Phosphorylation of SAF-A/hnRNP-U Serine 59 by Polo-Like Kinase 1 Is Required for Mitosis.

abstract：:Nicotine adenine dinucleotide (NAD(+)) performs key roles in electron transport reactions, as a substrate for poly(ADP-ribose) polymerase and NAD(+)-dependent protein deacetylases. In the latter two processes, NAD(+) is consumed and converted to ADP-ribose and nicotinamide. NAD(+) levels can be maintained by regenerat...

journal_title：Molecular and cellular biology

authors： Bedalov A,Hirao M,Posakony J,Nelson M,Simon JA

更新日期：2003-10-01 00:00:00

abstract：:Rad9 is required for the activation of DNA damage checkpoint pathways in budding yeast. Rad9 is phosphorylated after DNA damage in a Mec1- and Tel1-dependent manner and subsequently interacts with Rad53. This Rad9-Rad53 interaction has been suggested to trigger the activation and phosphorylation of Rad53. Here we show...

journal_title：Molecular and cellular biology

authors： Naiki T,Wakayama T,Nakada D,Matsumoto K,Sugimoto K

更新日期：2004-04-01 00:00:00

abstract：:The homeo box gene even-skipped (eve) encodes a 376-amino-acid protein that binds with high affinity to sequences located near the 5' termini of the eve and en genes. The 5' en sites are A + T rich and contain copies of the 10-base-pair (bp) consensus sequence T-C-A-A-T-T-A-A-A-T. In contrast, the 5' eve sites are G +...

journal_title：Molecular and cellular biology

authors： Hoey T,Warrior R,Manak J,Levine M

更新日期：1988-11-01 00:00:00

abstract：:Saccharomyces cerevisiae contains several prion elements, which are epigenetically transmitted as self-perpetuating protein conformations. One such prion is [SWI + ], whose protein determinant is Swi1, a subunit of the SWI/SNF chromatin-remodeling complex. We previously rep...

journal_title：Molecular and cellular biology

authors： Valtierra S,Du Z,Li L

更新日期：2017-09-26 00:00:00

abstract：:Liu and Mertz (Genes Dev. 9:1766-1780, 1995) previously identified a 119-nt pre-mRNA processing enhancer (PPE) element within the herpes simplex virus type 1 thymidine kinase gene that enables intron-independent gene expression in higher eukaryotes by binding heterogeneous nuclear ribonucleoprotein L (hnRNP L). Here, ...

journal_title：Molecular and cellular biology

authors： Guang S,Felthauser AM,Mertz JE

更新日期：2005-08-01 00:00:00

abstract：:Thrombin stimulation of platelets induces a transient increase in the specific activity of pp60c-src followed by a redistribution of pp60c-src to the Triton X-100-insoluble, cytoskeleton-rich fraction. Concomitant with the observed increase in pp60c-src activity was a rapid dephosphorylation of tyrosine 527 in 10 to 1...

journal_title：Molecular and cellular biology

authors： Clark EA,Brugge JS

更新日期：1993-03-01 00:00:00

abstract：:The inducible transcriptional complex AP-1, composed of c-Fos and c-Jun proteins, is crucial for cell adaptation to many environmental changes. While its mechanisms of activation have been extensively studied, how its activity is restrained is poorly understood. We report here that lysine 265 of c-Fos is conjugated by...

journal_title：Molecular and cellular biology

authors： Bossis G,Malnou CE,Farras R,Andermarcher E,Hipskind R,Rodriguez M,Schmidt D,Muller S,Jariel-Encontre I,Piechaczyk M

更新日期：2005-08-01 00:00:00

abstract：:Although the primary function of U1 snRNA is to define the 5' donor site of an intron, it can also block the accumulation of a specific RNA transcript when it binds to a donor sequence within its terminal exon. This work was initiated to investigate if this property of U1 snRNA could be exploited as an effective metho...

journal_title：Molecular and cellular biology

authors： Beckley SA,Liu P,Stover ML,Gunderson SI,Lichtler AC,Rowe DW

更新日期：2001-04-01 00:00:00

abstract：:Unique single-stranded regions of simian virus 40 DNA, phage M13 virion DNA, and several homopolymers were used as templates for the synthesis of (p)ppRNA-DNA chains by CV-1 cell DNA primase-DNA polymerase alpha. Intact RNA primers, specifically labeled with an RNA capping enzyme, were typically 6 to 8 ribonucleotides...

journal_title：Molecular and cellular biology

authors： Yamaguchi M,Hendrickson EA,DePamphilis ML

更新日期：1985-05-01 00:00:00

abstract：:We report the cloning and functional analysis of a complete clone for the third member of the mouse mdr gene family, mdr3. Nucleotide and predicted amino acid sequence analyses showed that the three mouse mdr genes encode highly homologous membrane glycoproteins, which share the same length (1,276 residues), the same ...

