Abstract:
:The human and murine MOK2 ortholog genes that are preferentially expressed in brain and testis tissues encode two different Krüppel-like zinc finger proteins. In this paper, we show that the MOK2 proteins are mainly associated with nuclear ribonucleoprotein components, including the nucleoli and extranucleolar structures, and exhibit specific RNA homopolymer binding activities. Moreover, we have identified an identical 18-bp specific DNA binding sequence for both MOK2 proteins using a pool of random sequence oligonucleotides. The DNA binding domain is localized in the seven adjacent zinc finger motifs, which show 94% identity between human and murine proteins. Taken together, these results establish that the MOK2 proteins are able to recognize both DNA and RNA through their zinc fingers. This dual affinity and the subnuclear localization suggest that MOK2 may play roles in transcription, as well as in the posttranscriptional regulation processes of specific genes.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Arranz V,Harper F,Florentin Y,Puvion E,Kress M,Ernoult-Lange Mdoi
10.1128/mcb.17.4.2116subject
Has Abstractpub_date
1997-04-01 00:00:00pages
2116-26issue
4eissn
0270-7306issn
1098-5549journal_volume
17pub_type
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