Fibroblast growth factor and cyclic AMP (cAMP) synergistically activate gene expression at a cAMP response element.

abstract：:Using a subtractive cloning scheme on cDNA prepared from primary pro-B and pre-B cells, we identified several genes whose products regulate apoptosis. We further characterized one of these genes, encoding protein kinase Ceta (PKCeta). PKCeta transcripts were readily detected in pro-B cells but were absent in pre-B cel...

journal_title：Molecular and cellular biology

authors： Morrow TA,Muljo SA,Zhang J,Hardwick JM,Schlissel MS

更新日期：1999-08-01 00:00:00

abstract：:We have isolated and subcloned three separate segments of human DNA which share strong sequence homology with a previously sequenced gene encoding a type I keratin, K14 (50 kilodaltons). Restriction endonuclease mapping has demonstrated that these three genes are tightly linked chromosomally, whereas the K14 gene appe...

journal_title：Molecular and cellular biology

authors： RayChaudhury A,Marchuk D,Lindhurst M,Fuchs E

更新日期：1986-02-01 00:00:00

abstract：:Bone morphogenetic protein 7 (BMP7) stimulates renal branching morphogenesis via p38 mitogen-activated protein kinase (p38(MAPK)) and activating transcription factor 2 (ATF-2) (M. C. Hu, D. Wasserman, S. Hartwig, and N. D. Rosenblum, J. Biol. Chem. 279:12051-12059, 2004). Here, we demonstrate a novel role for integrin...

journal_title：Molecular and cellular biology

authors： Leung-Hagesteijn C,Hu MC,Mahendra AS,Hartwig S,Klamut HJ,Rosenblum ND,Hannigan GE

更新日期：2005-05-01 00:00:00

abstract：:In the mouse mammary tumor virus promoter, a tandem of octamer motifs, recognized by ubiquitous and tissue-restricted Oct transcription factors, is located upstream of the TATA box and next to a binding site for the transcription factor nuclear factor I (NF-I). Their function was investigated with mutant long terminal...

journal_title：Molecular and cellular biology

authors： Buetti E

更新日期：1994-02-01 00:00:00

abstract：:Chicken embryo fibroblast cells overexpressing activated mutant forms of human pp60c-src, but not those overexpressing normal human pp60c-src, exhibited high levels of type I phosphatidylinositol (PI) kinase activity associated with pp60c-src. Levels of PI kinase activity were positively correlated with src tyrosine p...

journal_title：Molecular and cellular biology

authors： Chan TO,Tanaka A,Bjorge JD,Fujita DJ

更新日期：1990-06-01 00:00:00

abstract：:Chromosomal rearrangements associated with one Ty1 element in the iso-1-cytochrome c (CYC1) region of Saccharomyces cerevisiae yeast cells were examined. Most of the rearrangements were deletions of the three linked genes, CYC1, OSM1, and RAD7, and resulted from recombination involving the single Ty1 element and a sol...

journal_title：Molecular and cellular biology

authors： Downs KM,Brennan G,Liebman SW

更新日期：1985-12-01 00:00:00

abstract：:The Ets family transcription factor PU.1 is crucial for the regulation of hematopoietic development. Pu.1 is activated in hematopoietic stem cells and is expressed in mast cells, B cells, granulocytes, and macrophages but is switched off in T cells. Many of the transcription factors regulating Pu.1 have been identifie...

journal_title：Molecular and cellular biology

authors： Hoogenkamp M,Krysinska H,Ingram R,Huang G,Barlow R,Clarke D,Ebralidze A,Zhang P,Tagoh H,Cockerill PN,Tenen DG,Bonifer C

更新日期：2007-11-01 00:00:00

abstract：:Transcriptional activation of eukaryotic genes depends on the precise and ordered recruitment of activators, chromatin modifiers/remodelers, coactivators, and general transcription factors to the promoters of target genes. Using the human matrix metalloproteinase 9 (MMP-9) gene as a model system, we investigated the s...

journal_title：Molecular and cellular biology

authors： Ma Z,Shah RC,Chang MJ,Benveniste EN

更新日期：2004-06-01 00:00:00

abstract：:TRPV5 and TRPV6 are the most Ca2+-selective members of the transient receptor potential (TRP) family of cation channels and play a pivotal role in the maintenance of Ca2+ balance in the body. However, little is known about the mechanisms controlling the plasma membrane abundance of these channels to regulate epithelia...

journal_title：Molecular and cellular biology

authors： van de Graaf SF,Chang Q,Mensenkamp AR,Hoenderop JG,Bindels RJ

更新日期：2006-01-01 00:00:00

abstract：:In vitro experiments with cell extracts prepared from a mouse mammary epithelial cell line demonstrated that a cis-acting glucocorticoid response element (GRE) of the mouse mammary tumor virus represses transcription from its homologous promoter. Competition transcription experiments, in which a molar excess of a rest...

