The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter.

abstract：:Human 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II (RNAPII) in vitro. However, the physiological roles of this negative regulation are not well understood. Here, by using a num...

journal_title：Molecular and cellular biology

authors： Aida M,Chen Y,Nakajima K,Yamaguchi Y,Wada T,Handa H

更新日期：2006-08-01 00:00:00

abstract：:Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two heat-sensitive and two cold-sensitive isolates were characterized. The growth of the four mutants is inhibited at their ...

journal_title：Molecular and cellular biology

authors： Moritz M,Pulaski BA,Woolford JL Jr

更新日期：1991-11-01 00:00:00

abstract：:In the current study, we were interested in exploring the molecular mechanism of circular RNA DLEU2 (circRNA-DLEU2) (hsa_circ_0000488) and microRNA 496 (miR-496), as well as PRKACB, in human acute myeloid leukemia (AML) cell activities. The RNA expression levels of circRNA-DLEU2, hsa-miR-496, and PRKACB were assessed ...

journal_title：Molecular and cellular biology

authors： Wu DM,Wen X,Han XR,Wang S,Wang YJ,Shen M,Fan SH,Zhang ZF,Shan Q,Li MQ,Hu B,Chen GQ,Lu J,Zheng YL

更新日期：2018-09-28 00:00:00

abstract：:Two-dimensional protein gels were used to systematically assess changes in gene expression in Friend erythroleukemia cells after exposure to inducers of differentiation. A rapid decrease in expression of the stress protein HSP70 was observed after exposure to inducers. The kinetics of this change suggest that it may b...

journal_title：Molecular and cellular biology

authors： Hensold JO,Housman DE

更新日期：1988-05-01 00:00:00

abstract：:The interferon (IFN)-induced double-stranded RNA-activated protein kinase PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-k...

journal_title：Molecular and cellular biology

authors： Bonnet MC,Weil R,Dam E,Hovanessian AG,Meurs EF

更新日期：2000-07-01 00:00:00

abstract：:hsa-miR-33a and hsa-miR-33b, intronic microRNAs (miRNAs) located within the sterol regulatory element-binding protein 2 and 1 genes (Srebp-2 and -1), respectively, have recently been shown to regulate lipid homeostasis in concert with their host genes. Although the functional role of miR-33a and -b has been highly inv...

journal_title：Molecular and cellular biology

authors： Goedeke L,Vales-Lara FM,Fenstermaker M,Cirera-Salinas D,Chamorro-Jorganes A,Ramírez CM,Mattison JA,de Cabo R,Suárez Y,Fernández-Hernando C

更新日期：2013-06-01 00:00:00

abstract：:Expression of the reaper gene (rpr) correlates with the initiation of apoptosis in Drosophila melanogaster. Transient expression of rpr in the lepidopteran SF-21 cell line induced apoptosis displaying nuclear condensation and fragmentation, oligonucleosomal ladder formation, cell surface blebbing, and apoptotic body f...

journal_title：Molecular and cellular biology

authors： Vucic D,Seshagiri S,Miller LK

更新日期：1997-02-01 00:00:00

abstract：:Histone acetylation plays an important role in regulating chromatin structure and thus gene expression. Here we describe the functional characterization of HDAC4, a human histone deacetylase whose C-terminal part displays significant sequence similarity to the deacetylase domain of yeast HDA1. HDAC4 is expressed in va...

journal_title：Molecular and cellular biology

authors： Wang AH,Bertos NR,Vezmar M,Pelletier N,Crosato M,Heng HH,Th'ng J,Han J,Yang XJ

更新日期：1999-11-01 00:00:00

abstract：:The tRNA methytransferase NSun2 promotes cell proliferation, but the molecular mechanism has not been elucidated. Here, we report that NSun2 regulates cyclin-dependent kinase 1 (CDK1) expression in a cell cycle-dependent manner. Knockdown of NSun2 decreased the CDK1 protein level, while overexpression of NSun2 elevate...

journal_title：Molecular and cellular biology

authors： Xing J,Yi J,Cai X,Tang H,Liu Z,Zhang X,Martindale JL,Yang X,Jiang B,Gorospe M,Wang W

更新日期：2015-12-01 00:00:00

abstract：:Normal cells do not divide indefinitely due to a process known as replicative senescence. Human cells arrest growth with a senescent phenotype when they acquire one or more critically short telomeres as a consequence of cell division. Recent evidence suggests that certain types of DNA damage, chromatin remodeling, and...

journal_title：Molecular and cellular biology

authors： Dimri GP,Itahana K,Acosta M,Campisi J

更新日期：2000-01-01 00:00:00

abstract：:Defects in mismatch repair (MMR) genes result in a mutator phenotype by inducing microsatellite instability (MI), a characteristic of hereditary nonpolyposis colorectal cancers (HNPCC) and a subset of sporadic colon tumors. Present models describing the mechanism by which germ line mutations in MMR genes predispose ki...

