Abstract:
:Spt6 is a highly conserved histone chaperone that interacts directly with both RNA polymerase II and histones to regulate gene expression. To gain a comprehensive understanding of the roles of Spt6, we performed genome-wide analyses of transcription, chromatin structure, and histone modifications in a Schizosaccharomyces pombe spt6 mutant. Our results demonstrate dramatic changes to transcription and chromatin structure in the mutant, including elevated antisense transcripts at >70% of all genes and general loss of the +1 nucleosome. Furthermore, Spt6 is required for marks associated with active transcription, including trimethylation of histone H3 on lysine 4, previously observed in humans but not Saccharomyces cerevisiae, and lysine 36. Taken together, our results indicate that Spt6 is critical for the accuracy of transcription and the integrity of chromatin, likely via its direct interactions with RNA polymerase II and histones.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
DeGennaro CM,Alver BH,Marguerat S,Stepanova E,Davis CP,Bähler J,Park PJ,Winston Fdoi
10.1128/MCB.01068-13subject
Has Abstractpub_date
2013-12-01 00:00:00pages
4779-92issue
24eissn
0270-7306issn
1098-5549pii
MCB.01068-13journal_volume
33pub_type
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