Nuclear pre-mRNA decapping and 5' degradation in yeast require the Lsm2-8p complex.

abstract：:In this report, we explored the mechanisms underlying keratinocyte-specific and differentiation-specific gene expression in the skin. We have identified five keratinocyte-specific, open chromatin regions that exist within the 6 kb of 5' upstream regulatory sequence known to faithfully recapitulate the strong endogenou...

journal_title：Molecular and cellular biology

authors： Kaufman CK,Sinha S,Bolotin D,Fan J,Fuchs E

更新日期：2002-06-01 00:00:00

abstract：:Misregulation of NF-kappaB signaling leads to infectious, inflammatory, or autoimmune disorders. IkappaB kinase beta (IKKbeta) is an essential activator of NF-kappaB and is known to phosphorylate the NF-kappaB inhibitor, IkappaBalpha, allowing it to undergo ubiquitin-mediated proteasomal degradation. However, beyond I...

journal_title：Molecular and cellular biology

authors： Hutti JE,Turk BE,Asara JM,Ma A,Cantley LC,Abbott DW

更新日期：2007-11-01 00:00:00

abstract：:Broken chromosomes can be repaired by several homologous recombination mechanisms, including gene conversion and break-induced replication (BIR). In Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) is normally repaired by gene conversion. Previously, we have shown that in the absence of R...

journal_title：Molecular and cellular biology

authors： Signon L,Malkova A,Naylor ML,Klein H,Haber JE

更新日期：2001-03-01 00:00:00

abstract：:We report the isolation and molecular characterization of the mouse grb2 gene. The product of this gene, the Grb2 protein, is highly related to the Caenorhabditis elegans sem-5 gene product and the human GRB2 protein and displays the same SH3-SH2-SH3 structural motifs. In situ hybridization studies revealed that the m...

journal_title：Molecular and cellular biology

authors： Suen KL,Bustelo XR,Pawson T,Barbacid M

更新日期：1993-09-01 00:00:00

abstract：:The proto-oncogene c-myb is essential for a controlled balance between cell growth and differentiation. Aberrant c-Myb activity has been reported for numerous human cancers, and enforced c-Myb transcription can transform cells of lymphoid origin by stimulating cellular proliferation and inhibiting apoptotic pathways. ...

journal_title：Molecular and cellular biology

authors： Nicot C,Mahieux R,Pise-Masison C,Brady J,Gessain A,Yamaoka S,Franchini G

更新日期：2001-11-01 00:00:00

abstract：:DHTKD1, a part of 2-ketoadipic acid dehydrogenase complex, is involved in lysine and tryptophan catabolism. Mutations in DHTKD1 block the metabolic pathway and cause 2-aminoadipic and 2-oxoadipic aciduria (AMOXAD), an autosomal recessive inborn metabolic disorder. In addition, a nonsense mutation in DHTKD1 that we ide...

journal_title：Molecular and cellular biology

authors： Xu WY,Zhu H,Shen Y,Wan YH,Tu XD,Wu WT,Tang L,Zhang HX,Lu SY,Jin XL,Fei J,Wang ZG

更新日期：2018-06-14 00:00:00

abstract：:We have identified multiple distinct splicing enhancer elements within protein-coding sequences of the constitutively spliced human beta-globin pre-mRNA. Each of these highly conserved sequences is sufficient to activate the splicing of a heterologous enhancer-dependent pre-mRNA. One of these enhancers is activated by...

journal_title：Molecular and cellular biology

authors： Schaal TD,Maniatis T

更新日期：1999-01-01 00:00:00

abstract：:Preadipocyte factor 1 (Pref-1/Dlk1) inhibits in vitro adipocyte differentiation and has been recently reported to be a paternally expressed imprinted gene at human chromosome 14q32. Studies on human chromosome 14 deletions and maternal uniparental disomy (mUPD) 14 suggest that misexpression of a yet-to-be-identified i...

journal_title：Molecular and cellular biology

authors： Moon YS,Smas CM,Lee K,Villena JA,Kim KH,Yun EJ,Sul HS

更新日期：2002-08-01 00:00:00

abstract：:Dominant and recessive mutations at the SSN20 locus were previously isolated as extragenic suppressors of mutations in three genes (SNF2, SNF5, and SNF6) that are required in trans to derepress invertase expression. All ssn20 alleles cause recessive, temperature-sensitive lethality. In this study we cloned the SSN20 g...

journal_title：Molecular and cellular biology

authors： Neigeborn L,Celenza JL,Carlson M

更新日期：1987-02-01 00:00:00

abstract：:The chimeric oncoprotein E2A-HLF, generated by the t(17;19) chromosomal translocation in pro-B-cell acute lymphoblastic leukemia, incorporates the transactivation domains of E2A and the basic leucine zipper (bZIP) DNA-binding and protein dimerization domain of HLF (hepatic leukemic factor). The ability of E2A-HLF to p...

