Cdc37 promotes the stability of protein kinases Cdc28 and Cak1.

abstract：:The Cyp cell line consists of mouse cells transformed by a thermosensitive polyomavirus (Py) genome and routinely propagated at 39 degrees C. Cyp cells are readily induced to synthesize free Py DNA by being transferred to 33 degrees C. In one subclone (C12/a1/S48, or S48) of this line, such induction resulted in the i...

journal_title：Molecular and cellular biology

authors： Huberdeau D,Sylla BS,Herring-Gillam E,Bourgaux-Ramoisy D,Bourgaux P

更新日期：1985-10-01 00:00:00

abstract：:The regulation of telomerase reverse transcriptase (TERT) plays an important role in the proliferative capacity and survival of cells. Here, we report that exogenously as well as endogenously induced oxidative stress leads to translocation of endogenous as well as overexpressed human TERT from the nucleus into the cyt...

journal_title：Molecular and cellular biology

authors： Haendeler J,Hoffmann J,Brandes RP,Zeiher AM,Dimmeler S

更新日期：2003-07-01 00:00:00

abstract：:To examine a role for focal adhesion kinase (FAK) in cardiac morphogenesis, we generated a line of mice with a conditional deletion of FAK in nkx2-5-expressing cells (herein termed FAKnk mice). FAKnk mice died shortly after birth, likely resulting from a profound subaortic ventricular septal defect and associated mala...

journal_title：Molecular and cellular biology

authors： Hakim ZS,DiMichele LA,Doherty JT,Homeister JW,Beggs HE,Reichardt LF,Schwartz RJ,Brackhan J,Smithies O,Mack CP,Taylor JM

更新日期：2007-08-01 00:00:00

abstract：:Histone N-terminal tails are central to the processes that modulate nucleosome structure and function. We have studied the contribution of core histone tails to the structure of a single nucleosome and to a histone (H3-H4)(2) tetrameric particle assembled on a topologically constrained DNA minicircle. The effect of hi...

journal_title：Molecular and cellular biology

authors： Morales V,Richard-Foy H

更新日期：2000-10-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) are unipotent germ cells that are at the foundation of spermatogenesis and male fertility. However, the underlying molecular mechanisms governing SSC stemness and growth properties remain elusive. We have recently identified chromodomain helicase/ATPase DNA binding protein 1-like (Chd1...

journal_title：Molecular and cellular biology

authors： Liu SS,Maguire EM,Bai YS,Huang L,Liu Y,Xu L,Fauzi I,Zhang SQ,Xiao Q,Ma NF

更新日期：2019-02-04 00:00:00

abstract：:The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for t...

journal_title：Molecular and cellular biology

authors： Hosler BA,Rogers MB,Kozak CA,Gudas LJ

更新日期：1993-05-01 00:00:00

abstract：:We have studied the abilities of different transactivation domains to stimulate the initiation and elongation (postinitiation) steps of RNA polymerase II transcription in vivo. Nuclear run-on and RNase protection analyses revealed three classes of activation domains: Sp1 and CTF stimulated initiation (type I); human i...

journal_title：Molecular and cellular biology

authors： Blau J,Xiao H,McCracken S,O'Hare P,Greenblatt J,Bentley D

更新日期：1996-05-01 00:00:00

abstract：:When exposed to 5-azacytidine, marrow stromal cells from active long-term marrow cultures and cell lines derived from simian virus 40-transformed stromal cells rapidly upregulated c-abl and interleukin-6 transcripts while downregulating the expression of collagen I, a major matrix protein. Similar effects occurred wit...

journal_title：Molecular and cellular biology

authors： Andrews DF 3rd,Nemunaitis J,Tompkins C,Singer JW

更新日期：1989-06-01 00:00:00

abstract：:The HOX11 homeobox gene was first identified through studies of the t(7;10) and t(10;14) chromosomal translocations of acute T-cell leukemia. In addition, analysis of Hox11-/- mice has demonstrated a critical role for this gene in murine spleen development. A possible mode of in vivo function for the HOX11 protein in ...

journal_title：Molecular and cellular biology

authors： Masson N,Greene WK,Rabbitts TH

更新日期：1998-06-01 00:00:00

abstract：:Chromosomal rearrangements associated with one Ty1 element in the iso-1-cytochrome c (CYC1) region of Saccharomyces cerevisiae yeast cells were examined. Most of the rearrangements were deletions of the three linked genes, CYC1, OSM1, and RAD7, and resulted from recombination involving the single Ty1 element and a sol...

