The survival of motor neurons protein determines the capacity for snRNP assembly: biochemical deficiency in spinal muscular atrophy.

abstract：:Drugs that produce covalent interstrand cross-links (ICLs) in DNA remain central to the treatment of cancer, but the cell cycle checkpoints activated by ICLs have received little attention. We have used the fission yeast, Schizosaccharomyces pombe, to elucidate the checkpoint responses to the ICL-inducing anticancer d...

journal_title：Molecular and cellular biology

authors： Lambert S,Mason SJ,Barber LJ,Hartley JA,Pearce JA,Carr AM,McHugh PJ

更新日期：2003-07-01 00:00:00

abstract：:The xCR1 protein is a maternal determinant and cofactor for nodal signaling in vertebrate embryos. The xCR1 protein accumulates specifically in the animal cells of Xenopus embryos, but maternal xCR1 mRNA is distributed equally throughout all embryonic cells. Here, we show that vegetal cell-specific translational repre...

journal_title：Molecular and cellular biology

authors： Zhang Y,Forinash KD,McGivern J,Fritz B,Dorey K,Sheets MD

更新日期：2009-07-01 00:00:00

abstract：:The mechanism(s) and site(s) of action of volatile inhaled anesthetics are unknown in spite of the clinical use of these agents for more than 150 years. In the present study, the model eukaryote Saccharomyces cerevisiae was used to investigate the action of anesthetic agents because of its powerful molecular genetics....

journal_title：Molecular and cellular biology

authors： Keil RL,Wolfe D,Reiner T,Peterson CJ,Riley JL

更新日期：1996-07-01 00:00:00

abstract：:A pool of Candida albicans RsaI fragments cloned onto a vector containing pBR322 sequences and the Candida ADE2 gene was used to transform a Candida ade2 mutant to adenine protrophy. A potential autonomously replicating sequence (ARS) in Candida DNA was identified by two criteria: instability of the selectable marker ...

journal_title：Molecular and cellular biology

authors： Kurtz MB,Cortelyou MW,Miller SM,Lai M,Kirsch DR

更新日期：1987-01-01 00:00:00

abstract：:Transfection experiments with constructs containing various 5'-deleted fragments of the human lipoprotein lipase (LPL) promoter and the chloramphenicol acetyltransferase reporter gene revealed an LPL silencer element (LSE) in the region of nucleotides -225 to -81 of the LPL gene that functioned in Chinese hamster ovar...

journal_title：Molecular and cellular biology

authors： Tanuma Y,Nakabayashi H,Esumi M,Endo H

更新日期：1995-01-01 00:00:00

abstract：:The inducer of short transcripts, or IST, is an unusual transcriptional element located downstream of the human immunodeficiency virus type 1 (HIV-1) promoter. IST activates HIV-1 transcription, but the resulting RNAs are short and end at approximately position +59. IST, therefore, appears to promote the formation of ...

journal_title：Molecular and cellular biology

authors： Sheldon M,Ratnasabapathy R,Hernandez N

更新日期：1993-02-01 00:00:00

abstract：:We demonstrate that the immunoglobulin heavy-chain enhancer functions as the promoter for I mu sterile transcription. The enhancer itself, when placed 5' to the bacterial cat or neo genes, is able to direct transcription by using heterogeneous start sites that are generally the same as those found with bona fide I mu ...

journal_title：Molecular and cellular biology

authors： Su LK,Kadesch T

更新日期：1990-06-01 00:00:00

abstract：:Several Epstein-Barr virus (EBV)-negative Burkitt lymphoma-derived cell lines (for example, BL41 and Ramos) are extremely sensitive to genotoxic drugs despite being functionally null for the tumor suppressor p53. They rapidly undergo apoptosis, largely from G(2)/M of the cell cycle. 5-bromo-2'-deoxyuridine labeling ex...

journal_title：Molecular and cellular biology

authors： Wade M,Allday MJ

更新日期：2000-02-01 00:00:00

abstract：:Although globin mRNAs are considered prototypes of highly stable messages, the mechanisms responsible for their longevity remain largely undefined. As an initial step in identifying potential cis-acting elements or structures which contribute to their stability, we analyzed the defect in expression of a naturally occu...

journal_title：Molecular and cellular biology

authors： Weiss IM,Liebhaber SA

更新日期：1994-12-01 00:00:00

abstract：:The role of chromatin-remodeling factors in transcription is well established, but the link between chromatin-remodeling complexes and DNA repair remains unexplored. Human Rvb1 and Rvb2 are highly conserved AAA(+) ATP binding proteins that are part of various chromatin-remodeling complexes, such as Ino80, SNF2-related...