journal_title：Molecular and cellular biology

authors： Inukai T,Inaba T,Ikushima S,Look AT

更新日期：1998-10-01 00:00:00

abstract：:Initiation of T-lymphocyte-mediated immune responses involves two cellular processes: entry into the cell cycle (G(0)-->G(1)) for clonal proliferation and coordinated changes in surface and secreted molecules that mediate effector functions. However, a point during G(0)-->G(1) beyond which T cells are committed to ent...

journal_title：Molecular and cellular biology

authors： Lea NC,Orr SJ,Stoeber K,Williams GH,Lam EW,Ibrahim MA,Mufti GJ,Thomas NS

更新日期：2003-04-01 00:00:00

abstract：:Genomic imprinting results in parent-of-origin-dependent monoallelic expression of selected genes. Although their importance in development and physiology is recognized, few imprinted genes have been investigated for their effects on brain function. Gnas is a complex imprinted locus whose gene products are involved in...

journal_title：Molecular and cellular biology

authors： Plagge A,Isles AR,Gordon E,Humby T,Dean W,Gritsch S,Fischer-Colbrie R,Wilkinson LS,Kelsey G

更新日期：2005-04-01 00:00:00

abstract：:Previous studies have identified novel modifications in the core fold domain of histone H2B, but relatively little is known about the function of these putative histone modification sites. We have mutated core modifiable residues that are conserved in Saccharomyces cerevisiae histone H2B and characterized the effects ...

journal_title：Molecular and cellular biology

authors： Kyriss MN,Jin Y,Gallegos IJ,Sanford JA,Wyrick JJ

更新日期：2010-07-01 00:00:00

abstract：:DNA elements with the CC(A/T)6GG, or CArG, motif occur in promoters that are under different regulatory controls. CArG elements from the skeletal actin, c-fos, and myogenin genes were tested for their abilities to confer tissue-specific expression on reporter genes when the individual elements were situated immediatel...

journal_title：Molecular and cellular biology

authors： Santoro IM,Walsh K

更新日期：1991-12-01 00:00:00

abstract：:We describe an interaction between homeodomain-interacting protein kinase 1 (HIPK1) and Daxx, two transcriptional regulators important in transducing growth-regulatory signals. We demonstrate that HIPK1 is ubiquitously expressed in mice and humans and localizes predominantly to the nucleus. Daxx normally resides withi...

journal_title：Molecular and cellular biology

authors： Ecsedy JA,Michaelson JS,Leder P

更新日期：2003-02-01 00:00:00

abstract：:TBC (Tre-2/Bub2/Cdc16) domains are predicted to encode GTPase-activating proteins (GAPs) for Rab family G proteins. While approximately 50 TBC proteins are predicted to exist in humans, little is known about their substrate specificity. Here we show that TRE17 (also called Tre-2 and USP6), a founding member of the TBC...

journal_title：Molecular and cellular biology

authors： Martinu L,Masuda-Robens JM,Robertson SE,Santy LC,Casanova JE,Chou MM

更新日期：2004-11-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a key angiogenic factor expressed under restricted nutrient and oxygen conditions in most solid tumors. The expression of VEGF under hypoxic conditions requires transcription through activated hypoxia-inducible factor 1 (HIF-1), increased mRNA stability, and facilitated tra...

journal_title：Molecular and cellular biology

authors： Vumbaca F,Phoenix KN,Rodriguez-Pinto D,Han DK,Claffey KP

更新日期：2008-01-01 00:00:00

abstract：:The recruitment of corepressors by DNA-bound repressors is likely to be a critical rate-limiting step in the transcriptional regulation of many genes. An excellent paradigm for such an interaction is the association of the basic helix-loop-helix zipper protein Mad1 with the corepressor mSin3A. When bound together, the...

journal_title：Molecular and cellular biology

authors： Cowley SM,Kang RS,Frangioni JV,Yada JJ,DeGrand AM,Radhakrishnan I,Eisenman RN

更新日期：2004-04-01 00:00:00

abstract：:The E1B 19-kilodalton protein (19K protein) is a potent apoptosis inhibitor and the adenovirus homolog of Bcl-2 (E. White, Genes Dev. 10:1-15, 1996). To obtain a better understanding of the biochemical mechanism by which the E1B 19K protein regulates apoptosis, proteins that interact with 19K have been identified; one...

journal_title：Molecular and cellular biology

authors： Han J,Sabbatini P,White E

更新日期：1996-10-01 00:00:00

abstract：:Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase, initially discovered as part of the NPM-ALK fusion protein, resulting from the t(2;5) translocation that is frequently associated with anaplastic large-cell lymphomas. The native ALK protein is normally expressed in the developing and, at a weaker level, ...

