：:Members of the transforming growth factor beta (TGF-beta) family transduce signals through Smad proteins. Smad signaling can be regulated by the Ras/Erk/mitogen-activated protein pathway in response to receptor tyrosine kinase activation and the gamma interferon pathway and also by the functional interaction of Smad2 ...

journal_title：Molecular and cellular biology

authors： Wicks SJ,Lui S,Abdel-Wahab N,Mason RM,Chantry A

更新日期：2000-11-01 00:00:00