Meiotic recombination between repeated transposable elements in Saccharomyces cerevisiae.

abstract：:Little is known about the posttranslational control of the cyclin-dependent protein kinase (CDK) inhibitor p21. We describe here a transient phosphorylation of p21 in the G2/M phase. G2/M-phosphorylated p21 is short-lived relative to hypophosphorylated p21. p21 becomes nuclear during S phase, prior to its phosphorylat...

journal_title：Molecular and cellular biology

authors： Dash BC,El-Deiry WS

更新日期：2005-04-01 00:00:00

abstract：:GIPC is a PDZ protein located on peripheral endosomes that binds to the juxtamembrane region of the TrkA nerve growth factor (NGF) receptor and has been implicated in NGF signaling. We establish here that endogenous GIPC binds to the C terminus of APPL, a Rab5 binding protein, which is a marker for signaling endosomes...

journal_title：Molecular and cellular biology

authors： Varsano T,Dong MQ,Niesman I,Gacula H,Lou X,Ma T,Testa JR,Yates JR 3rd,Farquhar MG

更新日期：2006-12-01 00:00:00

abstract：:In Saccharomyces cerevisiae, COX5a and COX5b encode two distinct forms of cytochrome c oxidase subunit V, Va and Vb, respectively. To determine the relative contribution of COX5a and COX5b to cytochrome c oxidase function, we have disrupted each gene. Cytochrome c oxidase activity levels and respiration rates of strai...

journal_title：Molecular and cellular biology

authors： Trueblood CE,Poyton RO

更新日期：1987-10-01 00:00:00

abstract：:Both cyclin D1 and the transcription factor C/EBPβ are required for mammary epithelial cell differentiation; however, the pathway in which they operate is uncertain. Previous analyses of the patterns of gene expression in human tumors suggested a connection between cyclin D1 overexpression and C/EBPβ, but whether this...

journal_title：Molecular and cellular biology

authors： Liu Q,Boudot A,Ni J,Hennessey T,Beauparlant SL,Rajabi HN,Zahnow C,Ewen ME

更新日期：2014-08-01 00:00:00

abstract：:The Sry-related high-mobility-group box transcription factor Sox9 recruits the redundant L-Sox5 and Sox6 proteins to effect chondrogenesis, but the mode of action of the trio remains unclear. We identify here a highly conserved 359-bp sequence 10 kb upstream of the Agc1 gene for aggrecan, a most essential cartilage pr...

journal_title：Molecular and cellular biology

authors： Han Y,Lefebvre V

更新日期：2008-08-01 00:00:00

abstract：:Although the essential involvement of the progesterone receptor (PR) in female reproductive tissues is firmly established, the coregulators preferentially enlisted by PR to mediate its physiological effects have yet to be fully delineated. To further dissect the roles of members of the steroid receptor coactivator (SR...

journal_title：Molecular and cellular biology

authors： Mukherjee A,Soyal SM,Fernandez-Valdivia R,Gehin M,Chambon P,Demayo FJ,Lydon JP,O'Malley BW

更新日期：2006-09-01 00:00:00

abstract：:Appropriate cell cycle checkpoint control is essential for the maintenance of cell and organismal homeostasis. Members of the Nek (NIMA-related kinase) family of serine/threonine protein kinases have been implicated in the regulation of various aspects of the cell cycle. We explored the cellular functions of Nek10, a ...

journal_title：Molecular and cellular biology

authors： Moniz LS,Stambolic V

更新日期：2011-01-01 00:00:00

abstract：:To investigate the transcriptional program underlying thyroid hormone (T3)-induced cell proliferation, cDNA microarrays were used to survey the temporal expression profiles of 4,400 genes. Of 358 responsive genes identified, 88% had not previously been reported to be transcriptionally or functionally modulated by T3. ...

journal_title：Molecular and cellular biology

authors： Miller LD,Park KS,Guo QM,Alkharouf NW,Malek RL,Lee NH,Liu ET,Cheng SY

更新日期：2001-10-01 00:00:00

abstract：:Mutant strain d48 of Paramecium tetraurelia lacks the gene for antigen A in the macronucleus, whereas this gene is present in the micronucleus. Transfer of macronucleoplasm from the wild type to strain d48 caused d48 to revert to the wild type after autogamy. Transfer of cytoplasm was not as effective as transfer of m...

journal_title：Molecular and cellular biology

authors： Harumoto T

更新日期：1986-10-01 00:00:00

abstract：:Following a proliferative phase of variable duration, most normal somatic cells enter a growth arrest state known as replicative senescence. In addition to telomere shortening, a variety of environmental insults and signaling imbalances can elicit phenotypes closely resembling senescence. We used p53(-/-) and p21(-/-)...

