Proliferation failure and gamma radiation sensitivity of Fen1 null mutant mice at the blastocyst stage.

abstract：:A new family of highly conserved genes, designated rho, has recently been isolated and characterized (P. Madaule and R. Axel, Cell 41:31-40, 1985). These genes have been found in Saccharomyces cerevisiae, Drosophila melanogaster, rats, and humans, and their 21,000-dalton products are highly homologous. The rho p21 pro...

journal_title：Molecular and cellular biology

authors： Anderson PS,Lacal JC

更新日期：1987-10-01 00:00:00

abstract：:DET1 (de-etiolated 1) is an essential negative regulator of plant light responses, and it is a component of the Arabidopsis thaliana CDD complex containing DDB1 and COP10 ubiquitin E2 variant. Human DET1 has recently been isolated as one of the DDB1- and Cul4A-associated factors, along with an array of WD40-containing...

journal_title：Molecular and cellular biology

authors： Pick E,Lau OS,Tsuge T,Menon S,Tong Y,Dohmae N,Plafker SM,Deng XW,Wei N

更新日期：2007-07-01 00:00:00

abstract：:It is well established that steroid receptor function requires interaction with coactivators. However, the mechanisms through which steroid receptors elicit precise assembly of coactivator complexes and the way the steroid activation signal is transduced remain elusive. Using a T47D cell line stably integrated with a ...

journal_title：Molecular and cellular biology

authors： Li X,Wong J,Tsai SY,Tsai MJ,O'Malley BW

更新日期：2003-06-01 00:00:00

abstract：:Low-molecular-weight RNA exhibiting transforming potential was identified in chemically induced lymphoma cells by the transformation of mink lung cells after transfection. The RNA was sequenced by the direct chemical method and was shown to be a small nuclear RNA, U5. The transforming potential of the RNA was further ...

journal_title：Molecular and cellular biology

authors： Hamada K,Kumazaki T,Mizuno K,Yokoro K

更新日期：1989-10-01 00:00:00

abstract：:Dominant and recessive mutations at the SSN20 locus were previously isolated as extragenic suppressors of mutations in three genes (SNF2, SNF5, and SNF6) that are required in trans to derepress invertase expression. All ssn20 alleles cause recessive, temperature-sensitive lethality. In this study we cloned the SSN20 g...

journal_title：Molecular and cellular biology

authors： Neigeborn L,Celenza JL,Carlson M

更新日期：1987-02-01 00:00:00

abstract：:We examined the effects of 18 h of incubation of Chinese hamster ovary (CHO K1) cells with cycloheximide, hydroxyurea, and aphidicolin. Treatment of cells with cycloheximide alone at a concentration adequate to inhibit DNA synthesis to less than 10% of control was significantly less cytotoxic and clastogenic than trea...

journal_title：Molecular and cellular biology

authors： Sherwood SW,Schumacher RI,Schimke RT

更新日期：1988-07-01 00:00:00

abstract：:The interaction of enhancers with different promoters was studied by measuring the influence of two enhancers (from simian virus 40 and from Harvey sarcoma virus) on the activity of expression vectors that are identical except for their promoter region. The promoters examined were from the simian virus 40 early region...

journal_title：Molecular and cellular biology

authors： Berg PE,Popovic Z,Anderson WF

更新日期：1984-08-01 00:00:00

abstract：:Previous studies have shown that exposure of cells to high levels of replicational stress leads to permanent proliferation arrest that does not require p53. We have examined cellular responses to therapeutically relevant low levels of replicational stress that allow limited proliferation. Chronic exposure to low conce...

journal_title：Molecular and cellular biology

authors： Marusyk A,Wheeler LJ,Mathews CK,DeGregori J

更新日期：2007-08-01 00:00:00

abstract：:Protein ubiquitination plays a key role in the regulation of a variety of DNA repair mechanisms. Protein ubiquitination is controlled by the coordinate activity of ubiquitin ligases and deubiquitinating enzymes (DUBs). The deubiquitinating enzyme USP1 regulates DNA repair and the Fanconi anemia pathway through its ass...

journal_title：Molecular and cellular biology

authors： Murai J,Yang K,Dejsuphong D,Hirota K,Takeda S,D'Andrea AD

更新日期：2011-06-01 00:00:00

abstract：:Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae. Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which p...

journal_title：Molecular and cellular biology

authors： Knight SA,Tamai KT,Kosman DJ,Thiele DJ

更新日期：1994-12-01 00:00:00

abstract：:Nuclear receptors (NRs) regulate diverse physiological processes, including the central nervous system control of energy balance. However, the molecular mechanisms for the central actions of NRs in energy balance remain relatively poorly defined. Here we report a hypothalamic gene network involving two NRs, neuron-der...

