Abstract:
:The tRNA methytransferase NSun2 promotes cell proliferation, but the molecular mechanism has not been elucidated. Here, we report that NSun2 regulates cyclin-dependent kinase 1 (CDK1) expression in a cell cycle-dependent manner. Knockdown of NSun2 decreased the CDK1 protein level, while overexpression of NSun2 elevated it without altering CDK1 mRNA levels. Further studies revealed that NSun2 methylated CDK1 mRNA in vitro and in cells and that methylation by NSun2 enhanced CDK1 translation. Importantly, NSun2-mediated regulation of CDK1 expression had an impact on the cell division cycle. These results provide new insight into the regulation of CDK1 during the cell division cycle.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Xing J,Yi J,Cai X,Tang H,Liu Z,Zhang X,Martindale JL,Yang X,Jiang B,Gorospe M,Wang Wdoi
10.1128/MCB.00742-15subject
Has Abstractpub_date
2015-12-01 00:00:00pages
4043-52issue
23eissn
0270-7306issn
1098-5549pii
MCB.00742-15journal_volume
35pub_type
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