journal_title：Molecular and cellular biology

authors： Wei W,Hemmer RM,Sedivy JM

更新日期：2001-10-01 00:00:00

abstract：:How early- and late-firing origins are selected on eukaryotic chromosomes is largely unknown. Here, we show that Mrc1, a conserved factor required for stabilization of stalled replication forks, selectively binds to the early-firing origins in a manner independent of Cdc45 and Hsk1 kinase in the fission yeast Schizosa...

journal_title：Molecular and cellular biology

authors： Hayano M,Kanoh Y,Matsumoto S,Masai H

更新日期：2011-06-01 00:00:00

abstract：:Regulation of variant surface glycoprotein (VSG) mRNA turnover in Trypanosoma brucei was studied in bloodstream forms, in procyclic cells, and during in vitro transformation of bloodstream forms to procyclic cells by approach-to-equilibrium labeling and pulse-chase experiments. Upon initiation of transformation at 27 ...

journal_title：Molecular and cellular biology

authors： Ehlers B,Czichos J,Overath P

更新日期：1987-03-01 00:00:00

abstract：:Phosphoinositides (PIPs) are present in very small amounts but are essential for cell signaling, morphogenesis, and polarity. By mass spectrometry, we demonstrated that some PIPs with stearic acyl chains were strongly disturbed in a psi1Δ Saccharomyces cerevisiae yeast strain deficient in the specific incorporation of...

journal_title：Molecular and cellular biology

authors： Doignon F,Laquel P,Testet E,Tuphile K,Fouillen L,Bessoule JJ

更新日期：2016-02-16 00:00:00

abstract：:Spinal muscular atrophy (SMA) is an inherited motor neuron disease caused by homozygous loss of the Survival Motor Neuron 1 (SMN1) gene. In the absence of SMN1, inefficient inclusion of exon 7 in transcripts from the nearly identical SMN2 gene results in ubiquitous SMN decrease but selective motor neuron degeneration....

journal_title：Molecular and cellular biology

authors： Ruggiu M,McGovern VL,Lotti F,Saieva L,Li DK,Kariya S,Monani UR,Burghes AH,Pellizzoni L

更新日期：2012-01-01 00:00:00

abstract：:alpha 1-Antitrypsin (AT), the major elastase inhibitor in mammalian serum, is produced primarily in the liver. We have characterized AT gene structure and expression in the mouse species Mus caroli, which expresses high levels of AT in the kidneys as well as in the liver. Analysis of cDNA and genomic clones showed tha...

journal_title：Molecular and cellular biology

authors： Latimer JJ,Berger FG,Baumann H

更新日期：1990-02-01 00:00:00

abstract：:Densely methylated DNA sequence islands, designated DMIs, have been observed in two Chinese hamster cell chromosomal replication origins by using a PCR-based chemical method of detection. One of the origins, oriS14, is located within or adjacent to the coding sequence for ribosomal protein S14 on chromosome 2q, and th...

journal_title：Molecular and cellular biology

authors： Tasheva ES,Roufa DJ

更新日期：1994-09-01 00:00:00

abstract：:The two beta-tubulin genes of the unicellular green alga Chlamydomonas reinhardtii are expressed coordinately after deflagellation and produce two transcripts of 2.1 and 2.0 kilobases. Full-length cDNA clones corresponding to the transcript of each gene were isolated. DNA sequences were obtained from the cDNA clones a...

journal_title：Molecular and cellular biology

authors： Youngblom J,Schloss JA,Silflow CD

更新日期：1984-12-01 00:00:00

abstract：:We isolated the promoter of the human gene encoding the 94,000-dalton glucose-regulated protein (GRP94). The 5'-flanking region important for its expression was identified by deletion analysis. Comparison of the promoters of the genes for GRP78 and GRP94 derived from human, rat, and chicken cells revealed a common dom...

journal_title：Molecular and cellular biology

authors： Chang SC,Erwin AE,Lee AS

更新日期：1989-05-01 00:00:00

abstract：:The Saccharomyces cerevisiae PGK (phosphoglycerate kinase) gene encodes one of the most abundant mRNA and protein species in the cell. To identify the promoter sequences required for the efficient expression of PGK, we undertook a detailed internal deletion analysis of the 5' noncoding region of the gene. Our analysis...

journal_title：Molecular and cellular biology

authors： Ogden JE,Stanway C,Kim S,Mellor J,Kingsman AJ,Kingsman SM

更新日期：1986-12-01 00:00:00

abstract：:The Mauriceville retroplasmid of Neurospora mitochondria encodes a novel reverse transcriptase that initiates cDNA synthesis de novo (i.e., without a primer) at the 3' CCA of the plasmid transcript's 3' tRNA-like structure (H. Wang and A. M. Lambowitz, Cell 75:1071-1081, 1993). Here, we show that the plasmid reverse t...

journal_title：Molecular and cellular biology

authors： Chiang CC,Lambowitz AM

更新日期：1997-08-01 00:00:00

abstract：:The distal region (-830 to -720 bp) of the rat whey acidic protein (WAP) gene contains a composite response element (CoRE), which has been demonstrated previously to confer mammary gland-specific and hormonally regulated WAP gene expression. Point mutations in the binding sites for specific transcription factors prese...

