Sequence and spatial requirements for the tissue- and species-independent 3'-end processing mechanism of plant mRNA.

abstract：:Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1) signa...

journal_title：Molecular and cellular biology

authors： Llobet-Navas D,Rodriguez-Barrueco R,de la Iglesia-Vicente J,Olivan M,Castro V,Saucedo-Cuevas L,Marshall N,Putcha P,Castillo-Martin M,Bardot E,Ezhkova E,Iavarone A,Cordon-Cardo C,Silva JM

更新日期：2014-12-01 00:00:00

abstract：:PHAX (phosphorylated adaptor for RNA export) is the key regulator of U snRNA nuclear export in metazoa. Our previous work revealed that PHAX is phosphorylated in the nucleus and is exported as a component of the U snRNA export complex to the cytoplasm, where it is dephosphorylated (M. Ohno, A. Segref, A. Bachi, M. Wil...

journal_title：Molecular and cellular biology

authors： Kitao S,Segref A,Kast J,Wilm M,Mattaj IW,Ohno M

更新日期：2008-01-01 00:00:00

abstract：:The ascomycete Saccharomyces cerevisiae exhibits alternative vegetative growth states referred to as the yeast form and the filamentous form, and it switches between the two morphologies depending on specific environmental signals. To identify molecules involved in control of morphologic differentiation, this study ch...

journal_title：Molecular and cellular biology

authors： Edgington NP,Blacketer MJ,Bierwagen TA,Myers AM

更新日期：1999-02-01 00:00:00

abstract：:A fragment of the mouse mammary tumor virus (MMTV) promoter was reconstituted from pure histones into a dinucleosome with uniquely positioned octamer cores. Core boundaries for the in vitro-assembled dinucleosome corresponded to the observed in vivo phasing pattern for long terminal repeat nucleosomes A and B. Nuclear...

journal_title：Molecular and cellular biology

authors： Archer TK,Cordingley MG,Wolford RG,Hager GL

更新日期：1991-02-01 00:00:00

abstract：:The Saccharomyces cerevisiae CDC25 gene and closely homologous genes in other eukaryotes encode guanine nucleotide exchange factors for Ras proteins. We have determined the minimal region of the budding yeast CDC25 gene capable of activity in vivo. The region required for full biological activity is approximately 450 ...

journal_title：Molecular and cellular biology

authors： Lai CC,Boguski M,Broek D,Powers S

更新日期：1993-03-01 00:00:00

abstract：:Cholera toxin (CT) activates expression of two immediate-early response genes (JE and TIS10) in quiescent BALB/c 3T3 cells. Increases in cyclic AMP (cAMP) levels in response to CT are likely responsible for the induction of TIS10 gene expression, since treatment with 8-Br-cAMP and increasing the intracellular levels o...

journal_title：Molecular and cellular biology

authors： Qureshi SA,Alexandropoulos K,Joseph CK,Spangler R,Foster DA

更新日期：1991-01-01 00:00:00

abstract：:Even though endothelin is recognized as an important vasoregulatory molecule, the roles of endothelin receptors in specific cell types are not yet fully understood. Mice with a null mutation in endothelin A receptor gene (ET(A)) or in the gene of its ligand (endothelin 1) die neonatally due to craniofacial and cardiac...

journal_title：Molecular and cellular biology

authors： Kedzierski RM,Grayburn PA,Kisanuki YY,Williams CS,Hammer RE,Richardson JA,Schneider MD,Yanagisawa M

更新日期：2003-11-01 00:00:00

abstract：:Flap endonuclease 1 (FEN1) has been shown to remove 5' overhanging flap intermediates during base excision repair and to process the 5' ends of Okazaki fragments during lagging-strand DNA replication in vitro. To assess the in vivo role of the mammalian enzyme in repair and replication, we used a gene-targeting approa...

journal_title：Molecular and cellular biology

authors： Larsen E,Gran C,Saether BE,Seeberg E,Klungland A

更新日期：2003-08-01 00:00:00

abstract：:Mos is a germ cell-specific serine/threonine protein kinase that activates mitogen-activated protein kinase (MAPK) through MAPK kinase (MKK). In Xenopus oocytes, Mos synthesis is required for progesterone-induced activation of MAPK and maturation promoting factor. Injection of Mos or active MAPK causes mitotic arrest ...

journal_title：Molecular and cellular biology

authors： Chen M,Cooper JA

更新日期：1995-09-01 00:00:00

abstract：:We investigated various parameters associated with the initial selection of mouse 3T6 cells for resistance to single concentrations of methotrexate and characterized resistant colonies for the presence of additional (amplified) copies of the dihydrofolate reductase gene. Our results indicate that the frequency of occu...