journal_title：Molecular and cellular biology

authors： Devault A,Gros P

更新日期：1990-04-01 00:00:00

abstract：:Previously, we identified the E3 ubiquitin ligase Idol (inducible degrader of the low-density lipoprotein [LDL] receptor [LDLR]) as a posttranscriptional regulator of the LDLR pathway. Idol stimulates LDLR degradation through ubiquitination of its C-terminal domain, thereby limiting cholesterol uptake. Here we report ...

journal_title：Molecular and cellular biology

authors： Scotti E,Hong C,Yoshinaga Y,Tu Y,Hu Y,Zelcer N,Boyadjian R,de Jong PJ,Young SG,Fong LG,Tontonoz P

更新日期：2011-05-01 00:00:00

abstract：:In a gene trap screen for genes expressed in the primitive streak and tail bud during mouse embryogenesis, we isolated a mutation in Jade1, a gene encoding a PHD zinc finger protein previously shown to interact with the tumor suppressor pVHL. Expressed sequence tag analysis indicates that Jade1 is subject to posttrans...

journal_title：Molecular and cellular biology

authors： Tzouanacou E,Tweedie S,Wilson V

更新日期：2003-12-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae CYC1 gene produces mRNA with more than 20 different 5' ends. A derivative of the CYC1 gene (CYC1-157) was constructed with a deletion of a portion of the CYC1 5'-noncoding region, which includes the sites at which many of the CYC1 mRNAs 5' ends map. A 54-mer double-stranded o...

journal_title：Molecular and cellular biology

authors： McNeil JB,Smith M

更新日期：1985-12-01 00:00:00

abstract：:Phosphorylation of eukaryotic initiation factor 2 alpha (eIF-2 alpha) is typically associated with stress responses and causes a reduction in protein synthesis. However, we found high phosphorylated eIF-2 alpha (eIF-2 alpha[P]) levels in nonstressed pancreata of mice. Administration of glucose stimulated a rapid depho...

journal_title：Molecular and cellular biology

authors： Zhang P,McGrath B,Li S,Frank A,Zambito F,Reinert J,Gannon M,Ma K,McNaughton K,Cavener DR

更新日期：2002-06-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the Wee1 family kinase Swe1p is normally stable during G(1) and S phases but is unstable during G(2) and M phases due to ubiquitination and subsequent degradation. However, perturbations of the actin cytoskeleton lead to a stabilization and accumulation of Swe1p. This response constitutes ...

journal_title：Molecular and cellular biology

authors： McMillan JN,Longtine MS,Sia RA,Theesfeld CL,Bardes ES,Pringle JR,Lew DJ

更新日期：1999-10-01 00:00:00

abstract：:The first processing step in the maturation of mouse precursor rRNA involves cleavage at nucleotide ca. +650, at the 5' border of a 200-nucleotide region that is conserved across mammals and contains the sequences that direct the processing. To identify the relevant sequence elements, we used rRNAs with small internal...

journal_title：Molecular and cellular biology

authors： Craig N,Kass S,Sollner-Webb B

更新日期：1991-01-01 00:00:00

abstract：:Saccharomyces cerevisiae mating type switching is a gene conversion event that exhibits donor preference. MATa cells choose HMLalpha for recombination, and MATalpha cells choose HMRa. Donor preference is controlled by the recombination enhancer (RE), located between HMLalpha and MATa on the left arm of chromosome III....

journal_title：Molecular and cellular biology

authors： Ercan S,Reese JC,Workman JL,Simpson RT

更新日期：2005-09-01 00:00:00

abstract：:We have studied the mechanism by which 5'-flanking sequences modulate the in vitro transcription of eucaryotic tRNA genes. Using deletion and linker substitution mutagenesis, we have found that the 5'-flanking sequences responsible for the different in vitro transcription levels of three Drosophila tRNA5Asn genes are ...

journal_title：Molecular and cellular biology

authors： Lofquist AK,Garcia AD,Sharp SJ

更新日期：1988-10-01 00:00:00

abstract：:Tumor-promoting phorbol esters affect a variety of cellular functions which may underlie tumor promotion. We isolated from human lymphocytes a cDNA clone whose gene is inducible by the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate as well as by the T-cell mitogen phytohemagglutinin. Nuclear transcription experim...

journal_title：Molecular and cellular biology

authors： Arya SK,Wong-Staal F,Gallo RC

更新日期：1984-11-01 00:00:00

abstract：:Wnt signaling is thought to be mediated via interactions between beta-catenin and members of the LEF-1/TCF family of transcription factors. Here we study the mechanism of transcriptional regulation by LEF-1 in response to a Wnt-1 signal under conditions of endogenous beta-catenin in NIH 3T3 cells, and we examine wheth...