journal_title：Molecular and cellular biology

authors： Kim SG,Lee B,Kim DH,Kim J,Lee S,Lee SK,Lee JW

更新日期：2013-10-01 00:00:00

abstract：:Receptor tyrosine kinases (RTKs) play distinct roles in multiple biological systems. Many RTKs transmit similar signals, raising questions about how specificity is achieved. One potential mechanism for RTK specificity is control of the magnitude and kinetics of activation of downstream pathways. We have found that the...

journal_title：Molecular and cellular biology

authors： Zhang SQ,Tsiaras WG,Araki T,Wen G,Minichiello L,Klein R,Neel BG

更新日期：2002-06-01 00:00:00

abstract：:A truncated H-2Ld gene was constructed by deleting the transmembrane and cytoplasmic exons. The truncated H-2Ld gene was introduced into mouse L cells using the thymidine kinase gene as a selectable marker. Transformants were isolated and screened for the presence of truncated H-2Ld antigen. The truncated H-2Ld gene p...

journal_title：Molecular and cellular biology

authors： Murre C,Parker KC,Reiss CS,Driller JP,Wiley DC,Burakoff SJ,Seidman JG

更新日期：1986-04-01 00:00:00

abstract：:In order to determine whether partial methylation of the herpes simplex virus (HSV) tk gene prevents tk gene expression, the HSV tk gene was cloned as single-stranded DNA. By in vitro second-strand DNA synthesis, specific HSV tk gene segments were methylated, and the hemimethylated DNA molecules were microinjected int...

journal_title：Molecular and cellular biology

authors： Graessmann A,Sandberg G,Guhl E,Graessmann M

更新日期：1994-03-01 00:00:00

abstract：:To investigate the mechanism of action of volatile anesthetics, we are studying mutants of the yeast Saccharomyces cerevisiae that have altered sensitivity to isoflurane, a widely used clinical anesthetic. Several lines of evidence from these studies implicate a role for ubiquitin metabolism in cellular response to vo...

journal_title：Molecular and cellular biology

authors： Wolfe D,Reiner T,Keeley JL,Pizzini M,Keil RL

更新日期：1999-12-01 00:00:00

abstract：:Tristetraprolin (TTP) is a tandem CCCH zinc finger protein that was identified through its rapid induction by mitogens in fibroblasts. Studies of TTP-deficient mice and cells derived from them showed that TTP could bind to certain AU-rich elements in mRNAs, leading to increases in the rates of mRNA deadenylation and d...

journal_title：Molecular and cellular biology

authors： Lai WS,Parker JS,Grissom SF,Stumpo DJ,Blackshear PJ

更新日期：2006-12-01 00:00:00

abstract：:Growth factors and cyclic AMP (cAMP) are known to activate distinct intracellular signaling pathways. Fibroblast growth factor (FGF) activates ras-dependent kinase cascades, resulting in the activation of MAP kinases, whereas cAMP activates protein kinase A. In this study, we report that growth factors and cAMP act sy...

journal_title：Molecular and cellular biology

authors： Tan Y,Low KG,Boccia C,Grossman J,Comb MJ

更新日期：1994-11-01 00:00:00

abstract：:An unusual glucocorticoid-responsive element (called GRE A) was found to mediate the induction of the cytosolic aspartate aminotransferase gene by glucocorticoids and was bound by the glucocorticoid receptor in a DNase I footprinting assay. GRE A consists of two overlapping GREs, each comprising a conserved half-site ...

journal_title：Molecular and cellular biology

authors： Garlatti M,Daheshia M,Slater E,Bouguet J,Hanoune J,Beato M,Barouki R

更新日期：1994-12-01 00:00:00

abstract：:Activation of oncogenes or inhibition of WEE1 kinase deregulates cyclin-dependent kinase (CDK) activity and leads to replication stress; however, the underlying mechanism is not understood. We now show that elevation of CDK activity by inhibition of WEE1 kinase rapidly increases initiation of replication. This leads t...

journal_title：Molecular and cellular biology

authors： Beck H,Nähse-Kumpf V,Larsen MS,O'Hanlon KA,Patzke S,Holmberg C,Mejlvang J,Groth A,Nielsen O,Syljuåsen RG,Sørensen CS

更新日期：2012-10-01 00:00:00

abstract：:The mechanisms underlying the circadian control of gene expression in peripheral tissues and influencing many biological pathways are poorly defined. Factor VII (FVII), the protease triggering blood coagulation, represents a valuable model to address this issue in liver since its plasma levels oscillate in a circadian...

journal_title：Molecular and cellular biology

authors： Bertolucci C,Cavallari N,Colognesi I,Aguzzi J,Chen Z,Caruso P,Foá A,Tosini G,Bernardi F,Pinotti M