journal_title：Molecular and cellular biology

authors： Brown PC,Tlsty TD,Schimke RT

更新日期：1983-06-01 00:00:00

abstract：:Nuclear factor kappa B (NF-kappa B) is a ubiquitous transcription factor which binds to decameric DNA sequences (kappa B sites) and regulates transcription of multiple genes. The activity of NF-kappa B is regulated by an inhibitor protein, I kappa B, which sequesters NF-kappa B in the cytoplasm. Release of I kappa B a...

journal_title：Molecular and cellular biology

authors： Miyamoto S,Chiao PJ,Verma IM

更新日期：1994-05-01 00:00:00

abstract：:The muscle LIM protein (MLP) and cofilin 2 (CFL2) are important regulators of striated myocyte function. Mutations in the corresponding genes have been directly associated with severe human cardiac and skeletal myopathies, and aberrant expression patterns have often been observed in affected muscles. Herein, we have i...

journal_title：Molecular and cellular biology

authors： Papalouka V,Arvanitis DA,Vafiadaki E,Mavroidis M,Papadodima SA,Spiliopoulou CA,Kremastinos DT,Kranias EG,Sanoudou D

更新日期：2009-11-01 00:00:00

abstract：:A protein with specific affinity for the mRNA cap structure was purified both from the postribosomal supernatant and from the ribosomal high-salt wash of Drosophila melanogaster embryos by m7GTP-Sepharose chromatography. This protein had an apparent molecular mass of 35 kilodaltons (kDa) in sodium dodecyl sulfate-poly...

journal_title：Molecular and cellular biology

authors： Maroto FG,Sierra JM

更新日期：1989-05-01 00:00:00

abstract：:Despite detailed knowledge of the components of the spindle assembly checkpoint, a molecular explanation of how cells die after prolonged spindle checkpoint activation, and thus how microtubule inhibitors and other antimitotic drugs ultimately elicit their lethal effects, has yet to emerge. Mitotically arrested cells ...

journal_title：Molecular and cellular biology

authors： Terrano DT,Upreti M,Chambers TC

更新日期：2010-02-01 00:00:00

abstract：:In the budding yeast Saccharomyces cerevisiae, mutations in the essential gene CDC1 cause defects in Golgi inheritance and actin polarization. However, the biochemical function of Cdc1p is unknown. Previous work showed that cdc1 mutants accumulate intracellular Ca(2+) and display enhanced sensitivity to the extracellu...

journal_title：Molecular and cellular biology

authors： Losev E,Papanikou E,Rossanese OW,Glick BS

更新日期：2008-05-01 00:00:00

abstract：:Nuclear receptors (NRs) regulate diverse physiological processes, including the central nervous system control of energy balance. However, the molecular mechanisms for the central actions of NRs in energy balance remain relatively poorly defined. Here we report a hypothalamic gene network involving two NRs, neuron-der...

journal_title：Molecular and cellular biology

authors： Kim SG,Lee B,Kim DH,Kim J,Lee S,Lee SK,Lee JW

更新日期：2013-10-01 00:00:00

abstract：:We set up an alternative splicing system in vitro in which the relative amounts of two spliced RNAs, one containing and the other lacking a particular exon, were directly proportional to the length of an inverted repeat inserted into the flanking introns. We then used the system to measure the effect of intramolecular...

journal_title：Molecular and cellular biology

authors： Solnick D,Lee SI

更新日期：1987-09-01 00:00:00

abstract：:The intergenic spacer region of the Xenopus laevis ribosomal DNA contains multiple elements which are either 60 or 81 base pairs long. Clusters of these elements have previously been shown to act as position- and distance-independent enhancers on an RNA polymerase I promoter when located in cis. By a combination of de...

journal_title：Molecular and cellular biology

authors： Pikaard CS,Reeder RH

更新日期：1988-10-01 00:00:00

abstract：:Rats and mice have two, equally expressed, nonallelic genes encoding preproinsulin (genes I and II). Cytological hybridization with metaphase chromosomes indicated that both genes reside on rat chromosome I but are approximately 100,000 kilobases apart. In mice the two genes reside on two different chromosomes. DNA se...

journal_title：Molecular and cellular biology

authors： Soares MB,Schon E,Henderson A,Karathanasis SK,Cate R,Zeitlin S,Chirgwin J,Efstratiadis A

更新日期：1985-08-01 00:00:00

abstract：:We have identified an essential cis element in the proximal promoter region of the rat carbamyl phosphate synthetase I (CPSI) gene that is requisite for promoter activity in liver nuclear extracts. Excess synthetic oligonucleotides specifying this region abolished promoter-dependent in vitro transcription. We show tha...

