DNA sequence, structure, and tyrosine kinase activity of the Drosophila melanogaster Abelson proto-oncogene homolog.

abstract：:p21Sdi1/WAF1/Cip1 inhibits cyclin-dependent protein kinases and cell proliferation. p21 is presumed to inhibit growth by preventing the phosphorylation of growth-regulatory proteins, including the retinoblastoma tumor suppressor protein (pRb). The ultimate effector(s) of p21 growth inhibition, however, is largely a ma...

journal_title：Molecular and cellular biology

authors： Dimri GP,Nakanishi M,Desprez PY,Smith JR,Campisi J

更新日期：1996-06-01 00:00:00

abstract：:Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox-active cofactor in many biological processes, including DNA replication and repair. Eukaryotic ribonucleotide reductases (RNRs) are Fe-dependent enzymes that catalyze deoxyribonucleoside diphosphate (dNDP) synthesis. We ...

journal_title：Molecular and cellular biology

authors： Sanvisens N,Romero AM,An X,Zhang C,de Llanos R,Martínez-Pastor MT,Bañó MC,Huang M,Puig S

更新日期：2014-09-01 00:00:00

abstract：:Human MED26 was originally purified in the cofactor required for the Sp1 activation complex (CRSP) as a 70-kDa component named CRSP70. This polypeptide was specific to metazoans and the “small” form of the Mediator complex. We report here that a Drosophila melanogaster homologue of MED26 similarly interacts with other...

journal_title：Molecular and cellular biology

authors： Marr SK,Lis JT,Treisman JE,Marr MT 2nd

更新日期：2014-07-01 00:00:00

abstract：:The adenovirus early-region 1B 19,000-molecular-weight tumor antigen is required for oncogenic transformation of cells by adenovirus. We have demonstrated that this tumor antigen is located in the nuclear envelope of infected and transformed cells and that a fraction of the protein within the nuclear envelope is assoc...

journal_title：Molecular and cellular biology

authors： White E,Blose SH,Stillman BW

更新日期：1984-12-01 00:00:00

abstract：:Yju2 is an essential splicing factor required for the first catalytic step after the action of Prp2. We dissected the structure of Yju2 and found that the amino (Yju2-N) and carboxyl (Yju2-C) halves of the protein can be separated and reconstituted for Yju2 function both in vivo and in vitro. Yju2-N has a weak affinit...

journal_title：Molecular and cellular biology

authors： Chiang TW,Cheng SC

更新日期：2013-05-01 00:00:00

abstract：:We cloned a 12.3-kilobase (kb) endogenous plasmid, Ddp1, found in several wild-type and laboratory strains of Dictyostelium discoideum into pBR322. The cloned plasmids have been used to cotransform D. discoideum cells with B10S, a transformation vector carrying a gene fusion conferring resistance to G418. Whereas B10S...

journal_title：Molecular and cellular biology

authors： Firtel RA,Silan C,Ward TE,Howard P,Metz BA,Nellen W,Jacobson A

更新日期：1985-11-01 00:00:00

abstract：:An electrophoretic mobility shift assay was used to characterize interactions of nuclear proteins with a DNA segment in the enhancer element of the leukemogenic murine retrovirus SL3-3. Mutation of a DNA sequence of the 5'-TGTGG-3' type decreased transcription in vivo specifically in T-lymphocyte cell lines. Extracts ...

journal_title：Molecular and cellular biology

authors： Thornell A,Hallberg B,Grundström T

更新日期：1988-04-01 00:00:00

abstract：:Gene expression programs required for differentiation depend on both DNA-bound transcription factors and surrounding histone modifications. Expression of the basic helix-loop-helix (bHLH) protein NeuroD1 is restricted to endocrine cells in the gastrointestinal (GI) tract, where it is important for endocrine differenti...

journal_title：Molecular and cellular biology

authors： Ray SK,Li HJ,Metzger E,Schüle R,Leiter AB

更新日期：2014-06-01 00:00:00

abstract：:A cell-free nuclear replication system that is S-phase specific, that requires the activity of DNA polymerase alpha, and that is stimulated three- to eightfold by cytoplasmic factors from S-phase cells was used to examine the temporal specificity of chromosomal DNA synthesis in vitro. Temporal specificity of DNA synth...

journal_title：Molecular and cellular biology

authors： Heintz NH,Stillman BW

更新日期：1988-05-01 00:00:00

abstract：:Two members of the heat shock transcription factor (HSF) family, HSF1 and HSF2, both function as transcriptional activators of heat shock gene expression. However, the inducible DNA-binding activities of these two factors are regulated by distinct pathways. HSF1 is activated by heat shock and other forms of stress, wh...

