Proteasome-mediated degradation of cotranslationally damaged proteins involves translation elongation factor 1A.

abstract：:Type I interferons (IFNs) stimulate transcription through a latent heterotrimeric transcription factor composed of tyrosine-phosphorylated STAT1 and STAT2 and the DNA binding partner IRF9, with STAT2 contributing a critical transactivation domain. Human RVB1 and RVB2, which are highly conserved AAA(+) ATP binding prot...

journal_title：Molecular and cellular biology

authors： Gnatovskiy L,Mita P,Levy DE

更新日期：2013-10-01 00:00:00

abstract：:The polypyrimidine tract near the 3' splice site is important for pre-mRNA splicing. Using pseudouridine incorporation and in vivo RNA-guided RNA pseudouridylation, we have identified two important uridines in the polypyrimidine tract of adenovirus pre-mRNA. Conversion of either uridine into pseudouridine leads to a s...

journal_title：Molecular and cellular biology

authors： Chen C,Zhao X,Kierzek R,Yu YT

更新日期：2010-09-01 00:00:00

abstract：:Tetraspanins are clustered in specific microdomains (named tetraspanin-enriched microdomains, or TERM) in the plasma membrane and regulate the functions of associated transmembrane receptors, including integrins and receptor tyrosine kinases. We have identified syntenin-1, a PDZ domain-containing protein, as a new com...

journal_title：Molecular and cellular biology

authors： Latysheva N,Muratov G,Rajesh S,Padgett M,Hotchin NA,Overduin M,Berditchevski F

更新日期：2006-10-01 00:00:00

abstract：:The transcriptional corepressor mSin3 is associated with histone deacetylases (HDACs) and is utilized by many DNA-binding transcriptional repressors. We have cloned and characterized a novel mSin3A-binding protein, SAP25. SAP25 binds to the PAH1 domain of mSin3A, associates with the mSin3A-HDAC complex in vivo, and re...

journal_title：Molecular and cellular biology

authors： Shiio Y,Rose DW,Aur R,Donohoe S,Aebersold R,Eisenman RN

更新日期：2006-02-01 00:00:00

abstract：:In Drosophila melanogaster, achaete (ac) and m8 are model basic helix-loop-helix activator (bHLH A) and repressor genes, respectively, that have the opposite cell expression pattern in proneural clusters during Notch signaling. Previous studies have shown that activation of m8 transcription in specific cells within pr...

journal_title：Molecular and cellular biology

authors： Cave JW,Xia L,Caudy M

更新日期：2011-01-01 00:00:00

abstract：:Vascular endothelium (VE), the monolayer of endothelial cells that lines the vascular tree, undergoes damage at the basis of some vascular diseases. Its integrity is maintained by VE-cadherin, an adhesive receptor localized at cell-cell junctions. Here, we show that VE-cadherin is also located at the tip and along fil...

journal_title：Molecular and cellular biology

authors： Almagro S,Durmort C,Chervin-Pétinot A,Heyraud S,Dubois M,Lambert O,Maillefaud C,Hewat E,Schaal JP,Huber P,Gulino-Debrac D

更新日期：2010-04-01 00:00:00

abstract：:Although it is generally believed that the 60- and 81-base-pair (60/81-bp) repeats of the Xenopus laevis ribosomal DNA (rDNA) spacer are position-independent transcriptional enhancers, this has not been shown directly. We have now developed a critical assay which proves that the 60/81-bp repeats do, in fact, stimulate...

journal_title：Molecular and cellular biology

authors： Pape LK,Windle JJ,Mougey EB,Sollner-Webb B

更新日期：1989-11-01 00:00:00

abstract：:We previously purified and characterized functionally the Escherichia coli-expressed product of the human subgroup C adenovirus E1A 13S mRNA (B. Ferguson, N. Jones, J. Richter, and M. Rosenberg, Science 224:1343-1346, 1984; B. Krippl, B. Ferguson, M. Rosenberg, and H. Westphal, Proc. Natl. Acad. Sci. USA 81:6988-6992,...

journal_title：Molecular and cellular biology

authors： Ferguson B,Krippl B,Andrisani O,Jones N,Westphal H,Rosenberg M

更新日期：1985-10-01 00:00:00

abstract：:We analyzed the Burkitt's lymphoma line BL64 in which a reciprocal translocation joins the immunoglobulin kappa light-chain locus on chromosome 2 to the c-myc gene on chromosome 8. The breakpoints on the two marker chromosomes 8+ and 2p- occurred 5' of the Js segment within the conserved nonamer and heptamer recombina...

journal_title：Molecular and cellular biology

authors： Hartl P,Lipp M

更新日期：1987-06-01 00:00:00

abstract：:The elongation of transcription is a highly regulated process that requires negative and positive effectors. By binding the double-stranded stem in the transactivation response (TAR) element, RD protein from the negative transcription elongation factor (NELF) inhibits basal transcription from the long terminal repeat ...