journal_title：Molecular and cellular biology

authors： Jha S,Shibata E,Dutta A

更新日期：2008-04-01 00:00:00

abstract：:The cellular mechanisms involved in chronic inflammatory processes are poorly understood. This is especially true for the role of macrophages, which figure prominently in the inflammatory response. Two proteins, MRP8 and MRP14, which are expressed in infiltrate macrophages during inflammatory reactions but not in norm...

journal_title：Molecular and cellular biology

authors： Lagasse E,Clerc RG

更新日期：1988-06-01 00:00:00

abstract：:Calcineurin, or PP2B, plays a critical role in mediating Ca2+-dependent signaling in many cell types. In yeast cells, this highly conserved protein phosphatase regulates aspects of ion homeostasis and cell wall synthesis. We show that calcineurin mutants are sensitive to high concentrations of Mn2+ and identify two ge...

journal_title：Molecular and cellular biology

authors： Pozos TC,Sekler I,Cyert MS

更新日期：1996-07-01 00:00:00

abstract：:IkappaB kinases (IKKalpha and IKKbeta) are key components of the IKK complex that mediates activation of the transcription factor NF-kappaB in response to extracellular stimuli such as inflammatory cytokines, viral and bacterial infection, and UV irradiation. Although NF-kappaB-inducing kinase (NIK) interacts with and...

journal_title：Molecular and cellular biology

authors： Nemoto S,DiDonato JA,Lin A

更新日期：1998-12-01 00:00:00

abstract：:We have characterized the role of YPR128cp, the orthologue of human PMP34, in fatty acid metabolism and peroxisomal proliferation in Saccharomyces cerevisiae. YPR128cp belongs to the mitochondrial carrier family (MCF) of solute transporters and is localized in the peroxisomal membrane. Disruption of the YPR128c gene r...

journal_title：Molecular and cellular biology

authors： van Roermund CW,Drissen R,van Den Berg M,Ijlst L,Hettema EH,Tabak HF,Waterham HR,Wanders RJ

更新日期：2001-07-01 00:00:00

abstract：:The small subunit of ribonucleotide reductase in Saccharomyces cerevisiae (RNR2) was induced 3- to 20-fold by a variety of DNA-damaging agents. Induction of the RNR2 transcript by at least one of these agents, methyl methanesulfonate, did not require protein synthesis. To identify sequences involved in the regulation ...

journal_title：Molecular and cellular biology

authors： Hurd HK,Roberts JW

更新日期：1989-12-01 00:00:00

abstract：:The first processing event that mouse pre-rRNA undergoes occurs within the external transcribed spacer and is efficiently reproduced in vitro. Analysis with nondenaturing polyacrylamide gels revealed the formation of heparin-resistant complexes of retarded electrophoretic mobility on the substrate rRNA. The specificit...

journal_title：Molecular and cellular biology

authors： Kass S,Sollner-Webb B

更新日期：1990-09-01 00:00:00

abstract：:Nucleolin is a ubiquitous multifunctional protein involved in preribosome assembly and associated with both nucleolar chromatin in interphase and nucleolar organizer regions on metaphasic chromosomes in mitosis. Extensive nucleolin phosphorylation by a casein kinase (CKII) occurs on serine in growing cells. Here we re...

journal_title：Molecular and cellular biology

authors： Belenguer P,Caizergues-Ferrer M,Labbé JC,Dorée M,Amalric F

更新日期：1990-07-01 00:00:00

abstract：:Complete replication of the genome is an essential prerequisite for normal cell division, but a variety of factors can block the replisome, triggering replication stress and potentially causing mutation or cell death. The cellular response to replication stress involves recruitment of proteins to stabilize the replica...

journal_title：Molecular and cellular biology

authors： Her J,Ray C,Altshuler J,Zheng H,Bunting SF

更新日期：2018-03-29 00:00:00

abstract：:Y-27632, an inhibitor of the Rho-associated kinase ROCK, is a therapeutic lead for Huntington disease (HD). The downstream targets that mediate its inhibitory effects on huntingtin (Htt) aggregation and toxicity are unknown. We have identified profilin, a small actin-binding factor that also interacts with Htt, as bei...

journal_title：Molecular and cellular biology

authors： Shao J,Welch WJ,Diprospero NA,Diamond MI

更新日期：2008-09-01 00:00:00

abstract：:Transfection of a functional cloned p53 gene into an L12 p53 nonproducer cell line efficiently reconstituted p53 expression. The p53 protein synthesized in these clones was indistinguishable from that occurring naturally in tumor cells. When a p53 cDNA clone was used instead, we observed that the L12-derived clones ex...

