RasGRF2, a guanosine nucleotide exchange factor for Ras GTPases, participates in T-cell signaling responses.

abstract：:Cwc25 has previously been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (nineteen complex, or NTC) involved in spliceosome activation. We show here that Cwc25 is neither tightly associated with NTC nor required for spliceosome activation but is required f...

journal_title：Molecular and cellular biology

authors： Chiu YF,Liu YC,Chiang TW,Yeh TC,Tseng CK,Wu NY,Cheng SC

更新日期：2009-11-01 00:00:00

abstract：:The vitamin D receptor (VDR) normally functions as a ligand-dependent transcriptional activator. Here we show that, in the presence of Ets-1, VDR stimulates the prolactin promoter in a ligand-independent manner, behaving as a constitutive activator. Mutations in the AF2 domain abolish vitamin D-dependent transactivati...

journal_title：Molecular and cellular biology

authors： Tolón RM,Castillo AI,Jiménez-Lara AM,Aranda A

更新日期：2000-12-01 00:00:00

abstract：:Wnt/β-catenin signaling is a critical regulator of skeletal physiology. However, previous studies have mainly focused on its roles in osteoblasts, while its specific function in osteoclasts is unknown. This is a clinically important question because neutralizing antibodies against Wnt antagonists are promising new dru...

journal_title：Molecular and cellular biology

authors： Wei W,Zeve D,Suh JM,Wang X,Du Y,Zerwekh JE,Dechow PC,Graff JM,Wan Y

更新日期：2011-12-01 00:00:00

abstract：:Phosphorylation of eukaryotic initiation factor 2α (eIF2α) controls transcriptome-wide changes in mRNA translation in stressed cells. While phosphorylated eIF2α (P-eIF2α) attenuates global protein synthesis, mRNAs encoding stress proteins are more efficiently translated. Two eIF2α phosphatases, containing GADD34 and C...

journal_title：Molecular and cellular biology

authors： Reid DW,Tay AS,Sundaram JR,Lee IC,Chen Q,George SE,Nicchitta CV,Shenolikar S

更新日期：2016-06-15 00:00:00

abstract：:The genes CDC36, CDC37, and CDC39, thought to function in the cell division control process in Saccharomyces cerevisiae, were isolated from a recombinant plasmid library prepared by partial digestion of S. cerevisiae genomic DNA with Sau3A and insertion into the S. cerevisiae-Escherichia coli shuttle vector YRp7. In e...

journal_title：Molecular and cellular biology

authors： Breter HJ,Ferguson J,Peterson TA,Reed SI

更新日期：1983-05-01 00:00:00

abstract：:To determine the types of alterations in gene structure induced by DNA-alkylating agents, we analyzed the restriction enzyme cleavage patterns of adenine phosphoribosyltransferase gene sequences in mutant strains of Chinese hamster ovary cells deficient in this enzyme. Base pair changes as detected by loss of restrict...

journal_title：Molecular and cellular biology

authors： Meuth M,Arrand JE

更新日期：1982-11-01 00:00:00

abstract：:We have measured the frequency of meiotic recombination between marked Ty elements in the Saccharomyces cerevisiae genome. These recombination events were usually nonreciprocal (gene conversions) and sometimes involved nonhomologous chromosomes. The frequency of ectopic gene conversion among Ty elements appeared lower...

journal_title：Molecular and cellular biology

authors： Kupiec M,Petes TD

更新日期：1988-07-01 00:00:00

abstract：:Alpha interferon (IFN-alpha) and IFN-beta are able to interfere with viral infection. They exert a vast array of biologic functions, including growth arrest, cell differentiation, and immune system regulation. This regulation extends from innate immunity to cellular and humoral adaptive immune responses. A strict cont...

journal_title：Molecular and cellular biology

authors： Mesplède T,Island ML,Christeff N,Petek F,Doly J,Navarro S

更新日期：2005-10-01 00:00:00

abstract：:GATA-1, a transcription factor essential for the development of the erythroid lineage, contains two adjacent highly conserved zinc finger motifs. The carboxy-terminal finger is necessary and sufficient for specific binding to the consensus GATA recognition sequence: mutant proteins containing only the amino-terminal f...

journal_title：Molecular and cellular biology

authors： Trainor CD,Omichinski JG,Vandergon TL,Gronenborn AM,Clore GM,Felsenfeld G

更新日期：1996-05-01 00:00:00

abstract：:Activation of PERK following the accumulation of unfolded proteins in the endoplasmic reticulum (ER) promotes translation inhibition and cell cycle arrest. PERK function is essential for cell survival following exposure of cells to ER stress, but the mechanisms whereby PERK signaling promotes cell survival are not tho...