journal_title：Molecular and cellular biology

authors： Mourali J,Bénard A,Lourenço FC,Monnet C,Greenland C,Moog-Lutz C,Racaud-Sultan C,Gonzalez-Dunia D,Vigny M,Mehlen P,Delsol G,Allouche M

更新日期：2006-08-01 00:00:00

abstract：:The variety of environmental stresses is probably the major challenge imposed on transcription activators and the transcriptional machinery. To precisely describe the very early genomic response developed by yeast to accommodate a chemical stress, we performed time course analyses of the modifications of the yeast gen...

journal_title：Molecular and cellular biology

authors： Lucau-Danila A,Lelandais G,Kozovska Z,Tanty V,Delaveau T,Devaux F,Jacq C

更新日期：2005-03-01 00:00:00

abstract：:We previously identified an RNA binding protein, CUGBP1, which binds to GCN repeats located within the 5' region of C/EBPbeta mRNAs and regulates translation of C/EBPbeta isoforms. To further investigate the role of RNA binding proteins in the posttranscriptional control of C/EBP proteins, we purified additional RNA b...

journal_title：Molecular and cellular biology

authors： Timchenko LT,Iakova P,Welm AL,Cai ZJ,Timchenko NA

更新日期：2002-10-01 00:00:00

abstract：:Several Epstein-Barr virus (EBV)-negative Burkitt lymphoma-derived cell lines (for example, BL41 and Ramos) are extremely sensitive to genotoxic drugs despite being functionally null for the tumor suppressor p53. They rapidly undergo apoptosis, largely from G(2)/M of the cell cycle. 5-bromo-2'-deoxyuridine labeling ex...

journal_title：Molecular and cellular biology

authors： Wade M,Allday MJ

更新日期：2000-02-01 00:00:00

abstract：:EGL-15 is a fibroblast growth factor receptor in the nematode Caenorhabditis elegans. Components that mediate EGL-15 signaling have been identified via mutations that confer a Clear (Clr) phenotype, indicative of hyperactivity of this pathway, or a suppressor-of-Clr (Soc) phenotype, indicative of reduced pathway activ...

journal_title：Molecular and cellular biology

authors： Schutzman JL,Borland CZ,Newman JC,Robinson MK,Kokel M,Stern MJ

更新日期：2001-12-01 00:00:00

abstract：:The establishment of epigenetic marks, such as methylation on histone tails, is mechanistically linked to RNA polymerase II within active genes. To explore the interplay between these modifications in transcribed noncoding as well as coding sequences, we analyzed epigenetic modification and chromatin structure at high...

journal_title：Molecular and cellular biology

authors： Kim A,Kiefer CM,Dean A

更新日期：2007-02-01 00:00:00

abstract：:We have cloned a human gene encoding the 70,000-dalton heat shock protein (HSP70) from a human genomic library, using the Drosophila HSP70 gene as a heterologous hybridization probe. The human recombinant clone hybridized to a 2.6-kilobase polyadenylated mRNA from HeLa cells exposed to 43 degrees C for 2 h. The 2.6-ki...

journal_title：Molecular and cellular biology

authors： Wu B,Hunt C,Morimoto R

更新日期：1985-02-01 00:00:00

abstract：:Eukaryotic flagella are complex organelles composed of more than 200 polypeptides. Little is known about the regulatory mechanisms governing synthesis of the flagellar protein subunits and their assembly into this complex organelle. The unicellular green alga Chlamydomonas reinhardtii is the premier experimental model...

journal_title：Molecular and cellular biology

authors： Periz G,Keller LR

更新日期：1997-07-01 00:00:00

abstract：:Ribosome biogenesis in eukaryotes is a major cellular activity mobilizing the products of over 200 transcriptionally coregulated genes referred to as the rRNA and ribosome biosynthesis regulon. We investigated the function of an essential, uncharacterized gene of this regulon, renamed RRP36. We show that the Rrp36p pr...

journal_title：Molecular and cellular biology

authors： Gérus M,Bonnart C,Caizergues-Ferrer M,Henry Y,Henras AK

更新日期：2010-03-01 00:00:00

abstract：:Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, most likely mediated through two cis-acting sites located on opposite sides of the locus. We showed that deletion of either of these two cis-acting sites from the chromosome di...

journal_title：Molecular and cellular biology

authors： Mahoney DJ,Broach JR

更新日期：1989-11-01 00:00:00

abstract：:In Blastocladiella emersonii zoospores, a set of proteins was found associated with the ribosomes and free ribonucleoprotein particles distinct from the ribosomes and polyribosomes. These proteins were designated P120, P105, P64, P56, and P42 based on their molecular weights determined by gel electrophoresis. Synthesi...

journal_title：Molecular and cellular biology

authors： Jaworski AJ,Stumhofer P

更新日期：1981-04-01 00:00:00