journal_title：Molecular and cellular biology

authors： Howell BW,Lagacé M,Shore GC

更新日期：1989-07-01 00:00:00

abstract：:SR proteins are essential for pre-mRNA splicing in vitro, act early in the splicing pathway, and can influence alternative splice site choice. Here we describe the isolation of both dominant and loss-of-function alleles of B52, the gene for a Drosophila SR protein. The allele B52ED was identified as a dominant second-...

journal_title：Molecular and cellular biology

authors： Peng X,Mount SM

更新日期：1995-11-01 00:00:00

abstract：:Chicken heart mesenchymal cells do not proliferate in medium of physiological composition containing plasma (S. Balk, Proc. Natl. Acad. Sci. USA 77:6606-6610, 1980). To understand the molecular events involved in cell quiescence and in the initiation of cell division under physiological conditions, we examined the dif...

journal_title：Molecular and cellular biology

authors： Bédard PA,Balk SD,Gunther HS,Morisi A,Erikson RL

更新日期：1987-04-01 00:00:00

abstract：:The mechanism by which the maize autonomous Ac transposable element gives rise to nonautonomous Ds elements is largely unknown. Sequence analysis of native maize Ds elements indicates a complex chimeric structure formed through deletions of Ac sequences with or without insertions of Ac-unrelated sequence blocks. These...

journal_title：Molecular and cellular biology

authors： Rubin E,Levy AA

更新日期：1997-11-01 00:00:00

abstract：:The heterochromatic domains of Drosophila melanogaster (pericentric heterochromatin, telomeres, and the fourth chromosome) are characterized by histone hypoacetylation, high levels of histone H3 methylated on lysine 9 (H3-mK9), and association with heterochromatin protein 1 (HP1). While the specific interaction of HP1...

journal_title：Molecular and cellular biology

authors： Sun FL,Haynes K,Simpson CL,Lee SD,Collins L,Wuller J,Eissenberg JC,Elgin SC

更新日期：2004-09-01 00:00:00

abstract：:Intestinal alkaline phosphatase (IAP) is the most ancestral of the tissue-specific members of the AP gene family. Several studies have suggested an absorptive function for IAP, but in vivo data to this effect have been lacking. We inactivated the mouse IAP gene in embryo-derived stem cells and generated mice homozygou...

journal_title：Molecular and cellular biology

authors： Narisawa S,Huang L,Iwasaki A,Hasegawa H,Alpers DH,Millán JL

更新日期：2003-11-01 00:00:00

abstract：:Calcineurin, or PP2B, plays a critical role in mediating Ca2+-dependent signaling in many cell types. In yeast cells, this highly conserved protein phosphatase regulates aspects of ion homeostasis and cell wall synthesis. We show that calcineurin mutants are sensitive to high concentrations of Mn2+ and identify two ge...

journal_title：Molecular and cellular biology

authors： Pozos TC,Sekler I,Cyert MS

更新日期：1996-07-01 00:00:00

abstract：:Despite the identification of PBC proteins as cofactors that provide DNA affinity and binding specificity for the HOX homeodomain proteins, HOX proteins do not demonstrate robust activity in transient-transcription assays and few authentic downstream targets have been identified for these putative transcription factor...

journal_title：Molecular and cellular biology

authors： Shen WF,Krishnan K,Lawrence HJ,Largman C

更新日期：2001-11-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (RB) is a potent inhibitor of cell proliferation. RB is expressed throughout the cell cycle, but its antiproliferative activity is neutralized by phosphorylation during the G(1)/S transition. RB plays an essential role in the G(1) arrest induced by a variety of growth inhibi...

journal_title：Molecular and cellular biology

authors： Knudsen KE,Booth D,Naderi S,Sever-Chroneos Z,Fribourg AF,Hunton IC,Feramisco JR,Wang JY,Knudsen ES

更新日期：2000-10-01 00:00:00

abstract：:RBM4 promotes differentiation of neuronal progenitor cells and neurite outgrowth of cultured neurons via its role in splicing regulation. In this study, we further explored the role of RBM4 in neuronal differentiation. During neuronal differentiation, energy production shifts from glycolysis to oxidative phosphorylati...

journal_title：Molecular and cellular biology

authors： Su CH,Hung KY,Hung SC,Tarn WY

更新日期：2017-01-19 00:00:00

abstract：:Cellular senescence is a tumor-suppressive process characterized by an irreversible cell cycle exit, a unique morphology, and expression of senescence-associated beta-galactosidase (SA-beta-Gal). We report here a role for CDK5 in induction of senescent cytoskeletal changes. CDK5 activation is upregulated in senescing ...

journal_title：Molecular and cellular biology

authors： Alexander K,Yang HS,Hinds PW

更新日期：2004-04-01 00:00:00