Progesterone and glucocorticoid receptors recruit distinct coactivator complexes and promote distinct patterns of local chromatin modification.

abstract：:The multisubunit yeast transcription factor IIIC (TFIIIC) is a multifunctional protein required for promoter recognition, transcription factor IIIB recruitment, and chromatin antirepression. We report the isolation and characterization of TFC7, an essential gene encoding the 55-kDa polypeptide, tau55, present in affin...

journal_title：Molecular and cellular biology

authors： Manaud N,Arrebola R,Buffin-Meyer B,Lefebvre O,Voss H,Riva M,Conesa C,Sentenac A

更新日期：1998-06-01 00:00:00

abstract：:Nuclear pore complexes (NPCs) play an essential role in RNA export. Nucleoporins required for mRNA export in Saccharomyces cerevisiae are found in the Nup84p and Nup82p subcomplexes of the NPC. The Nup82p subcomplex contains Nup82p, Rat7p/Nup159p, Nsp1p, Gle1p/Rss1p, and Rip1p/Nup42p and is found only on the cytoplasm...

journal_title：Molecular and cellular biology

authors： Rollenhagen C,Hodge CA,Cole CN

更新日期：2004-06-01 00:00:00

abstract：:Previous studies indicated that repression by eve involves cooperative DNA binding and leads to the formation of a DNA loop which encompasses the DNA sequences normally bound by the RNA polymerase II general transcription factors. To test the general principle of whether bending of a basal promoter sequence can contri...

journal_title：Molecular and cellular biology

authors： TenHarmsel A,Biggin MD

更新日期：1995-10-01 00:00:00

abstract：:In Saccharomyces cerevisiae, COX5a and COX5b encode two distinct forms of cytochrome c oxidase subunit V, Va and Vb, respectively. To determine the relative contribution of COX5a and COX5b to cytochrome c oxidase function, we have disrupted each gene. Cytochrome c oxidase activity levels and respiration rates of strai...

journal_title：Molecular and cellular biology

authors： Trueblood CE,Poyton RO

更新日期：1987-10-01 00:00:00

abstract：:The tetraspanins are a family of integral membrane proteins with four transmembrane domains. These molecules form multimolecular networks on the surfaces of many different cell types. Gene-targeting studies have revealed a role for tetraspanins in B- and T-lymphocyte function. We have isolated and deleted a novel tetr...

journal_title：Molecular and cellular biology

authors： Tarrant JM,Groom J,Metcalf D,Li R,Borobokas B,Wright MD,Tarlinton D,Robb L

更新日期：2002-07-01 00:00:00

abstract：:Thyroid hormone receptors are encoded by the TRalpha (NR1A1) and TRbeta (NR1A2) loci. These genes are transcribed into multiple variants whose functions are unclear. Analysis by gene inactivation in mice has provided new insights into the functional complexity of these products. Different strategies designed to modify...

journal_title：Molecular and cellular biology

authors： Gauthier K,Plateroti M,Harvey CB,Williams GR,Weiss RE,Refetoff S,Willott JF,Sundin V,Roux JP,Malaval L,Hara M,Samarut J,Chassande O

更新日期：2001-07-01 00:00:00

abstract：:Multiple organ systems require epithelial barriers for normal function, and barrier loss is a hallmark of diseases ranging from inflammation to epithelial cancers. However, the molecular processes regulating epithelial barrier maturation are not fully elucidated. After contact, epithelial cells undergo size-reductive ...

journal_title：Molecular and cellular biology

authors： Bolinger MT,Ramshekar A,Waldschmidt HV,Larsen SD,Bewley MC,Flanagan JM,Antonetti DA

更新日期：2016-07-14 00:00:00

abstract：:Kinase-negative mutants of S49 mouse lymphoma cells, which lack detectable catalytic (C) subunit of cyclic AMP-dependent protein kinase, nevertheless contain cytoplasmic mRNAs for the two major forms of C subunit, C alpha and C beta. Investigation of the metabolism of C subunits in wild-type and mutant cells was under...

journal_title：Molecular and cellular biology

authors： Steinberg RA

更新日期：1991-02-01 00:00:00

abstract：:Transcriptional activation of eukaryotic genes depends on the precise and ordered recruitment of activators, chromatin modifiers/remodelers, coactivators, and general transcription factors to the promoters of target genes. Using the human matrix metalloproteinase 9 (MMP-9) gene as a model system, we investigated the s...

journal_title：Molecular and cellular biology

authors： Ma Z,Shah RC,Chang MJ,Benveniste EN

更新日期：2004-06-01 00:00:00

abstract：:The expression of c-myc proto-oncogene, a key regulator of cell proliferation and apoptosis, is controlled at different transcriptional and posttranscriptional levels. In particular, the c-myc mRNA contains an internal ribosome entry site (IRES) able to promote translation initiation independently from the classical c...

