Abstract:
:U12-dependent introns containing alterations of the 3' splice site AC dinucleotide or alterations in the spacing between the branch site and the 3' splice site were examined for their effects on splice site selection in vivo and in vitro. Using an intron with a 5' splice site AU dinucleotide, any nucleotide could serve as the 3'-terminal nucleotide, although a C residue was most active, while a U residue was least active. The penultimate A residue, by contrast, was essential for 3' splice site function. A branch site-to-3' splice site spacing of less than 10 or more than 20 nucleotides strongly activated alternative 3' splice sites. A strong preference for a spacing of about 12 nucleotides was observed. The combined in vivo and in vitro results suggest that the branch site is recognized in the absence of an active 3' splice site but that formation of the prespliceosomal complex A requires an active 3' splice site. Furthermore, the U12-type spliceosome appears to be unable to scan for a distal 3' splice site.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Dietrich RC,Peris MJ,Seyboldt AS,Padgett RAdoi
10.1128/MCB.21.6.1942-1952.2001subject
Has Abstractpub_date
2001-03-01 00:00:00pages
1942-52issue
6eissn
0270-7306issn
1098-5549journal_volume
21pub_type
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