Regulation of a senescence checkpoint response by the E2F1 transcription factor and p14(ARF) tumor suppressor.

abstract：:The 2 micron plasmid of Saccharomyces cerevisiae codes for a site-specific recombinase, the FLP protein, that catalyzes efficient recombination across two 599-base-pair (bp) inverted repeats of the plasmid DNA both in vivo and in vitro. We analyzed the interaction of the purified FLP protein with the target sequences ...

journal_title：Molecular and cellular biology

authors： Andrews BJ,McLeod M,Broach J,Sadowski PD

更新日期：1986-07-01 00:00:00

abstract：:macroH2A histone variants have been implicated to function in gene silencing by several studies, including ones showing a preferential association of macroH2A on the inactive X chromosome. To examine macroH2A function in vivo, we knocked out macroH2A1. macroH2A1 knockout mice are viable and fertile. A broad screen of ...

journal_title：Molecular and cellular biology

authors： Changolkar LN,Costanzi C,Leu NA,Chen D,McLaughlin KJ,Pehrson JR

更新日期：2007-04-01 00:00:00

abstract：:The mouse myosin light-chain 1A (MLC1A) gene, expressed in the atria of the adult heart, is one of the first muscle genes to be activated when skeletal as well as cardiac muscles form in the embryo. It is also transcribed in skeletal muscle cell lines at the onset of differentiation. Transient transfection assays of m...

journal_title：Molecular and cellular biology

authors： Catala F,Wanner R,Barton P,Cohen A,Wright W,Buckingham M

更新日期：1995-08-01 00:00:00

abstract：:Nuclear expression and consequent biological action of the eukaryotic NF-kappa B transcription factor complex are tightly regulated through its cytoplasmic retention by an ankyrin-rich inhibitory protein termed I kappa B alpha. I kappa B alpha specifically binds to and masks the nuclear localization signal of the RelA...

journal_title：Molecular and cellular biology

authors： Sun S,Elwood J,Greene WC

更新日期：1996-03-01 00:00:00

abstract：:The nuclear receptor peroxisome proliferator-activated receptor gamma regulates adipose differentiation and systemic insulin signaling via ligand-dependent transcriptional activation of target genes. However, the identities of the biologically relevant target genes are largely unknown. Here we describe the isolation a...

journal_title：Molecular and cellular biology

authors： Yoon JC,Chickering TW,Rosen ED,Dussault B,Qin Y,Soukas A,Friedman JM,Holmes WE,Spiegelman BM

更新日期：2000-07-01 00:00:00

abstract：:We previously purified and characterized functionally the Escherichia coli-expressed product of the human subgroup C adenovirus E1A 13S mRNA (B. Ferguson, N. Jones, J. Richter, and M. Rosenberg, Science 224:1343-1346, 1984; B. Krippl, B. Ferguson, M. Rosenberg, and H. Westphal, Proc. Natl. Acad. Sci. USA 81:6988-6992,...

journal_title：Molecular and cellular biology

authors： Ferguson B,Krippl B,Andrisani O,Jones N,Westphal H,Rosenberg M

更新日期：1985-10-01 00:00:00

abstract：:Drosophila sine oculis and eyes absent genes synergize in compound-eye formation. The murine homologues of these genes, Six and Eya, respectively, show overlapping expression patterns during development. We hypothesized that Six and Eya proteins cooperate to regulate their target genes. Cotransfection assays were perf...

journal_title：Molecular and cellular biology

authors： Ohto H,Kamada S,Tago K,Tominaga SI,Ozaki H,Sato S,Kawakami K

更新日期：1999-10-01 00:00:00

abstract：:R2 non-long terminal repeat retrotransposable elements insert at a unique site in the 28S rRNA genes of insects. The protein encoded by the single open reading frame of R2 is capable of conducting the initial steps of its integration in vitro. The protein nicks the noncoding strand of the 28S target DNA (the strand wh...

journal_title：Molecular and cellular biology

authors： Luan DD,Eickbush TH

更新日期：1996-09-01 00:00:00

abstract：:The death-associated protein (DAP) kinase family includes three protein kinases, DAP kinase, DAP kinase-related protein 1, and ZIP kinase, which display 80% amino acid identity within their catalytic domains and are functionally linked to common subcellular changes occurring during cell death, such as the process of m...

journal_title：Molecular and cellular biology

authors： Shani G,Marash L,Gozuacik D,Bialik S,Teitelbaum L,Shohat G,Kimchi A

更新日期：2004-10-01 00:00:00

abstract：:The expression of chromosomally integrated transgenes usually varies greatly among independent transfectants. This variability in transgene expression has led to the definition of locus control regions (LCRs) as elements which render expression consistent. Analyses of expression in single cells revealed that the expre...