journal_title：Molecular and cellular biology

authors： Nicolaides NC,Littman SJ,Modrich P,Kinzler KW,Vogelstein B

更新日期：1998-03-01 00:00:00

abstract：:LAC9 is a DNA-binding protein that regulates transcription of the lactose-galactose regulon in Kluyveromyces lactis. The DNA-binding domain is composed of a zinc finger and nearby amino acids (M. M. Witte and R. C. Dickson, Mol. Cell. Biol. 8:3726-3733, 1988). The single zinc finger appears to be structurally related ...

journal_title：Molecular and cellular biology

authors： Witte MM,Dickson RC

更新日期：1990-10-01 00:00:00

abstract：:The connections between various nuclear processes and specific histone posttranslational modifications are dependent to a large extent on the acquisition of those modifications after histone synthesis. The reestablishment of histone posttranslational modifications after S phase is especially critical for H3K9 and H3K2...

journal_title：Molecular and cellular biology

authors： Zee BM,Britton LM,Wolle D,Haberman DM,Garcia BA

更新日期：2012-07-01 00:00:00

abstract：:Mammalian MEK1 and MEK2 contain a proline-rich (PR) sequence that is absent both from the yeast homologs Ste7 and Byr1 and from a recently cloned activator of the JNK/stress-activated protein kinases, SEK1/MKK4. Since this PR sequence occurs in MEKs that are regulated by Raf family enzymes but is missing from MEKs and...

journal_title：Molecular and cellular biology

authors： Catling AD,Schaeffer HJ,Reuter CW,Reddy GR,Weber MJ

更新日期：1995-10-01 00:00:00

abstract：:In vitro studies have implicated nuclear respiratory factor 1 (NRF-1) in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. We investigated here the in vivo function of NRF-1 in mammals by disrupting the gene in mice. A...

journal_title：Molecular and cellular biology

authors： Huo L,Scarpulla RC

更新日期：2001-01-01 00:00:00

abstract：:DNA elements with the CC(A/T)6GG, or CArG, motif occur in promoters that are under different regulatory controls. CArG elements from the skeletal actin, c-fos, and myogenin genes were tested for their abilities to confer tissue-specific expression on reporter genes when the individual elements were situated immediatel...

journal_title：Molecular and cellular biology

authors： Santoro IM,Walsh K

更新日期：1991-12-01 00:00:00

abstract：:The on/off state of the binary switch gene Sex-lethal (Sxl), which controls somatic sexual development in Drosophila melanogaster, is regulated at the level of alternative splicing. In males, in which the gene is off, the default splicing machinery produces nonfunctional mRNAs; in females, in which the gene is on, the...

journal_title：Molecular and cellular biology

authors： Horabin JI,Schedl P

更新日期：1993-12-01 00:00:00

abstract：:The tyrosine kinase activity of the epidermal growth factor (EGF) receptor is regulated by a truncated receptor of 100 kilodaltons (kDa) that contains the EGF-binding site but not the kinase domain. The inhibition of kinase is not due to competition for available EGF or for the kinase substrate-binding site. Chemical ...

journal_title：Molecular and cellular biology

authors： Basu A,Raghunath M,Bishayee S,Das M

更新日期：1989-02-01 00:00:00

abstract：:The Wnt signaling pathway plays a pivotal role in vertebrate early development and morphogenesis. Duplin (axis duplication inhibitor) interacts with beta-catenin and prevents its binding to Tcf, thereby inhibiting downstream Wnt signaling. Here we show that Duplin is expressed predominantly from early- to mid-stage mo...

journal_title：Molecular and cellular biology

authors： Nishiyama M,Nakayama K,Tsunematsu R,Tsukiyama T,Kikuchi A,Nakayama KI

更新日期：2004-10-01 00:00:00

abstract：:Cholera toxin (CT) activates expression of two immediate-early response genes (JE and TIS10) in quiescent BALB/c 3T3 cells. Increases in cyclic AMP (cAMP) levels in response to CT are likely responsible for the induction of TIS10 gene expression, since treatment with 8-Br-cAMP and increasing the intracellular levels o...

journal_title：Molecular and cellular biology

authors： Qureshi SA,Alexandropoulos K,Joseph CK,Spangler R,Foster DA

更新日期：1991-01-01 00:00:00

abstract：:The human genome contains a number of methyl CpG binding proteins that translate DNA methylation into a physiological response. To gain insight into the function of MBD2 and MBD3, we first applied protein tagging and mass spectrometry. We show that MBD2 and MBD3 assemble into mutually exclusive distinct Mi-2/NuRD-like...