journal_title：Molecular and cellular biology

authors： Downs KM,Brennan G,Liebman SW

更新日期：1985-12-01 00:00:00

abstract：:Eukaryotic flagella are complex organelles composed of more than 200 polypeptides. Little is known about the regulatory mechanisms governing synthesis of the flagellar protein subunits and their assembly into this complex organelle. The unicellular green alga Chlamydomonas reinhardtii is the premier experimental model...

journal_title：Molecular and cellular biology

authors： Periz G,Keller LR

更新日期：1997-07-01 00:00:00

abstract：:V(D)J recombination requires binding and synapsis of a complementary (12/23) pair of recombination signal sequences (RSSs) by the RAG1 and RAG2 proteins, aided by a high-mobility group protein, HMG1 or HMG2. Double-strand DNA cleavage within this synaptic, or paired, complex is thought to involve DNA distortion or mel...

journal_title：Molecular and cellular biology

authors： Ciubotaru M,Schatz DG

更新日期：2004-10-01 00:00:00

abstract：:The product of the c-myc proto-oncogene is an important positive regulator of cell growth and proliferation. Recently, c-Myc has also been demonstrated to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. To further examine Myc-induced apoptosis, we coexpressed the proto-oncoge...

journal_title：Molecular and cellular biology

authors： Wagner AJ,Small MB,Hay N

更新日期：1993-04-01 00:00:00

abstract：:Tie1 is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. Genetic studies suggest that Tie1 promotes endothelial cell survival, but other studies have suggested that the Tie1 kinase has little to no activity, and...

journal_title：Molecular and cellular biology

authors： Kontos CD,Cha EH,York JD,Peters KG

更新日期：2002-03-01 00:00:00

abstract：:CD8+ T-cell apoptosis is essential for the contraction phase of the immune response, yet the initiating signals and precise pathways involved are unresolved. The ST3Gal-I sialyltransferase is a candidate mechanistic component and catalyzes sialic acid addition to core 1 O-glycans during protein O glycosylation. ST3Gal...

journal_title：Molecular and cellular biology

authors： Van Dyken SJ,Green RS,Marth JD

更新日期：2007-02-01 00:00:00

abstract：:When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (HCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result fr...

journal_title：Molecular and cellular biology

authors： Wilson AC,Boutros M,Johnson KM,Herr W

更新日期：2000-09-01 00:00:00

abstract：:We report our molecular characterization of the Drosophila melanogaster Abelson gene (abl), a gene in which recessive loss-of-function mutations result in lethality at the pupal stage of development. This essential gene consists of 10 exons extending over 26 kilobase pairs of genomic DNA. The DNA sequence encodes a pr...

journal_title：Molecular and cellular biology

authors： Henkemeyer MJ,Bennett RL,Gertler FB,Hoffmann FM

更新日期：1988-02-01 00:00:00

abstract：:We determined the sequence of a Drosophila tRNA gene cluster containing a tRNAHis gene and a tRNAHis pseudogene in close proximity on the same DNA strand. The pseudogene contains eight consecutive base pairs different from the region of the bona fide gene which codes for the 3' portion of the anticodon stem of tRNAHis...

journal_title：Molecular and cellular biology

authors： Cooley L,Schaack J,Burke DJ,Thomas B,Söll D

更新日期：1984-12-01 00:00:00

abstract：:A gene encoding a novel CACCC box-binding protein that binds to the promoter region of the human T-cell receptor (TCR) V beta 8.1 gene and the mouse TCR alpha gene silencer has been cloned. This gene, termed ht beta, contains four zinc fingers of the class Cys2-X12-His2 that may be responsible for DNA binding and a hi...

journal_title：Molecular and cellular biology

authors： Wang Y,Kobori JA,Hood L

更新日期：1993-09-01 00:00:00

abstract：:Copper is an essential cofactor of two mitochondrial enzymes: cytochrome c oxidase (COX) and Cu-Zn superoxide dismutase (Sod1p). Copper incorporation into these enzymes is facilitated by metallochaperone proteins which probably use copper from a mitochondrial matrix-localized pool. Here we describe a novel conserved m...

journal_title：Molecular and cellular biology

authors： Horn D,Al-Ali H,Barrientos A

更新日期：2008-07-01 00:00:00

abstract：:The Src homology 2-containing phosphotyrosine phosphatase (SHP2) is primarily a positive effector of receptor tyrosine kinase signaling. However, the molecular mechanism by which SHP2 effects its biological function is unknown. In this report, we provide evidence that defines the molecular mechanism and site of action...