journal_title：Molecular and cellular biology

authors： Ronai D,Berru M,Shulman MJ

更新日期：1999-10-01 00:00:00

abstract：:Wnt/β-catenin signaling is a critical regulator of skeletal physiology. However, previous studies have mainly focused on its roles in osteoblasts, while its specific function in osteoclasts is unknown. This is a clinically important question because neutralizing antibodies against Wnt antagonists are promising new dru...

journal_title：Molecular and cellular biology

authors： Wei W,Zeve D,Suh JM,Wang X,Du Y,Zerwekh JE,Dechow PC,Graff JM,Wan Y

更新日期：2011-12-01 00:00:00

abstract：:To interrogate endogenous p21(WAF1/CIP1) (p21) promoter activity under basal conditions and in response to various forms of stress, knock-in imaging reporter mice in which expression of firefly luciferase (FLuc) was placed under the control of the endogenous p21 promoter within the Cdkn1a gene locus were generated. Bi...

journal_title：Molecular and cellular biology

authors： Tinkum KL,Marpegan L,White LS,Sun J,Herzog ED,Piwnica-Worms D,Piwnica-Worms H

更新日期：2011-09-01 00:00:00

abstract：:Sp1-like proteins are defined by three highly homologous C(2)H(2) zinc finger motifs that bind GC-rich sequences found in the promoters of a large number of genes essential for mammalian cell homeostasis. Here we report that TIEG2, a transforming growth factor beta-inducible Sp1-like protein with antiproliferative fun...

journal_title：Molecular and cellular biology

authors： Zhang JS,Moncrieffe MC,Kaczynski J,Ellenrieder V,Prendergast FG,Urrutia R

更新日期：2001-08-01 00:00:00

abstract：:Despite detailed knowledge of the components of the spindle assembly checkpoint, a molecular explanation of how cells die after prolonged spindle checkpoint activation, and thus how microtubule inhibitors and other antimitotic drugs ultimately elicit their lethal effects, has yet to emerge. Mitotically arrested cells ...

journal_title：Molecular and cellular biology

authors： Terrano DT,Upreti M,Chambers TC

更新日期：2010-02-01 00:00:00

abstract：:Cellular differentiation is governed by changes in gene expression, but at the same time, a cell's identity needs to be maintained through multiple cell divisions during maturation. In myeloid cell lines, retinoids induce gene expression and a well-characterized two-step lineage-specific differentiation. To identify m...

journal_title：Molecular and cellular biology

authors： Balint BL,Szanto A,Madi A,Bauer UM,Gabor P,Benko S,Puskás LG,Davies PJ,Nagy L

更新日期：2005-07-01 00:00:00

abstract：:Leukemia results from the expansion of self-renewing hematopoietic cells that are thought to contain mutations that contribute to disease initiation and progression. Studies of the gene expression profiles of human acute myeloid leukemia samples has allowed their classification based on the presence of translocations ...

journal_title：Molecular and cellular biology

authors： Walter MJ,Park JS,Lau SK,Li X,Lane AA,Nagarajan R,Shannon WD,Ley TJ

更新日期：2004-12-01 00:00:00

abstract：:The interleukin enhancer binding factors ILF2 (NF45) and ILF3 (NF90/NF110) have been implicated in various cellular pathways, such as transcription, microRNA (miRNA) processing, DNA repair, and translation, in mammalian cells. Using tandem affinity purification, we identified human NF45 and NF90 as components of precu...

journal_title：Molecular and cellular biology

authors： Wandrey F,Montellese C,Koos K,Badertscher L,Bammert L,Cook AG,Zemp I,Horvath P,Kutay U

更新日期：2015-10-01 00:00:00

abstract：:Placental development initially occurs in a low-oxygen (O2) or hypoxic environment. In this report we show that two hypoxia-inducible factors (HIFs), HIF1alpha and HIF2alpha, are essential for determining murine placental cell fates. HIF is a heterodimer composed of HIFalpha and HIFbeta (ARNT) subunits. Placentas from...

journal_title：Molecular and cellular biology

authors： Cowden Dahl KD,Fryer BH,Mack FA,Compernolle V,Maltepe E,Adelman DM,Carmeliet P,Simon MC

更新日期：2005-12-01 00:00:00

abstract：:We have analyzed the structures and locations of small nuclear RNA (snRNA) precursors at various stages in their synthesis and maturation. In the nuclei of pulse-labeled Xenopus laevis oocytes, we detected snRNAs that were longer than their mature forms at their 3' ends by up to 10 nucleotides. Analysis of the 5' caps...

journal_title：Molecular and cellular biology

authors： Neuman de Vegvar HE,Dahlberg JE

更新日期：1990-07-01 00:00:00

abstract：:Essential for embryonic development, the polycomb group protein enhancer of zeste homolog 2 (EZH2) is overexpressed in breast and prostate cancers and is implicated in the growth and aggression of the tumors. The tumorigenic mechanism underlying EZH2 overexpression is largely unknown. It is believed that EZH2 exerts i...

