Abstract:
:Morphological development of the fungal pathogen Candida albicans is profoundly affected by ambient pH. Acidic pH restricts growth to the yeast form, whereas neutral pH permits development of the filamentous form. Superimposed on the pH restriction is a temperature requirement of approximately 37 degrees C for filamentation. The role of pH in development was investigated by selecting revertants of phr2Delta mutants that had gained the ability to grow at acid pH. The extragenic suppressors in two independent revertants were identified as nonsense mutations in the pH response regulator RIM101 (PRR2) that resulted in a carboxy-terminal truncation of the open reading frame. These dominant active alleles conferred the ability to filament at acidic pH, to express PHR1, an alkaline-expressed gene, at acidic pH, and to repress the acid-expressed gene PHR2. It was also observed that both the wild-type and mutant alleles could act as multicopy suppressors of the temperature restriction on filamentation, allowing extensive filamentation at 29 degrees C. The ability of the activated alleles to promote filamentation was dependent upon the developmental regulator EFG1. The results suggest that RIM101 is responsible for the pH dependence of hyphal development.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
El Barkani A,Kurzai O,Fonzi WA,Ramon A,Porta A,Frosch M,Mühlschlegel FAdoi
10.1128/mcb.20.13.4635-4647.2000subject
Has Abstractpub_date
2000-07-01 00:00:00pages
4635-47issue
13eissn
0270-7306issn
1098-5549journal_volume
20pub_type
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