journal_title：Molecular and cellular biology

authors： Langer SJ,Ostrowski MC

更新日期：1988-09-01 00:00:00

abstract：:Forkhead (Fkh) transcription factors influence cell death, proliferation, and differentiation and the cell cycle. In Saccharomyces cerevisiae, Fkh2 both activates and represses transcription of CLB2, encoding a B-type cyclin. CLB2 is expressed during G(2)/M phase and repressed during G(1). Fkh2 recruits the coactivato...

journal_title：Molecular and cellular biology

authors： Sherriff JA,Kent NA,Mellor J

更新日期：2007-04-01 00:00:00

abstract：:mof6-1 was originally isolated as a recessive mutation in Saccharomyces cerevisiae which promoted increased efficiencies of programmed -1 ribosomal frameshifting and rendered cells unable to maintain the killer virus. Here, we demonstrate that mof6-1 is a unique allele of the histone deacetylase RPD3, that the deacety...

journal_title：Molecular and cellular biology

authors： Meskauskas A,Baxter JL,Carr EA,Yasenchak J,Gallagher JE,Baserga SJ,Dinman JD

更新日期：2003-03-01 00:00:00

abstract：:Numb proteins are evolutionarily conserved signaling molecules that make the daughter cells different after asymmetric divisions by segregating to only one daughter. They contain distinct binding motifs for alpha-adaptin (alpha-Ada) and proteins with Eps15 homology (EH) domains, which regulate endocytosis, and for E3 ...

journal_title：Molecular and cellular biology

authors： Tang H,Rompani SB,Atkins JB,Zhou Y,Osterwalder T,Zhong W

更新日期：2005-04-01 00:00:00

abstract：:The genes of the trithorax group (trxG) in Drosophila melanogaster are required to maintain the pattern of homeotic gene expression that is established early in embryogenesis by the transient expression of the segmentation genes. The precise role of each of the diverse trxG members and the functional relationships amo...

journal_title：Molecular and cellular biology

authors： Crosby MA,Miller C,Alon T,Watson KL,Verrijzer CP,Goldman-Levi R,Zak NB

更新日期：1999-02-01 00:00:00

abstract：:Adiponectin is a secretory protein abundantly secreted from adipocytes. It assembles into a number of different higher-order complexes. Adipocytes maintain tight control over circulating plasma levels, suggesting the existence of a complex, highly regulated biosynthetic pathway. However, the critical mediators of adip...

journal_title：Molecular and cellular biology

authors： Wang ZV,Schraw TD,Kim JY,Khan T,Rajala MW,Follenzi A,Scherer PE

更新日期：2007-05-01 00:00:00

abstract：:IRE1-alpha is an integral membrane protein of the endoplasmic reticulum (ER) that is a key sensor in the cellular transcriptional response to stress in the ER. Upon induction of ER stress, IRE1-alpha is activated, resulting in the synthesis of the active form of the transcription factor XBP1 via IRE1-mediated splicing...

journal_title：Molecular and cellular biology

authors： Huang ZM,Tan T,Yoshida H,Mori K,Ma Y,Yen TS

更新日期：2005-09-01 00:00:00

abstract：:RNA editing in trypanosomes has been proposed to occur through transesterification or endonuclease cleavage and RNA ligation reactions. Both models involve a chimeric intermediate in which a guide RNA (gRNA) is joined through its 3' oligo(U) tail to an editing site of the corresponding mRNA. Velocity centrifugation of...

journal_title：Molecular and cellular biology

authors： Piller KJ,Decker CJ,Rusché LN,Sollner-Webb B

更新日期：1995-06-01 00:00:00

abstract：:Myelin membranes are sheet-like extensions of oligodendrocytes that can be considered membrane domains distinct from the cell's plasma membrane. Consistent with the polarized nature of oligodendrocytes, we demonstrate that transcytotic transport of the major myelin-resident protein proteolipid protein (PLP) is a key e...

journal_title：Molecular and cellular biology

authors： Baron W,Ozgen H,Klunder B,de Jonge JC,Nomden A,Plat A,Trifilieff E,de Vries H,Hoekstra D

更新日期：2015-01-01 00:00:00

abstract：:To determine the types of alterations in gene structure induced by DNA-alkylating agents, we analyzed the restriction enzyme cleavage patterns of adenine phosphoribosyltransferase gene sequences in mutant strains of Chinese hamster ovary cells deficient in this enzyme. Base pair changes as detected by loss of restrict...

journal_title：Molecular and cellular biology

authors： Meuth M,Arrand JE

更新日期：1982-11-01 00:00:00

abstract：:There are five members of the RFX family of transcription factors in mammals. While RFX5 plays a well-defined role in the immune system, the functions of RFX1 to RFX4 remain largely unknown. We have generated mice with a deletion of the Rfx3 gene. RFX3-deficient mice exhibit frequent left-right (LR) asymmetry defects ...

