Abstract:
:In Saccharomyces cerevisiae, the GTP-binding Ypt1 protein (Ypt1p) is essential for endoplasmic reticulum-to-Golgi protein transport. By exploiting a GAL10-YPT1 fusion to regulate YPT1 expression, three multicopy suppressors, SLY2, SLY12, and SLY41, and a single-copy suppressor, SLY1-20, that allowed YPT1-independent growth were isolated. Wild-type Sly1p is hydrophilic, is essential for cell viability, and differs from Sly1-20p by a single amino acid. SLY2 and SLY12 encode proteins with hydrophobic tails similar to synaptobrevins, integral membrane proteins of synaptic vesicles in higher eucaryotes. Sly41p is hydrophobic and exhibits sequence similarities with the chloroplast phosphate translocator. SLY12 but not SLY41 is an essential gene. The SLY2 null mutant is cold and heat sensitive. The SLY gene products may comprise elements of the protein transport machinery.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Dascher C,Ossig R,Gallwitz D,Schmitt HDdoi
10.1128/mcb.11.2.872subject
Has Abstractpub_date
1991-02-01 00:00:00pages
872-85issue
2eissn
0270-7306issn
1098-5549journal_volume
11pub_type
杂志文章abstract::We characterized the organization and expression of PHO5 and PHO3, the tightly linked repressible and constitutive acid phosphatase genes of Saccharomyces cerevisiae. The "constitutive" gene, PHO3, is expressed only when PHO5 is not. Altering PHO5 expression, either through promoter deletions or through mutations in t...
journal_title:Molecular and cellular biology
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journal_title:Molecular and cellular biology
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pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/mcb.7.11.4100
更新日期:1987-11-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.9.5725
更新日期:1993-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1984-06-01 00:00:00
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更新日期:2005-05-01 00:00:00
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journal_title:Molecular and cellular biology
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.11.3.1713
更新日期:1991-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1990-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-09-01 00:00:00