Abstract:
:We have identified multiple distinct splicing enhancer elements within protein-coding sequences of the constitutively spliced human beta-globin pre-mRNA. Each of these highly conserved sequences is sufficient to activate the splicing of a heterologous enhancer-dependent pre-mRNA. One of these enhancers is activated by and binds to the SR protein SC35, whereas at least two others are activated by the SR protein SF2/ASF. A single base mutation within another enhancer element inactivates the enhancer but does not change the encoded amino acid. Thus, overlapping protein coding and RNA recognition elements may be coselected during evolution. These studies provide the first direct evidence that SR protein-specific splicing enhancers are located within the coding regions of constitutively spliced pre-mRNAs. We propose that these enhancers function as multisite splicing enhancers to specify 3' splice-site selection.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Schaal TD,Maniatis Tdoi
10.1128/mcb.19.1.261subject
Has Abstractpub_date
1999-01-01 00:00:00pages
261-73issue
1eissn
0270-7306issn
1098-5549journal_volume
19pub_type
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