journal_title：Molecular and cellular biology

authors： Fujinaga K,Irwin D,Huang Y,Taube R,Kurosu T,Peterlin BM

更新日期：2004-01-01 00:00:00

abstract：:Up to 70% of human genes are associated with regions of nonmethylated DNA called CpG islands (S. Saxonov, P. Berg, and D. L. Brutlag, Proc. Natl. Acad. Sci. U. S. A. 103:1412-1417, 2006). Usually associated with the 5' end of genes, CpG islands are thought to impact gene expression. We previously demonstrated that the...

journal_title：Molecular and cellular biology

authors： Zhou JC,Blackledge NP,Farcas AM,Klose RJ

更新日期：2012-01-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the CDC25 gene product activates adenylate cyclase through RAS1 and RAS2 gene products. We have recently described the cloning of a DNA fragment which suppresses the cdc25 mutation but not ras1, ras2, or cdc35 mutations. This fragment contains a 5'-truncated open reading frame wh...

journal_title：Molecular and cellular biology

authors： Damak F,Boy-Marcotte E,Le-Roscouet D,Guilbaud R,Jacquet M

更新日期：1991-01-01 00:00:00

abstract：:The carboxyl-terminal domain (CTD) of RNA polymerase II (Pol II) can be phosphorylated at serine 2 (Ser-2) and serine 5 (Ser-5) of the CTD heptad repeat YSPTSPS, and this phosphorylation is important in coupling transcription to RNA processing, including 5' capping, splicing, and polyadenylation. The mammalian endogen...

journal_title：Molecular and cellular biology

authors： Cheng C,Sharp PA

更新日期：2003-03-01 00:00:00

abstract：:The cytosolic heat shock cognate 70-kDa protein (hsc70) is required for efficient import of ornithine transcarbamylase precursor (pOTC) into rat liver mitochondria (K. Terada, K. Ohtsuka, N. Imamoto, Y. Yoneda, and M. Mori, Mol. Cell. Biol. 15:3708-3713, 1995). The requirement of hsc70 for mitochondrial import of vari...

journal_title：Molecular and cellular biology

authors： Terada K,Ueda I,Ohtsuka K,Oda T,Ichiyama A,Mori M

更新日期：1996-11-01 00:00:00

abstract：:Transposons have contributed protein coding sequences to a unexpectedly large number of human genes. Except for the V(D)J recombinase and telomerase, all remain of unknown function. Here we investigate the activity of the human SETMAR protein, a highly expressed fusion between a histone H3 methylase and a mariner fami...

journal_title：Molecular and cellular biology

authors： Liu D,Bischerour J,Siddique A,Buisine N,Bigot Y,Chalmers R

更新日期：2007-02-01 00:00:00

abstract：:ICP4 of herpes simplex virus is responsible for the activation of viral transcription during infection. It also efficiently activates and represses transcription in vitro depending on the promoter context. The contacts made between ICP4 and the cellular proteins that result in activated transcription have not been ide...

journal_title：Molecular and cellular biology

authors： Carrozza MJ,DeLuca NA

更新日期：1996-06-01 00:00:00

abstract：:Transcription of Xenopus laevis mitochondrial DNA (xl-mtDNA) by the mitochondrial RNA polymerase requires a dissociable factor. This factor was purified to near homogeneity and identified as a 40-kDa protein. A second protein implicated in the transcription of mtDNA, the Xenopus homolog of the HMG box protein mtTFA, w...

journal_title：Molecular and cellular biology

authors： Antoshechkin I,Bogenhagen DF

更新日期：1995-12-01 00:00:00

abstract：:C3G, which was identified as a Crk SH3 domain-binding guanine nucleotide-releasing factor, shows sequence similarity to CDC25 and Sos family proteins (S. Tanaka, T. Morishita, Y. Hashimoto, S. Hattori, S. Nakamura, M. Shibuya, K. Matuoka, T. Takenawa, T. Kurata, K. Nagashima, and M. Matsuda, Proc. Natl. Acad. Sci. USA...

journal_title：Molecular and cellular biology

authors： Gotoh T,Hattori S,Nakamura S,Kitayama H,Noda M,Takai Y,Kaibuchi K,Matsui H,Hatase O,Takahashi H

更新日期：1995-12-01 00:00:00

abstract：:The O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins is dynamic and abundant in the nucleus and cytosol. Several transcription factors, including Sp1, have been shown to contain this modification; however, the functional role of O-GlcNAc in these proteins has not been determined. In this paper we descr...

journal_title：Molecular and cellular biology

authors： Roos MD,Su K,Baker JR,Kudlow JE

更新日期：1997-11-01 00:00:00

abstract：:The human glycoprotein IIB (GPIIB) gene is expressed only in megakaryocytes, and its promoter displays cell type specificity. We show that this specificity involved two cis-acting sequences. The first one, located at -55, contains a GATA binding site. Point mutations that abolish protein binding on this site decrease ...