journal_title：Molecular and cellular biology

authors： Créancier L,Mercier P,Prats AC,Morello D

更新日期：2001-03-01 00:00:00

abstract：:Ewing's family tumor (EFT) is a rare pediatric tumor of unclear origin that occurs in bone and soft tissue. Specific chromosomal translocations found in EFT cause EWS to fuse to a subset of ets transcription factor genes (ETS), generating chimeric EWS/ETS proteins. These proteins are believed to play a crucial role in...

journal_title：Molecular and cellular biology

authors： Miyagawa Y,Okita H,Nakaijima H,Horiuchi Y,Sato B,Taguchi T,Toyoda M,Katagiri YU,Fujimoto J,Hata J,Umezawa A,Kiyokawa N

更新日期：2008-04-01 00:00:00

abstract：:The binding of the U1 small nuclear ribonucleoprotein (snRNP)-specific proteins C, A, and 70K to U1 small nuclear RNA (snRNA) was analyzed. Assembly of U1 snRNAs from bean and soybean and a set of mutant Xenopus U1 snRNAs into U1 snRNPs in Xenopus egg extracts was studied. The ability to bind proteins was analyzed by ...

journal_title：Molecular and cellular biology

authors： Hamm J,van Santen VL,Spritz RA,Mattaj IW

更新日期：1988-11-01 00:00:00

abstract：:We examined the mechanisms that control the downregulation of the c-myc mRNA during differentiation of HL60 cells. On treatment with dimethyl sulfoxide, HL60 cells downmodulated their steady-state c-myc message levels, ceased to proliferate, and underwent terminal differentiation. In nuclear run-on assays in which dis...

journal_title：Molecular and cellular biology

authors： Siebenlist U,Bressler P,Kelly K

更新日期：1988-02-01 00:00:00

abstract：:The numerous members of the steroid/nuclear hormone receptor superfamily act as direct transducers of circulating signals, such as steroids, thyroid hormone, and vitamin or lipid metabolites, and modulate the transcription of specific target genes, primarily as dimeric complexes. The receptors for 9-cis retinoic acid ...

journal_title：Molecular and cellular biology

authors： Lemon BD,Fondell JD,Freedman LP

更新日期：1997-04-01 00:00:00

abstract：:Posttranslational modifications (PTMs) can alter many fundamental properties of a protein. One or combinations of them have been known to regulate the dynamics of many cellular pathways and consequently regulate all vital processes. Understandably, pathogens have evolved sophisticated strategies to subvert these mecha...

journal_title：Molecular and cellular biology

authors： Verma S,Mohapatra G,Ahmad SM,Rana S,Jain S,Khalsa JK,Srikanth CV

更新日期：2015-09-01 00:00:00

abstract：:The nucleotide sequence of 5'-noncoding and N-terminal coding regions of two coordinately regulated, repressible acid phosphatase genes from Saccharomyces cerevisiae were determined. These unlinked genes encode different, but structurally related polypeptides of molecular weights 60,000 and 56,000. The DNA sequences o...

journal_title：Molecular and cellular biology

authors： Thill GP,Kramer RA,Turner KJ,Bostian KA

更新日期：1983-04-01 00:00:00

abstract：:Extracellular signal-regulated kinase (ERK) facilitates cell cycle progression in most mammalian cells, but in certain cell types prolonged signaling through this pathway promotes differentiation and lineage-specific gene expression through mechanisms that are poorly understood. Here, we characterize the transcription...

journal_title：Molecular and cellular biology

authors： Sevinsky JR,Whalen AM,Ahn NG

更新日期：2004-05-01 00:00:00

abstract：:Bona fide apoptosis in rat and human leukemia cells, rat thymocytes, and bovine endothelial cells was accompanied by limited and specific cleavage of polysome-associated and monosome-associated 28S rRNA, with 18S rRNA being spared. Specific 28S rRNA cleavage was observed in all instances of apoptotic death accompanied...

journal_title：Molecular and cellular biology

authors： Houge G,Robaye B,Eikhom TS,Golstein J,Mellgren G,Gjertsen BT,Lanotte M,Døskeland SO

更新日期：1995-04-01 00:00:00

abstract：:In Aspergillus nidulans, a fungus that possesses negligible, if any, levels of methylation in its genome, low concentrations of 5-azacytidine (5-AC) convert a high percentage of the cell population to fluffy phenotypic variants through a heritable modification of a single nuclear gene (M. Tamame, F. Antequera, J. R. V...

journal_title：Molecular and cellular biology

authors： Tamame M,Antequera F,Santos E

更新日期：1988-08-01 00:00:00

abstract：:We have investigated the site of surface expression of the neuraminidase (NA) glycoprotein of influenza A virus, which, in contrast to the hemagglutinin, is bound to membranes by hydrophobic residues near the NH2-terminus. Madin-Darby canine kidney or primary African green monkey kidney cells infected with influenza A...