journal_title：Molecular and cellular biology

authors： Cullinan SB,Zhang D,Hannink M,Arvisais E,Kaufman RJ,Diehl JA

更新日期：2003-10-01 00:00:00

abstract：:The small subunit of ribonucleotide reductase in Saccharomyces cerevisiae (RNR2) was induced 3- to 20-fold by a variety of DNA-damaging agents. Induction of the RNR2 transcript by at least one of these agents, methyl methanesulfonate, did not require protein synthesis. To identify sequences involved in the regulation ...

journal_title：Molecular and cellular biology

authors： Hurd HK,Roberts JW

更新日期：1989-12-01 00:00:00

abstract：:Two peroxins of the AAA family, PpPex1p and PpPex6p, are required for peroxisome biogenesis in the yeast Pichia pastoris. Cells from the corresponding deletion strains (Pp delta pex1 and Pp delta pex6) contain only small vesicular remnants of peroxisomes, the bulk of peroxisomal matrix proteins is mislocalized to the ...

journal_title：Molecular and cellular biology

authors： Faber KN,Heyman JA,Subramani S

更新日期：1998-02-01 00:00:00

abstract：:Cytosine methylation at CpG dinucleotides is thought to cause more than one-third of all transition mutations responsible for human genetic diseases and cancer. We investigated the methylation status of the CpG dinucleotide at codon 248 in exon 7 of the p53 gene because this codon is a hot spot for inactivating mutati...

journal_title：Molecular and cellular biology

authors： Magewu AN,Jones PA

更新日期：1994-06-01 00:00:00

abstract：:At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic progenitor cells. The rate at which the second event occurs is more than 3 orders of magnitude higher in precursor cell lin...

journal_title：Molecular and cellular biology

authors： Laker C,Kluge N,Stocking C,Just U,Franz MJ,Ostertag W,DeLamarter JF,Dexter M,Spooncer E

更新日期：1989-12-01 00:00:00

abstract：:We have characterized the role of YPR128cp, the orthologue of human PMP34, in fatty acid metabolism and peroxisomal proliferation in Saccharomyces cerevisiae. YPR128cp belongs to the mitochondrial carrier family (MCF) of solute transporters and is localized in the peroxisomal membrane. Disruption of the YPR128c gene r...

journal_title：Molecular and cellular biology

authors： van Roermund CW,Drissen R,van Den Berg M,Ijlst L,Hettema EH,Tabak HF,Waterham HR,Wanders RJ

更新日期：2001-07-01 00:00:00

abstract：:The muscle LIM protein (MLP) and cofilin 2 (CFL2) are important regulators of striated myocyte function. Mutations in the corresponding genes have been directly associated with severe human cardiac and skeletal myopathies, and aberrant expression patterns have often been observed in affected muscles. Herein, we have i...

journal_title：Molecular and cellular biology

authors： Papalouka V,Arvanitis DA,Vafiadaki E,Mavroidis M,Papadodima SA,Spiliopoulou CA,Kremastinos DT,Kranias EG,Sanoudou D

更新日期：2009-11-01 00:00:00

abstract：:Circadian clocks keep time via gene expression feedback loops that are controlled by time-of-day-specific changes in the synthesis, activity, and degradation of transcription factors. Within the Drosophila melanogaster circadian clock, DOUBLETIME (DBT) kinase is necessary for the phosphorylation of PERIOD (PER), a tra...

journal_title：Molecular and cellular biology

authors： Yu W,Zheng H,Price JL,Hardin PE

更新日期：2009-03-01 00:00:00

abstract：:Methylation-controlled J protein (MCJ) is a newly identified member of the DnaJ family of cochaperones. Hypermethylation-mediated transcriptional silencing of the MCJ gene has been associated with increased chemotherapeutic resistance in ovarian cancer. However, the biology and function of MCJ remain unknown. Here we ...

journal_title：Molecular and cellular biology

authors： Hatle KM,Neveu W,Dienz O,Rymarchyk S,Barrantes R,Hale S,Farley N,Lounsbury KM,Bond JP,Taatjes D,Rincón M

更新日期：2007-04-01 00:00:00

abstract：:The use of mutant mice plays a pivotal role in determining the function of genes, and the recently reported germ line transposition of the Sleeping Beauty (SB) transposon would provide a novel system to facilitate this approach. In this study, we characterized SB transposition in the mouse germ line and assessed its p...

journal_title：Molecular and cellular biology

authors： Horie K,Yusa K,Yae K,Odajima J,Fischer SE,Keng VW,Hayakawa T,Mizuno S,Kondoh G,Ijiri T,Matsuda Y,Plasterk RH,Takeda J

更新日期：2003-12-01 00:00:00

abstract：:The signal transducer and activator of transcription STAT5 plays a major role in the cellular response to cytokines, but the mechanism by which it activates transcription remains poorly understood. We show here that deacetylase inhibitors (trichostatin A, suberoylanilide hydroxamic acid, and sodium butyrate) prevent i...