journal_title：Molecular and cellular biology

authors： Sistonen L,Sarge KD,Morimoto RI

更新日期：1994-03-01 00:00:00

abstract：:Phosphorylation of eukaryotic initiation factor 2 alpha (eIF-2 alpha) is typically associated with stress responses and causes a reduction in protein synthesis. However, we found high phosphorylated eIF-2 alpha (eIF-2 alpha[P]) levels in nonstressed pancreata of mice. Administration of glucose stimulated a rapid depho...

journal_title：Molecular and cellular biology

authors： Zhang P,McGrath B,Li S,Frank A,Zambito F,Reinert J,Gannon M,Ma K,McNaughton K,Cavener DR

更新日期：2002-06-01 00:00:00

abstract：:Inactivating mutations within the von Hippel-Lindau (VHL) tumor suppressor gene predispose patients to develop a variety of highly vascularized tumors. pVHL targets alpha subunits of the heterodimeric transcription factor hypoxia-inducible factor (HIF), a critical regulator of energy metabolism, angiogenesis, hematopo...

journal_title：Molecular and cellular biology

authors： Mack FA,Patel JH,Biju MP,Haase VH,Simon MC

更新日期：2005-06-01 00:00:00

abstract：:T antigen (Tag) from simian virus 40 binds specifically to two distinct sites in the viral origin of replication and to single-stranded DNA. Analysis of the protein domain responsible for these activities revealed the following. (i) The C-terminal boundary of the origin-specific and single-strand-specific DNA-binding ...

journal_title：Molecular and cellular biology

authors： McVey D,Strauss M,Gluzman Y

更新日期：1989-12-01 00:00:00

abstract：:Xenopus laevis oogenesis is characterized by an active transcription which ceases abruptly upon maturation. To survey changes in the characteristics of the transcriptional machinery which might contribute to this transcriptional arrest, the phosphorylation status of the RNA polymerase II largest subunit (RPB1 subunit)...

journal_title：Molecular and cellular biology

authors： Bellier S,Dubois MF,Nishida E,Almouzni G,Bensaude O

更新日期：1997-03-01 00:00:00

abstract：:To investigate the role of myogenin in regulating acetylcholine receptor expression in adult muscle, this muscle-specific basic helix-loop-helix transcription factor was overexpressed in transgenic mice by using regulatory elements conferring strong expression confined to differentiated postmitotic muscle fibers. Many...

journal_title：Molecular and cellular biology

authors： Gundersen K,Rabben I,Klocke BJ,Merlie JP

更新日期：1995-12-01 00:00:00

abstract：:Alternative mRNA splicing is an important means of diversifying function in higher eukaryotes. Notably, both NCoR and SMRT corepressors are subject to alternative mRNA splicing, yielding a series of distinct corepressor variants with highly divergent functions. Normal adipogenesis is associated with a switch in corepr...

journal_title：Molecular and cellular biology

authors： Goodson ML,Young BM,Snyder CA,Schroeder AC,Privalsky ML

更新日期：2014-11-15 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (Rb) plays a vital role in regulating mammalian cell cycle progression and inactivation of Rb is necessary for entry into S phase. Rb is inactivated by phosphorylation upon growth factor stimulation of quiescent cells, facilitating the transition from G(1) phase to S phase. ...

journal_title：Molecular and cellular biology

authors： Dasgupta P,Sun J,Wang S,Fusaro G,Betts V,Padmanabhan J,Sebti SM,Chellappan SP

更新日期：2004-11-01 00:00:00

abstract：:The GCM family of transcription factors consists of Drosophila melanogaster GCM, an important regulator of gliogenesis in the fly, and its two mammalian homologs, GCMa and GCMb. To clarify the function of these mammalian homologs, we deleted GCMa in mice. Genetic ablation of murine GCMa (mGCMa) is embryonic lethal, wi...

journal_title：Molecular and cellular biology

authors： Schreiber J,Riethmacher-Sonnenberg E,Riethmacher D,Tuerk EE,Enderich J,Bösl MR,Wegner M

更新日期：2000-04-01 00:00:00

abstract：:To define the sequence elements involved in initiation of DNA synthesis at the simian virus 40 origin of replication, we determined the relative replication efficiencies in vitro and in vivo of templates containing a variety of mutations within the origin region. Replication of the mutants in vitro was assayed by the ...

journal_title：Molecular and cellular biology

authors： Li JJ,Peden KW,Dixon RA,Kelly T

更新日期：1986-04-01 00:00:00

abstract：:The regulation of telomerase reverse transcriptase (TERT) plays an important role in the proliferative capacity and survival of cells. Here, we report that exogenously as well as endogenously induced oxidative stress leads to translocation of endogenous as well as overexpressed human TERT from the nucleus into the cyt...