journal_title：Molecular and cellular biology

authors： Shi B,Liang J,Yang X,Wang Y,Zhao Y,Wu H,Sun L,Zhang Y,Chen Y,Li R,Zhang Y,Hong M,Shang Y

更新日期：2007-07-01 00:00:00

abstract：:Oxygen-dependent proteolysis is the primary means of regulating the hypoxia-inducible factor (HIF) family of transcription factors. The alpha-subunit of HIF factor 1 (HIF-1) contains two highly conserved oxygen-dependent degradation domains (402 ODD and 564 ODD), each of which includes a proline that is hydroxylated i...

journal_title：Molecular and cellular biology

authors： Chan DA,Sutphin PD,Yen SE,Giaccia AJ

更新日期：2005-08-01 00:00:00

abstract：:The synthesis of 60S ribosomal subunits in Saccharomyces cerevisiae requires Tif6p, the yeast homologue of mammalian eukaryotic translation initiation factor 6 (eIF6). In the present work, we have isolated a protein kinase from rabbit reticulocyte lysates on the basis of its ability to phosphorylate recombinant human ...

journal_title：Molecular and cellular biology

authors： Basu U,Si K,Deng H,Maitra U

更新日期：2003-09-01 00:00:00

abstract：:Although it is generally believed that the 60- and 81-base-pair (60/81-bp) repeats of the Xenopus laevis ribosomal DNA (rDNA) spacer are position-independent transcriptional enhancers, this has not been shown directly. We have now developed a critical assay which proves that the 60/81-bp repeats do, in fact, stimulate...

journal_title：Molecular and cellular biology

authors： Pape LK,Windle JJ,Mougey EB,Sollner-Webb B

更新日期：1989-11-01 00:00:00

abstract：:Our previous studies of Saccharomyces cerevisiae described a gene repression mechanism where the transcription of intergenic noncoding DNA (ncDNA) (SRG1) assembles nucleosomes across the promoter of the adjacent SER3 gene that interfere with the binding of transcription factors. To investigate the role of histones in ...

journal_title：Molecular and cellular biology

authors： Hainer SJ,Martens JA

更新日期：2011-09-01 00:00:00

abstract：:Tyrosine phosphorylation-dependent signaling, as mediated by members of the epidermal growth factor receptor (EGFR) family (ErbB1 to -4) of protein tyrosine kinases (PTKs), Src family PTKs (SFKs), and cytokines such as interleukin-6 (IL-6) that signal via signal transducer and activator of transcription 3 (STAT3), is ...

journal_title：Molecular and cellular biology

authors： Shields BJ,Wiede F,Gurzov EN,Wee K,Hauser C,Zhu HJ,Molloy TJ,O'Toole SA,Daly RJ,Sutherland RL,Mitchell CA,McLean CA,Tiganis T

更新日期：2013-02-01 00:00:00

abstract：:Biochemical experiments indicate that the general transcription factor IIB (TFIIB) can interact directly with acidic activation domains and that activators can stimulate transcription by increasing recruitment of TFIIB to promoters. For promoters at which recruitment of TFIIB to promoters is limiting in vivo, one woul...

journal_title：Molecular and cellular biology

authors： Chou S,Struhl K

更新日期：1997-12-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is an inherited motor neuron disease caused by homozygous loss of the Survival Motor Neuron 1 (SMN1) gene. In the absence of SMN1, inefficient inclusion of exon 7 in transcripts from the nearly identical SMN2 gene results in ubiquitous SMN decrease but selective motor neuron degeneration....

journal_title：Molecular and cellular biology

authors： Ruggiu M,McGovern VL,Lotti F,Saieva L,Li DK,Kariya S,Monani UR,Burghes AH,Pellizzoni L

更新日期：2012-01-01 00:00:00

abstract：:We have constructed a size variant of the Drosophila hsp28 gene by deleting 207 base pairs of the protein coding region, beginning 33 base pairs downstream of the ATG protein initiation codon. After transformation of Drosophila melanogaster rosy (ry506) flies with this altered gene, using the P transposable element sy...

journal_title：Molecular and cellular biology

authors： Hoffman EP,Corces VG

更新日期：1984-12-01 00:00:00

abstract：:DNA mismatch repair maintains genomic stability by detecting and correcting mispaired DNA sequences and by signaling cell death when DNA repair fails. The mechanism by which mismatch repair coordinates DNA damage and repair with cell survival or death is not understood, but it suggests the need for regulation. Since t...

journal_title：Molecular and cellular biology

authors： Wu X,Platt JL,Cascalho M

更新日期：2003-05-01 00:00:00

abstract：:The Stunted protein (StuAp) is a member of a family of transcription factors that regulate fungal development and cell cycle progression. Regulated stuA gene expression is required for correct cell pattern formation during asexual reproduction (conidiation) and for initiation of the sexual reproductive cycle in Asperg...

journal_title：Molecular and cellular biology

authors： Wu J,Miller BL

更新日期：1997-10-01 00:00:00