更新日期：2008-05-01 00:00:00

abstract：:Unlike Saccharomyces cerevisiae RNA polymerase III, human RNA polymerase III has not been entirely characterized. Orthologues of the yeast RNA polymerase III subunits C128 and C37 remain unidentified, and for many of the other subunits, the available information is limited to database sequences with various degrees of...

journal_title：Molecular and cellular biology

authors： Hu P,Wu S,Sun Y,Yuan CC,Kobayashi R,Myers MP,Hernandez N

更新日期：2002-11-01 00:00:00

abstract：:Mammalian B-cell lymphoid malignancies frequently display aberrant translocations involving the c-myc proto-oncogene and one of the immunoglobulin loci. We have observed and characterized such a translocation in the immunoglobulin E-producing rat immunocytoma IR162 by using recombinant DNA technology. We show here for...

journal_title：Molecular and cellular biology

authors： Tian SS,Faust C

更新日期：1987-07-01 00:00:00

abstract：:The eukaryotic SNM1 gene family has been implicated in a number of cellular pathways, including repair of DNA interstrand cross-links, involvement in VDJ recombination, repair of DNA double-strand breaks, and participation in cell cycle checkpoint pathways. In particular, mammalian SNM1 has been shown to be required i...

journal_title：Molecular and cellular biology

authors： Ahkter S,Richie CT,Zhang N,Behringer RR,Zhu C,Legerski RJ

更新日期：2005-11-01 00:00:00

abstract：:One of the central questions in eukaryotic transcription is how activators can transmit their signal to stimulate gene expression in the context of chromatin. The multisubunit SAGA coactivator complex has both histone acetyltransferase and deubiquitination activities and remodels chromatin to allow transcription. Whet...

journal_title：Molecular and cellular biology

authors： Nagy Z,Riss A,Romier C,le Guezennec X,Dongre AR,Orpinell M,Han J,Stunnenberg H,Tora L

更新日期：2009-03-01 00:00:00

abstract：:Bone metastasis is the hallmark of progressive and castration-resistant prostate cancers. MicroRNA 1 (miR-1) levels are decreased in clinical samples of primary prostate cancer and further reduced in metastases. SRC has been implicated as a critical factor in bone metastasis, and here we show that SRC is a direct targ...

journal_title：Molecular and cellular biology

authors： Liu YN,Yin J,Barrett B,Sheppard-Tillman H,Li D,Casey OM,Fang L,Hynes PG,Ameri AH,Kelly K

更新日期：2015-06-01 00:00:00

abstract：:Retinoblastoma (RB) tumors develop when both alleles of a gene (RB1) are mutated and unable to function normally. Recently, Friend et al. [S. H. Friend, R. Bernards, S. Rogelj, R. A. Weinberg, J. M. Rapaport, D. M. Albert, and T. P. Dryja, Nature (London) 32:643-646, 1986] reported the cloning of a gene, 4.7R, with so...

journal_title：Molecular and cellular biology

authors： Goddard AD,Balakier H,Canton M,Dunn J,Squire J,Reyes E,Becker A,Phillips RA,Gallie BL

更新日期：1988-05-01 00:00:00

abstract：:Cyclic AMP-responsive element-binding protein 3-like 3, hepatocyte specific (CREBH), is a hepatic transcription factor that functions as a key regulator of energy homeostasis. Here, we defined a regulatory CREBH posttranslational modification process, namely, lysine-specific acetylation, and its functional involvement...

journal_title：Molecular and cellular biology

authors： Kim H,Mendez R,Chen X,Fang D,Zhang K

更新日期：2015-12-01 00:00:00

abstract：:This paper presents a general means of eliminating the function of a single protein without relying on genetic alterations in its structure or level of synthesis. The strategy is based on the inducible cellular expression of neutralizing antibody to inactivate the protein selectively. The feasibility of this approach ...

journal_title：Molecular and cellular biology

authors： Carlson JR

更新日期：1988-06-01 00:00:00

abstract：:Two alleles of the Drosophila melanogaster Rfc4 (DmRfc4) gene, which encodes subunit 4 of the replication factor C (RFC) complex, cause striking defects in mitotic chromosome cohesion and condensation. These mutations produce larval phenotypes consistent with a role in DNA replication but also result in mitotic chromo...

journal_title：Molecular and cellular biology

authors： Krause SA,Loupart ML,Vass S,Schoenfelder S,Harrison S,Heck MM

更新日期：2001-08-01 00:00:00

abstract：:In vitro experiments with cell extracts prepared from a mouse mammary epithelial cell line demonstrated that a cis-acting glucocorticoid response element (GRE) of the mouse mammary tumor virus represses transcription from its homologous promoter. Competition transcription experiments, in which a molar excess of a rest...

journal_title：Molecular and cellular biology

authors： Langer SJ,Ostrowski MC

更新日期：1988-09-01 00:00:00