journal_title：Molecular and cellular biology

authors： Arai N,Nomura D,Yokota K,Wolf D,Brill E,Shohat O,Rotter V

更新日期：1986-09-01 00:00:00

abstract：:It is well accepted that for transcriptional silencing in budding yeast, the evolutionarily conserved lysine deacetylase Sir2, in concert with its partner proteins Sir3 and Sir4, establishes a chromatin structure that prevents RNA polymerase II (Pol II) transcription. However, the mechanism of repression remains contr...

journal_title：Molecular and cellular biology

authors： Gao L,Gross DS

更新日期：2008-06-01 00:00:00

abstract：:The Ace2p and Swi5p zinc finger proteins have nearly identical DNA-binding domains, yet in vivo they activate transcription of different genes, CTS1 and HO. We now demonstrate that Ace2p and Swi5p recognize sites in the CTS1 and HO promoters with the same affinities, raising the question of how promoter specificity is...

journal_title：Molecular and cellular biology

authors： Dohrmann PR,Voth WP,Stillman DJ

更新日期：1996-04-01 00:00:00

abstract：:The punc gene, encoding a member of the neural cell adhesion molecule family expressed in the developing central nervous system, limbs, and inner ear, was identified. To extend studies of the normal expression pattern of punc and to determine its function, a mouse strain bearing a lacZ/neo insertion in a 5' coding exo...

journal_title：Molecular and cellular biology

authors： Yang W,Li C,Mansour SL

更新日期：2001-09-01 00:00:00

abstract：:We find that during embryogenesis the expression of HMGN1, a nuclear protein that binds to nucleosomes and reduces the compaction of the chromatin fiber, is progressively down-regulated throughout the entire embryo, except in committed but continuously renewing cell types, such as the basal layer of the epithelium. In...

journal_title：Molecular and cellular biology

authors： Furusawa T,Lim JH,Catez F,Birger Y,Mackem S,Bustin M

更新日期：2006-01-01 00:00:00

abstract：:mRNA deadenylation is a key process in the regulation of translation and mRNA turnover. In Saccharomyces cerevisiae, deadenylation is primarily carried out by the Ccr4p and Caf1p/Pop2p subunits of the Ccr4-Not complex, which is conserved in eukaryotes including humans. Here we have identified an unconventional human C...

journal_title：Molecular and cellular biology

authors： Wagner E,Clement SL,Lykke-Andersen J

更新日期：2007-03-01 00:00:00

abstract：:The contribution of erythropoietin to the differentiation of the red blood cell lineage remains elusive, and the demonstration of a molecular link between erythropoietin and the transcription of genes associated with erythroid differentiation is lacking. In erythroid cells, expression of the tissue inhibitor of matrix...

journal_title：Molecular and cellular biology

authors： Kadri Z,Maouche-Chretien L,Rooke HM,Orkin SH,Romeo PH,Mayeux P,Leboulch P,Chretien S

更新日期：2005-09-01 00:00:00

abstract：:Histone H3 lysine 9 (H3K9) methylation has broad roles in transcriptional repression, gene silencing, maintenance of heterochromatin, and epigenetic inheritance of heterochromatin. Using Xenopus laevis oocytes, we have previously shown that targeting G9a, an H3K9 histone methyltransferase, to chromatin increases H3K9 ...

journal_title：Molecular and cellular biology

authors： Stewart MD,Sommerville J,Wong J

更新日期：2006-09-01 00:00:00

abstract：:Activation of transcription can occur by the facilitated recruitment of TFIID to promoters by gene-specific activators. To investigate the role of TFIIA in TFIID recruitment in vivo, we exploited a class of yeast TATA-binding protein (TBP) mutants that is activation and DNA binding defective. We found that co-overexpr...

journal_title：Molecular and cellular biology

authors： Liu Q,Gabriel SE,Roinick KL,Ward RD,Arndt KM

更新日期：1999-12-01 00:00:00

abstract：:Mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) has been shown to play a critical role in mediating the feedback control of MAP kinase cascades in a variety of cellular processes, including proliferation and stress responsiveness. Although MKP-1 expression is induced by a broad array of extracellular stim...

journal_title：Molecular and cellular biology

authors： Li J,Gorospe M,Hutter D,Barnes J,Keyse SM,Liu Y

更新日期：2001-12-01 00:00:00

abstract：:Unlike other eukaryotes, the protein-coding genes of Trypanosoma cruzi are arranged in large polycistronic gene clusters transcribed by polymerase II (Pol II). Thus, it is thought that trypanosomes rely solely on posttranscriptional processes to regulate gene expression. Here, we show that the glucosylated thymine DNA...

journal_title：Molecular and cellular biology

authors： Ekanayake DK,Minning T,Weatherly B,Gunasekera K,Nilsson D,Tarleton R,Ochsenreiter T,Sabatini R

更新日期：2011-04-01 00:00:00