journal_title：Molecular and cellular biology

authors： Mukhopadhyay SS,Wyszomierski SL,Gronostajski RM,Rosen JM

更新日期：2001-10-01 00:00:00

abstract：:By immunoblotting with antibodies for phosphotyrosine, we have demonstrated that the hematopoietic growth factors interleukin-2, interleukin-3, interleukin-4, and granulocyte-macrophage colony-stimulating factor stimulate the tyrosine phosphorylation of specific sets of proteins in murine hematopoietic progenitor cell...

journal_title：Molecular and cellular biology

authors： Morla AO,Schreurs J,Miyajima A,Wang JY

更新日期：1988-05-01 00:00:00

abstract：:The SR protein SRp38 is a general splicing repressor that is activated by dephosphorylation during mitosis and in response to heat shock. Here we describe experiments that provide insights into the mechanism by which SRp38 functions in splicing repression. We first show that SRp38 redistributes and colocalizes with sn...

journal_title：Molecular and cellular biology

authors： Shin C,Kleiman FE,Manley JL

更新日期：2005-09-01 00:00:00

abstract：:Xenopus in vitro studies have implicated both transforming growth factor beta (TGF-beta) and fibroblast growth factor (FGF) families in mesoderm induction. Although members of both families are present during mouse mesoderm formation, there is little evidence for their functional role in mesoderm induction. We show th...

journal_title：Molecular and cellular biology

authors： Johansson BM,Wiles MV

更新日期：1995-01-01 00:00:00

abstract：:RAG1 and RAG2 catalyze the first DNA cleavage steps in V(D)J recombination. We demonstrate that the isolated central domain of RAG1 has inherent single-stranded (ss) DNA cleavage activity, which does not require, but is enhanced by, RAG2. The central domain, therefore, contains the active-site residues necessary to pe...

journal_title：Molecular and cellular biology

authors： De P,Peak MM,Rodgers KK

更新日期：2004-08-01 00:00:00

abstract：:We have cloned CDC9, the structural gene for Saccharomyces cerevisiae DNA ligase, and investigated its transcriptional regulation both as a function of cell cycle stage and after UV irradiation. The steady-state level of DNA ligase mRNA increases at least fourfold in late G1, after the completion of start but before S...

journal_title：Molecular and cellular biology

authors： Peterson TA,Prakash L,Prakash S,Osley MA,Reed SI

更新日期：1985-01-01 00:00:00

abstract：:The full-length normal and T24 mutant human H-ras proteins and two truncated derivatives of the T24 mutant were expressed efficiently in Escherichia coli. The proteins accumulated to 1 to 5% of total cellular protein, and each was specifically recognized by anti-ras monoclonal antibodies. The two full-length proteins ...

journal_title：Molecular and cellular biology

authors： Gross M,Sweet RW,Sathe G,Yokoyama S,Fasano O,Goldfarb M,Wigler M,Rosenberg M

更新日期：1985-05-01 00:00:00

abstract：:Amino acids 106 to 143 and 354 to 433 of the human c-myc protein (439 amino acids) were shown to be required for the protein to suppress c-myc gene transcription and were found to exactly overlap with those necessary for c-myc to cooperate with ras oncogenes in the transformation of rat embryo fibroblasts. The essenti...

journal_title：Molecular and cellular biology

authors： Penn LJ,Brooks MW,Laufer EM,Littlewood TD,Morgenstern JP,Evan GI,Lee WM,Land H

更新日期：1990-09-01 00:00:00

abstract：:To investigate the role of insulin receptor substrate 1 (IRS-1) and IRS-2, the two ubiquitously expressed IRS proteins, in adipocyte differentiation, we established embryonic fibroblast cells with four different genotypes, i.e., wild-type, IRS-1 deficient (IRS-1(-/-)), IRS-2 deficient (IRS-2(-/-)), and IRS-1 IRS-2 dou...

journal_title：Molecular and cellular biology

authors： Miki H,Yamauchi T,Suzuki R,Komeda K,Tsuchida A,Kubota N,Terauchi Y,Kamon J,Kaburagi Y,Matsui J,Akanuma Y,Nagai R,Kimura S,Tobe K,Kadowaki T

更新日期：2001-04-01 00:00:00

abstract：:Recombinant type 1 transforming growth factor beta (TGF-beta) was expressed to high levels in CHO cells by using dihydrofolate reductase (dhfr) gene amplification. The expression plasmid was derived from the pSV2 vectors and contained, in tandem, the simian TGF-beta and mouse dhfr cDNAs. Transcription of both cDNAs wa...

journal_title：Molecular and cellular biology

authors： Gentry LE,Webb NR,Lim GJ,Brunner AM,Ranchalis JE,Twardzik DR,Lioubin MN,Marquardt H,Purchio AF

更新日期：1987-10-01 00:00:00