journal_title：Molecular and cellular biology

authors： Haendeler J,Hoffmann J,Brandes RP,Zeiher AM,Dimmeler S

更新日期：2003-07-01 00:00:00

abstract：:The product of the c-myc proto-oncogene is an important positive regulator of cell growth and proliferation. Recently, c-Myc has also been demonstrated to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. To further examine Myc-induced apoptosis, we coexpressed the proto-oncoge...

journal_title：Molecular and cellular biology

authors： Wagner AJ,Small MB,Hay N

更新日期：1993-04-01 00:00:00

abstract：:Neural retina leucine zipper (Nrl), a key basic motif leucine zipper (bZIP) transcription factor, modulates rod photoreceptor differentiation by activating rod-specific target genes. In searching for factors that might couple with Nrl to modulate its transcriptional activity through posttranslational modification, we ...

journal_title：Molecular and cellular biology

authors： Kim JW,Jang SM,Kim CH,An JH,Choi KH

更新日期：2012-05-01 00:00:00

abstract：:The stem-loop in the 5' untranslated region (UTR) of collagen α1(I) and α2(I) mRNAs (5'SL) is the key element regulating their stability and translation. Stabilization of collagen mRNAs is the predominant mechanism for high collagen expression in fibrosis. LARP6 binds the 5'SL of α1(I) and α2(I) mRNAs with high affini...

journal_title：Molecular and cellular biology

authors： Challa AA,Stefanovic B

更新日期：2011-09-01 00:00:00

abstract：:We describe the cloning and characterization of tumor necrosis factor receptor (TNF-R)-associated ubiquitous scaffolding and signaling protein (TRUSS), a novel TNF-R1-interacting protein of 90.7 kDa. TRUSS mRNA was ubiquitously expressed in mouse tissues but was enriched in heart, liver, and testis. Co-immunoprecipita...

journal_title：Molecular and cellular biology

authors： Soond SM,Terry JL,Colbert JD,Riches DW

更新日期：2003-11-01 00:00:00

abstract：:We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridization. To isolate a rec...

journal_title：Molecular and cellular biology

authors： Milbrandt JD,Azizkhan JC,Greisen KS,Hamlin JL

更新日期：1983-07-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the protein phosphatase type 1 (PP1)-binding protein Reg1 is required to maintain complete repression of ADH2 expression during growth on glucose. Surprisingly, however, mutant forms of the yeast PP1 homologue Glc7, which are unable to repress expression of another glucose-regulated gene, ...

journal_title：Molecular and cellular biology

authors： Dombek KM,Voronkova V,Raney A,Young ET

更新日期：1999-09-01 00:00:00

abstract：:Nuclear respiratory-defective mutants of Saccharomyces cerevisiae have been screened for lesions in the mitochondrial alpha-ketoglutarate dehydrogenase complex. Strains assigned to complementation group G70 were ascertained to be deficient in enzyme activity due to mutations in the KGD1 gene coding for the alpha-ketog...

journal_title：Molecular and cellular biology

authors： Repetto B,Tzagoloff A

更新日期：1989-06-01 00:00:00

abstract：:Low oxygen gradients (hypoxia and anoxia) are important determinants of pathological conditions under which the tissue blood supply is deficient or defective, such as in solid tumors. We have been investigating the relationship between the activation of hypoxia-inducible factor 1 (HIF-1), the primary transcriptional r...

journal_title：Molecular and cellular biology

authors： Laderoute KR,Amin K,Calaoagan JM,Knapp M,Le T,Orduna J,Foretz M,Viollet B

更新日期：2006-07-01 00:00:00

abstract：:With v-yes DNA as the probe, a human cDNA library made from placental RNA was screened under relaxed conditions, and DNA clones derived from a novel genetic locus, termed lyn, were obtained. Nucleotide sequencing revealed that lyn could encode a novel tyrosine kinase that was very similar to mouse T-lymphocyte-specifi...

journal_title：Molecular and cellular biology

authors： Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K

更新日期：1987-01-01 00:00:00

abstract：:Mouse mammary tumor virus (MMTV) has long been implicated in mouse mammary carcinogenesis, and it is now well established that the long terminal repeat (LTR) contains regulatory sequences responsible for glucocorticoid-mediated induction of viral RNA. However, we have demonstrated previously that androgens as well as ...

journal_title：Molecular and cellular biology

authors： Darbre P,Page M,King RJ

更新日期：1986-08-01 00:00:00