journal_title：Molecular and cellular biology

authors： Jones LV,Compans RW,Davis AR,Bos TJ,Nayak DP

更新日期：1985-09-01 00:00:00

abstract：:Tax corresponds to a 40-kDa transforming protein from the pathogenic retrovirus human T-cell leukemia virus type 1 (HTLV-1) that activates nuclear expression of the NF-kappaB/Rel family of transcription factors by an unknown mechanism. Tax expression promotes N-terminal phosphorylation and degradation of IkappaB alpha...

journal_title：Molecular and cellular biology

authors： Geleziunas R,Ferrell S,Lin X,Mu Y,Cunningham ET Jr,Grant M,Connelly MA,Hambor JE,Marcu KB,Greene WC

更新日期：1998-09-01 00:00:00

abstract：:The estrogen receptor (ER) modulates transcription by forming complexes with other proteins and then binding to the estrogen response element (ERE). We have identified a novel interaction of this receptor with the POU transcription factors Brn-3a and Brn-3b which was independent of ligand binding. By pull-down assays ...

journal_title：Molecular and cellular biology

authors： Budhram-Mahadeo V,Parker M,Latchman DS

更新日期：1998-02-01 00:00:00

abstract：:Obesity and type 2 diabetes are characterized by insulin resistance. Mice lacking the protein-tyrosine phosphatase PTP1B in all tissues are hypersensitive to insulin but also have diminished fat stores. Because adiposity affects insulin sensitivity, the extent to which PTP1B directly regulates glucose homeostasis has ...

journal_title：Molecular and cellular biology

authors： Delibegovic M,Bence KK,Mody N,Hong EG,Ko HJ,Kim JK,Kahn BB,Neel BG

更新日期：2007-11-01 00:00:00

abstract：:U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviat...

journal_title：Molecular and cellular biology

authors： Xu GL,Wieland B,Bindereif A

更新日期：1994-07-01 00:00:00

abstract：:The transcriptional activator ADR1 from Saccharomyces cerevisiae is a postulated DNA-binding protein that controls the expression of the glucose-repressible alcohol dehydrogenase (ADH2). Carboxy-terminal deletions of the ADR1 protein (1,323 amino acids in length) were used to localize its functional regions. The trans...

journal_title：Molecular and cellular biology

authors： Bemis LT,Denis CL

更新日期：1988-05-01 00:00:00

abstract：:Cytochrome P450 oxidoreductase (POR) is the obligate electron donor for all microsomal cytochrome P450 enzymes, which catalyze the metabolism of a wide spectrum of xenobiotic and endobiotic compounds. Point mutations in POR have been found recently in patients with Antley-Bixler-like syndrome, which includes limb skel...

journal_title：Molecular and cellular biology

authors： Schmidt K,Hughes C,Chudek JA,Goodyear SR,Aspden RM,Talbot R,Gundersen TE,Blomhoff R,Henderson C,Wolf CR,Tickle C

更新日期：2009-05-01 00:00:00

abstract：:Approximately half of the introns in Drosophila melanogaster are too small to function in a vertebrate and often lack the pyrimidine tract associated with vertebrate 3' splice sites. Here, we report the splicing and spliceosome assembly properties of two such introns: one with a pyrimidine-poor 3' splice site and one ...

journal_title：Molecular and cellular biology

authors： Talerico M,Berget SM

更新日期：1994-05-01 00:00:00

abstract：:The human glycoprotein IIB (GPIIB) gene is expressed only in megakaryocytes, and its promoter displays cell type specificity. We show that this specificity involved two cis-acting sequences. The first one, located at -55, contains a GATA binding site. Point mutations that abolish protein binding on this site decrease ...

journal_title：Molecular and cellular biology

authors： Lemarchandel V,Ghysdael J,Mignotte V,Rahuel C,Roméo PH

更新日期：1993-01-01 00:00:00

abstract：:Activation of the Evi-1 zinc finger gene is a common event associated with transformation of murine myeloid leukemias. To characterize the gene product, we developed antisera against various protein domains. These antisera primarily detected a 145-kilodalton nuclear protein that bound double-stranded DNA. Binding was ...

journal_title：Molecular and cellular biology

authors： Matsugi T,Morishita K,Ihle JN

更新日期：1990-03-01 00:00:00

abstract：:We have identified multiple distinct splicing enhancer elements within protein-coding sequences of the constitutively spliced human beta-globin pre-mRNA. Each of these highly conserved sequences is sufficient to activate the splicing of a heterologous enhancer-dependent pre-mRNA. One of these enhancers is activated by...

journal_title：Molecular and cellular biology

authors： Schaal TD,Maniatis T

更新日期：1999-01-01 00:00:00