journal_title：Molecular and cellular biology

authors： Le Guezennec X,Vermeulen M,Brinkman AB,Hoeijmakers WA,Cohen A,Lasonder E,Stunnenberg HG

更新日期：2006-02-01 00:00:00

abstract：:Nascent-polypeptide-associated complex and coactivator alpha (alpha NAC) is a protein shuttling between the nucleus and the cytoplasm. Upon phosphorylation at residue serine 43 by integrin-linked kinase, alpha NAC is translocated to the nuclei of osteoblasts, where it acts as an AP-1 coactivator to increase osteocalci...

journal_title：Molecular and cellular biology

authors： Meury T,Akhouayri O,Jafarov T,Mandic V,St-Arnaud R

更新日期：2010-01-01 00:00:00

abstract：:Tax1, a potent activator of human T-cell lymphotropic virus type 1 (HTLV-1) transcription, has been shown to modulate expression of many cellular genes. Tax1 does not bind DNA directly but regulates transcription through protein-protein interactions with sequence-specific transcription factors. Using the yeast two-hyb...

journal_title：Molecular and cellular biology

authors： Pise-Masison CA,Dittmer J,Clemens KE,Brady JN

更新日期：1997-03-01 00:00:00

abstract：:A fragment of the mouse mammary tumor virus (MMTV) promoter was reconstituted from pure histones into a dinucleosome with uniquely positioned octamer cores. Core boundaries for the in vitro-assembled dinucleosome corresponded to the observed in vivo phasing pattern for long terminal repeat nucleosomes A and B. Nuclear...

journal_title：Molecular and cellular biology

authors： Archer TK,Cordingley MG,Wolford RG,Hager GL

更新日期：1991-02-01 00:00:00

abstract：:Non-long terminal repeat (LTR) retrotransposons are major components of the higher eukaryotic genome. Most of them have two open reading frames (ORFs): ORF2 encodes mainly the endonuclease and reverse transcriptase domains, but the functional features of ORF1 remain largely unknown. We used telomere-specific non-LTR r...

journal_title：Molecular and cellular biology

authors： Matsumoto T,Hamada M,Osanai M,Fujiwara H

更新日期：2006-07-01 00:00:00

abstract：:Bach2 is a B-cell- and neuron-specific transcription repressor that forms heterodimers with the Maf-related oncoproteins. We show here that Bach2 activates transcription by interacting with its novel partner MAZR. MAZR was isolated by the yeast two-hybrid screen using the BTB/POZ domain of Bach2 as bait. Besides the B...

journal_title：Molecular and cellular biology

authors： Kobayashi A,Yamagiwa H,Hoshino H,Muto A,Sato K,Morita M,Hayashi N,Yamamoto M,Igarashi K

更新日期：2000-03-01 00:00:00

abstract：:The minichromosome maintenance protein 10 (Mcm10) is an evolutionarily conserved factor that is essential for replication initiation and elongation. Mcm10 is part of the eukaryotic replication fork and interacts with a variety of proteins, including the Mcm2-7 helicase and DNA polymerase alpha/primase complexes. A mot...

journal_title：Molecular and cellular biology

authors： Das-Bradoo S,Ricke RM,Bielinsky AK

更新日期：2006-07-01 00:00:00

abstract：:The Mauriceville and Varkud mitochondrial plasmids of Neurospora spp. are closely related, small circular DNAs that propagate via an RNA intermediate and reverse transcription. Although the plasmids ordinarily replicate autonomously, they can also integrate into mitochondrial DNA (mtDNA), yielding defective mtDNAs tha...

journal_title：Molecular and cellular biology

authors： Chiang CC,Kennell JC,Wanner LA,Lambowitz AM

更新日期：1994-10-01 00:00:00

abstract：:The rad18 gene of Schizosaccharomyces pombe is an essential gene that is involved in several different DNA repair processes. Rad18 (Smc6) is a member of the structural maintenance of chromosomes (SMC) family and, together with its SMC partner Spr18 (Smc5), forms the core of a high-molecular-weight complex. We show her...

journal_title：Molecular and cellular biology

authors： Sergeant J,Taylor E,Palecek J,Fousteri M,Andrews EA,Sweeney S,Shinagawa H,Watts FZ,Lehmann AR

更新日期：2005-01-01 00:00:00

abstract：:Bona fide apoptosis in rat and human leukemia cells, rat thymocytes, and bovine endothelial cells was accompanied by limited and specific cleavage of polysome-associated and monosome-associated 28S rRNA, with 18S rRNA being spared. Specific 28S rRNA cleavage was observed in all instances of apoptotic death accompanied...

journal_title：Molecular and cellular biology

authors： Houge G,Robaye B,Eikhom TS,Golstein J,Mellgren G,Gjertsen BT,Lanotte M,Døskeland SO

更新日期：1995-04-01 00:00:00