journal_title：Molecular and cellular biology

authors： Bonnafe E,Touka M,AitLounis A,Baas D,Barras E,Ucla C,Moreau A,Flamant F,Dubruille R,Couble P,Collignon J,Durand B,Reith W

更新日期：2004-05-01 00:00:00

abstract：:The transcription factor LFB1 (HNF1) was initially identified as a regulator of liver-specific gene expression in mammals. It interacts with the promoter element HP1, which is functionally conserved between mammals and amphibians, suggesting that a homologous factor, XLFB1, also exists in Xenopus laevis. To study the ...

journal_title：Molecular and cellular biology

authors： Bartkowski S,Zapp D,Weber H,Eberle G,Zoidl C,Senkel S,Klein-Hitpass L,Ryffel GU

更新日期：1993-01-01 00:00:00

abstract：:We have analyzed the 5'-flanking region of one of the genes coding for the human acute-phase protein, serum amyloid A (SAA). We found that SAA mRNA could be increased fivefold in transfected cells by treatment with phorbol 12-myristate 13-acetate (PMA). To analyze this observation further, we placed a 265-base-pair 5'...

journal_title：Molecular and cellular biology

authors： Edbrooke MR,Burt DW,Cheshire JK,Woo P

更新日期：1989-05-01 00:00:00

abstract：:In roughly 5% of cases of acute lymphoblastic leukemia, a chromosomal translocation leads to expression of the oncogenic protein E2A-PBX1. The N-terminal portion of E2A-PBX1, encoded by the E2A gene, is identical in sequence to the corresponding portion of the E proteins E12/E47 and includes transcriptional activation...

journal_title：Molecular and cellular biology

authors： Bayly R,Murase T,Hyndman BD,Savage R,Nurmohamed S,Munro K,Casselman R,Smith SP,LeBrun DP

更新日期：2006-09-01 00:00:00

abstract：:Multiple biological functions have been ascribed to the Ras-related G protein R-Ras. These include the ability to transform NIH 3T3 fibroblasts, the promotion of cell adhesion, and the regulation of apoptotic responses in hematopoietic cells. To investigate the signaling mechanisms responsible for these biological phe...

journal_title：Molecular and cellular biology

authors： Osada M,Tolkacheva T,Li W,Chan TO,Tsichlis PN,Saez R,Kimmelman AC,Chan AM

更新日期：1999-09-01 00:00:00

abstract：:TFIID is a multiprotein complex consisting of the TATA box binding protein and multiple tightly associated proteins (TAFIIs) that are required for transcription by selected activators. We previously reported cloning and partial characterization of human TAFII130 (hTAFII130). The central domain of hTAFII130 contains fo...

journal_title：Molecular and cellular biology

authors： Saluja D,Vassallo MF,Tanese N

更新日期：1998-10-01 00:00:00

abstract：:Nutritional excess and/or obesity represent well-known predisposition factors for the development of non-insulin-dependent diabetes mellitus (NIDDM). However, molecular links between obesity and NIDDM are only beginning to emerge. Here, we demonstrate that nutrients suppress phosphatidylinositol 3 (PI3)-kinase/Akt sig...

journal_title：Molecular and cellular biology

authors： Tzatsos A,Kandror KV

更新日期：2006-01-01 00:00:00

abstract：:The chromatin structure of TDH3, one of three genes encoding glyceraldehyde phosphate dehydrogenases in Saccharomyces cerevisiae, was analyzed by nuclease digestion. A large hypersensitive region was found at the TDH3 promoter extending from the RNA initiation site at position -40 to position -560. This hypersensitive...

journal_title：Molecular and cellular biology

authors： Pavlović B,Hörz W

更新日期：1988-12-01 00:00:00

abstract：:We have examined the possible role of adenosine 3',5'-phosphate (cAMP) in functions associated with the plasma membranes of Saccharomyces cerevisiae. Purified membranes from this source contained an adenylate cyclase which was insensitive to activation by fluoride or guanine nucleotides, only weakly responsive to chan...

journal_title：Molecular and cellular biology

authors： Jaynes PK,McDonough JP,Mahler HR

更新日期：1982-12-01 00:00:00

abstract：:We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridization. To isolate a rec...

journal_title：Molecular and cellular biology

authors： Milbrandt JD,Azizkhan JC,Greisen KS,Hamlin JL

更新日期：1983-07-01 00:00:00

abstract：:Mutations in the Drosophila mus308 gene confer specific hypersensitivity to DNA-cross-linking agents as a consequence of defects in DNA repair. The mus308 gene is shown here to encode a 229-kDa protein in which the amino-terminal domain contains the seven conserved motifs characteristic of DNA and RNA helicases and th...

journal_title：Molecular and cellular biology

authors： Harris PV,Mazina OM,Leonhardt EA,Case RB,Boyd JB,Burtis KC

更新日期：1996-10-01 00:00:00