journal_title：Molecular and cellular biology

authors： Hsu SC,Galceran J,Grosschedl R

更新日期：1998-08-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) Tat protein activates transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1. CDK9 is able to hyperphosphorylate the carboxyl-terminal domain (CTD) of the large subunit of RNA pol...

journal_title：Molecular and cellular biology

authors： Bourgeois CF,Kim YK,Churcher MJ,West MJ,Karn J

更新日期：2002-02-01 00:00:00

abstract：:Precursor RNA containing the adenovirus L3 polyadenylation site is assembled into a 50S complex upon incubation with HeLa nuclear extract at 30 degrees C. The cofactor and sequence requirements for 50S complex formation are similar to those of the in vitro polyadenylation reaction. Assembly of this complex requires AT...

journal_title：Molecular and cellular biology

authors： Moore CL,Skolnik-David H,Sharp PA

更新日期：1988-01-01 00:00:00

abstract：:macroH2A histone variants have been implicated to function in gene silencing by several studies, including ones showing a preferential association of macroH2A on the inactive X chromosome. To examine macroH2A function in vivo, we knocked out macroH2A1. macroH2A1 knockout mice are viable and fertile. A broad screen of ...

journal_title：Molecular and cellular biology

authors： Changolkar LN,Costanzi C,Leu NA,Chen D,McLaughlin KJ,Pehrson JR

更新日期：2007-04-01 00:00:00

abstract：:USF is a family of basic helix-loop transcriptional factors that recognizes DNA-binding sites similar to those of the Myc oncoproteins. Here, various functional domains in the mouse USF2 protein were identified and characterized. Indirect immunofluorescence studies with transiently transfected cells revealed that both...

journal_title：Molecular and cellular biology

authors： Luo X,Sawadogo M

更新日期：1996-04-01 00:00:00

abstract：:Metazoan replication-dependent histone mRNAs are the only eukaryotic mRNAs that are not polyadenylated. The cleavage of histone pre-mRNA to form the unique 3' end requires the U7 snRNP and the stem-loop binding protein (SLBP) that binds the 3' end of histone mRNA. U7 snRNP contains three novel proteins, Lsm10 and Lsm1...

journal_title：Molecular and cellular biology

authors： Wagner EJ,Marzluff WF

更新日期：2006-09-01 00:00:00

abstract：:XPC is responsible for DNA damage sensing in nucleotide excision repair (NER). Mutations in XPC lead to a defect in NER and to xeroderma pigmentosum (XP-C). Here, we analyzed the biochemical properties behind mutations found within three patients: one amino acid substitution (P334H, XP1MI, and GM02096), one amino acid...

journal_title：Molecular and cellular biology

authors： Bernardes de Jesus BM,Bjørås M,Coin F,Egly JM

更新日期：2008-12-01 00:00:00

abstract：:Skp2B, an F-box protein of unknown function, is frequently overexpressed in breast cancer. In order to determine the function of Skp2B and whether it has a role in breast cancer, we performed a two-hybrid screen and established transgenic mice expressing Skp2B in the mammary glands. We found that Skp2B interacts with ...

journal_title：Molecular and cellular biology

authors： Umanskaya K,Radke S,Chander H,Monardo R,Xu X,Pan ZQ,O'Connell MJ,Germain D

更新日期：2007-11-01 00:00:00

abstract：:The Epstein-Barr virus BRLF1 and BZLF1 genes are the first viral genes transcribed upon induction of the viral lytic cycle. The protein products of both genes (referred to here as Rta and Zta, respectively) activate expression of other viral genes, thereby initiating the lytic cascade. Among the viral antigens express...

journal_title：Molecular and cellular biology

authors： Flemington EK,Lytle JP,Cayrol C,Borras AM,Speck SH

更新日期：1994-05-01 00:00:00

abstract：:Ischemia in the heart deprives cardiomyocytes of oxygen, triggering cell death (myocardial infarction). Ischemia and its cell culture model, hypoxia, elicit a stress response program that contributes to cardiomyocyte death; however, the molecular components required to promote this process remain nebulous. Gene 33 is ...

journal_title：Molecular and cellular biology

authors： Xu D,Patten RD,Force T,Kyriakis JM

更新日期：2006-07-01 00:00:00

abstract：:Sirtuins are a family of protein deacetylases, deacylases, and ADP-ribosyltransferases that regulate life span, control the onset of numerous age-associated diseases, and mediate metabolic homeostasis. We have uncovered a novel role for the mitochondrial sirtuin SIRT4 in the regulation of hepatic lipid metabolism duri...

journal_title：Molecular and cellular biology

authors： Laurent G,de Boer VC,Finley LW,Sweeney M,Lu H,Schug TT,Cen Y,Jeong SM,Li X,Sauve AA,Haigis MC

更新日期：2013-11-01 00:00:00