journal_title：Molecular and cellular biology

authors： Rascle A,Johnston JA,Amati B

更新日期：2003-06-01 00:00:00

abstract：:Thyroid hormone receptor (TR) functions as part of multiprotein complexes that also include retinoid X receptor (RXR) and transcriptional coregulators. We have found that both the TR CoR box and ninth heptad are required for RXR interaction and in turn for interaction with corepressor proteins N-CoR and SMRT. Remarkab...

journal_title：Molecular and cellular biology

authors： Zhang J,Zamir I,Lazar MA

更新日期：1997-12-01 00:00:00

abstract：:DET1 (de-etiolated 1) is an essential negative regulator of plant light responses, and it is a component of the Arabidopsis thaliana CDD complex containing DDB1 and COP10 ubiquitin E2 variant. Human DET1 has recently been isolated as one of the DDB1- and Cul4A-associated factors, along with an array of WD40-containing...

journal_title：Molecular and cellular biology

authors： Pick E,Lau OS,Tsuge T,Menon S,Tong Y,Dohmae N,Plafker SM,Deng XW,Wei N

更新日期：2007-07-01 00:00:00

abstract：:Multidrug resistance (MDR) to different cytotoxic compounds in the yeast Saccharomyces cerevisiae can arise from overexpression of the Pdr5 (Sts1, Ydr1, or Lem1) ATP-binding cassette (ABC) multidrug transporter. We have raised polyclonal antibodies recognizing the yeast Pdr5 ABC transporter to study its biogenesis and...

journal_title：Molecular and cellular biology

authors： Egner R,Mahé Y,Pandjaitan R,Kuchler K

更新日期：1995-11-01 00:00:00

abstract：:A novel small nuclear ribonucleoprotein (snRNP) complex containing both U11 and U12 RNAs has been identified in HeLa cell extracts. This U11/U12 snRNP complex can be visualized on glycerol gradients, on native polyacrylamide gels, and by selection with antisense 2'-O-methyl oligoribonucleotides. RNase H-mediated degra...

journal_title：Molecular and cellular biology

authors： Wassarman KM,Steitz JA

更新日期：1992-03-01 00:00:00

abstract：:The expression of the chicken delta 1-crystallin gene is primarily regulated by the action of a lens-specific enhancer 1 kilobase long and located in the third intron of the gene (S. Hayashi, K. Goto, T. S. Okada, and H. Kondoh, Genes Dev. 1:818-828, 1987). The 120-base-long core segment is required for the activity o...

journal_title：Molecular and cellular biology

authors： Goto K,Okada TS,Kondoh H

更新日期：1990-03-01 00:00:00

abstract：:The interaction of the retinoblastoma susceptibility gene product (Rb)-related p107 protein with the E2F transcription factor in S-phase cells facilitates the formation of a multicomponent complex also containing cyclin A and the p33cdk2 kinase. We have created a series of p107 mutants to assess the ability of p107 to...

journal_title：Molecular and cellular biology

authors： Smith EJ,Nevins JR

更新日期：1995-01-01 00:00:00

abstract：:Autophagy is considered an adaptive mechanism against hyperosmotic stress. Although the process has been reported to be triggered by the inhibition of mTORC1, the precise downstream mechanisms remain elusive. Here, we demonstrate that hyperosmotic-stress-induced autophagy is different from conventional macroautophagy ...

journal_title：Molecular and cellular biology

authors： Tamura N,Kageyama S,Komatsu M,Waguri S

更新日期：2019-07-29 00:00:00

abstract：:Genetic and biochemical experiments have enabled us to more clearly distinguish three genetic loci, emtA, emtB, and emtC, all of which can be altered to give rise to resistance to the protein synthesis inhibitor, emetine, in cultured Chinese hamster cells. Genetic experiments have demonstrated that, unlike the emtB lo...

journal_title：Molecular and cellular biology

authors： Chang S,Wasmuth JJ

更新日期：1983-03-01 00:00:00

abstract：:We cloned a 12.3-kilobase (kb) endogenous plasmid, Ddp1, found in several wild-type and laboratory strains of Dictyostelium discoideum into pBR322. The cloned plasmids have been used to cotransform D. discoideum cells with B10S, a transformation vector carrying a gene fusion conferring resistance to G418. Whereas B10S...

journal_title：Molecular and cellular biology

authors： Firtel RA,Silan C,Ward TE,Howard P,Metz BA,Nellen W,Jacobson A

更新日期：1985-11-01 00:00:00

abstract：:The Ras-RAF-mitogen-activated protein kinase (Ras-RAF-MAPK) pathway is overactive in many cancers and in some developmental disorders. In one of those disorders, namely, Noonan syndrome, nine activating C-RAF mutations cluster around Ser(259), a regulatory site for inhibition by 14-3-3 proteins. We show that these mut...

journal_title：Molecular and cellular biology

authors： Molzan M,Schumacher B,Ottmann C,Baljuls A,Polzien L,Weyand M,Thiel P,Rose R,Rose M,Kuhenne P,Kaiser M,Rapp UR,Kuhlmann J,Ottmann C

更新日期：2010-10-01 00:00:00