journal_title：Molecular and cellular biology

authors： Lemarchandel V,Ghysdael J,Mignotte V,Rahuel C,Roméo PH

更新日期：1993-01-01 00:00:00

abstract：:Tumor necrosis factor alpha (TNF alpha) is a key regulatory cytokine whose expression is controlled by a complex set of stimuli in a variety of cell types. Previously, we found that the monocyte/macrophage-enriched nuclear transcription factor C/EBPbeta played an important role in the regulation of the TNF alpha gene ...

journal_title：Molecular and cellular biology

authors： Zagariya A,Mungre S,Lovis R,Birrer M,Ness S,Thimmapaya B,Pope R

更新日期：1998-05-01 00:00:00

abstract：:Temporal analysis of DNA replication and histone mRNA accumulation in a hamster fibroblast cell cycle mutant (K12) showed that histone mRNA accumulates periodically during the cell cycle and reaches its highest level in the S phase. The direct correlation between the initiation of DNA synthesis and the accumulation of...

journal_title：Molecular and cellular biology

authors： Artishevsky A,Delegeane AM,Lee AS

更新日期：1984-11-01 00:00:00

abstract：:We have previously proposed that IQGAP1, an effector of Rac1 and Cdc42, negatively regulates cadherin-mediated cell-cell adhesion by interacting with beta-catenin and by causing the dissociation of alpha-catenin from cadherin-beta-catenin-alpha-catenin complexes and that activated Rac1 and Cdc42 positively regulate ca...

journal_title：Molecular and cellular biology

authors： Fukata M,Nakagawa M,Itoh N,Kawajiri A,Yamaga M,Kuroda S,Kaibuchi K

更新日期：2001-03-01 00:00:00

abstract：:The steroid hormone progesterone acts via high-affinity nuclear receptors that interact with specific DNA sequences located near the promoter of the hormone-responsive gene. Recent studies suggested that the hormone-occupied progesterone receptor (PR) mediates gene activation by recruiting a cellular coregulatory fact...

journal_title：Molecular and cellular biology

authors： Xu Y,Klein-Hitpass L,Bagchi MK

更新日期：2000-03-01 00:00:00

abstract：:2-Aminopurine (2-AP) inhibits specific kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2. One of these, PKR, is also involved in signal transduction. We show here that 2-AP selectively inhibits expression of tumor necrosis factor alpha (TNF-alpha) mRNA in primary human lymphoid...

journal_title：Molecular and cellular biology

authors： Jarrous N,Osman F,Kaempfer R

更新日期：1996-06-01 00:00:00

abstract：:To examine the functional role of the interaction between Munc18c and syntaxin 4 in the regulation of GLUT4 translocation in 3T3L1 adipocytes, we assessed the effects of introducing three different peptide fragments (20 to 24 amino acids) of Munc18c from evolutionarily conserved regions of the Sec1 protein family pred...

journal_title：Molecular and cellular biology

authors： Thurmond DC,Kanzaki M,Khan AH,Pessin JE

更新日期：2000-01-01 00:00:00

abstract：:We have undertaken total synthesis of the Saccharomyces cerevisiae a-factor (NH2-YIIKGVFWDPAC[S-farnesyl]-COOCH3) and several Cys-12 analogs to determine the significance of S-farnesylation and carboxy-terminal methyl esterification to the biological activity of this lipopeptide mating pheromone. Replacement of either...

journal_title：Molecular and cellular biology

authors： Marcus S,Caldwell GA,Miller D,Xue CB,Naider F,Becker JM

更新日期：1991-07-01 00:00:00

abstract：:The cyclin-dependent kinase (CDK)-activating kinase (CAK) is involved in cell cycle control, transcription, and DNA repair (E. A. Nigg, Curr. Opin. Cell. Biol. 8:312-317, 1996). However, the mechanisms of how CAK is integrated into these signaling pathways remain unknown. We previously demonstrated that abrogation of ...

journal_title：Molecular and cellular biology

authors： Wu L,Chen P,Shum CH,Chen C,Barsky LW,Weinberg KI,Jong A,Triche TJ

更新日期：2001-01-01 00:00:00

abstract：:We have analyzed the 5'-flanking region of one of the genes coding for the human acute-phase protein, serum amyloid A (SAA). We found that SAA mRNA could be increased fivefold in transfected cells by treatment with phorbol 12-myristate 13-acetate (PMA). To analyze this observation further, we placed a 265-base-pair 5'...

journal_title：Molecular and cellular biology

authors： Edbrooke MR,Burt DW,Cheshire JK,Woo P

更新日期：1989-05-01 00:00:00

abstract：:The Saccharomyces cerevisiae Srs2 protein is involved in DNA repair and recombination. In order to gain better insight into the roles of Srs2, we performed a screen to identify mutations that are synthetically lethal with an srs2 deletion. One of them is a mutated allele of the ULP1 gene that encodes a protease specif...

journal_title：Molecular and cellular biology

authors： Soustelle C,Vernis L,Fréon K,Reynaud-Angelin A,Chanet R,Fabre F,Heude M

更新日期：2004-06-01 00:00:00