journal_title：Molecular and cellular biology

authors： Agazie YM,Hayman MJ

更新日期：2003-11-01 00:00:00

abstract：:We have investigated the relationship between Xenopus laevis c-mos (mosXe) and the cyclin B component of maturation-promoting factor. Microinjection of Xenopus oocytes with in vitro-synthesized RNAs encoding Xenopus cyclin B1 or cyclin B2 induces the progression of meiosis, characterized by germinal vesicle breakdown ...

journal_title：Molecular and cellular biology

authors： Freeman RS,Ballantyne SM,Donoghue DJ

更新日期：1991-03-01 00:00:00

abstract：:In order to create an extended map of chromatin features within a mammalian multigene locus, we have determined the extent of nuclease sensitivity and the pattern of histone modifications associated with the mouse beta-globin genes in adult erythroid tissue. We show that the nuclease-sensitive domain encompasses the b...

journal_title：Molecular and cellular biology

authors： Bulger M,Schübeler D,Bender MA,Hamilton J,Farrell CM,Hardison RC,Groudine M

更新日期：2003-08-01 00:00:00

abstract：:Growth factor independence 1B (GFI1B) coordinates assembly of transcriptional repressor complexes comprised of corepressors and histone-modifying enzymes to control gene expression programs governing lineage allocation in hematopoiesis. Enforced expression of GFI1B in K562 erythroleukemia cells favors erythroid over m...

journal_title：Molecular and cellular biology

authors： McClellan D,Casey MJ,Bareyan D,Lucente H,Ours C,Velinder M,Singer J,Lone MD,Sun W,Coria Y,Mason CC,Engel ME

更新日期：2019-06-13 00:00:00

abstract：:The nuclear receptor peroxisome proliferator-activated receptor gamma regulates adipose differentiation and systemic insulin signaling via ligand-dependent transcriptional activation of target genes. However, the identities of the biologically relevant target genes are largely unknown. Here we describe the isolation a...

journal_title：Molecular and cellular biology

authors： Yoon JC,Chickering TW,Rosen ED,Dussault B,Qin Y,Soukas A,Friedman JM,Holmes WE,Spiegelman BM

更新日期：2000-07-01 00:00:00

abstract：:Poly(A)-binding protein (PABP) stimulates translation initiation by binding simultaneously to the mRNA poly(A) tail and eukaryotic translation initiation factor 4G (eIF4G). PABP activity is regulated by PABP-interacting (Paip) proteins. Paip1 binds PABP and stimulates translation by an unknown mechanism. Here, we desc...

journal_title：Molecular and cellular biology

authors： Martineau Y,Derry MC,Wang X,Yanagiya A,Berlanga JJ,Shyu AB,Imataka H,Gehring K,Sonenberg N

更新日期：2008-11-01 00:00:00

abstract：:To study DNA topological requirements for homologous recombination in plants, we have constructed pairs of plasmids that contain nonoverlapping deletions in the neomycin phosphotransferase gene [APH(3')II], which, when intact, confers kanamycin resistance to plant cells. Protoplasts isolated from Nicotiana tabacum wer...

journal_title：Molecular and cellular biology

authors： Baur M,Potrykus I,Paszkowski J

更新日期：1990-02-01 00:00:00

abstract：:To define the sequence elements involved in initiation of DNA synthesis at the simian virus 40 origin of replication, we determined the relative replication efficiencies in vitro and in vivo of templates containing a variety of mutations within the origin region. Replication of the mutants in vitro was assayed by the ...

journal_title：Molecular and cellular biology

authors： Li JJ,Peden KW,Dixon RA,Kelly T

更新日期：1986-04-01 00:00:00

abstract：:Calpains regulate a wide spectrum of biological functions, including migration, adhesion, apoptosis, secretion, and autophagy, through the modulating cleavage of specific substrates. Ubiquitous microcalpain (μ-calpain) and millicalpain (m-calpain) are heterodimers composed of catalytic subunits encoded, respectively, ...

journal_title：Molecular and cellular biology

authors： Cataldo F,Peche LY,Klaric E,Brancolini C,Myers MP,Demarchi F,Schneider C

更新日期：2013-06-01 00:00:00

abstract：:The SF-21 insect cell line undergoes rapid and widespread apoptosis when treated with actinomycin D or when infected with a mutant of the baculovirus Autographa californica nuclear polyhedrosis virus lacking a p35 gene or a functionally active iap (inhibitor of apoptosis) gene. Here we provide evidence that the basis ...

journal_title：Molecular and cellular biology

authors： Clem RJ,Miller LK

更新日期：1994-08-01 00:00:00