Identification of a novel isoform of the retinoic acid receptor gamma expressed in the mouse embryo.

abstract：:We investigated the role of RNA polymerase II (pol II) carboxy-terminal domain (CTD) phosphorylation in pre-mRNA processing coupled and uncoupled from transcription in Xenopus oocytes. Inhibition of CTD phosphorylation by the kinase inhibitors 5,6-dichloro-1beta-D-ribofuranosyl-benzimidazole and H8 blocked transcripti...

journal_title：Molecular and cellular biology

authors： Bird G,Zorio DA,Bentley DL

更新日期：2004-10-01 00:00:00

abstract：:Endonuclease decay of nonsense-containing beta-globin mRNA in erythroid cells generates 5'-truncated products that were reported previously to have a cap or caplike structure. We confirmed that this 5' modification is indistinguishable from the cap on full-length mRNA, and Western blotting, immunoprecipitation, and ac...

journal_title：Molecular and cellular biology

authors： Otsuka Y,Kedersha NL,Schoenberg DR

更新日期：2009-04-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae CYC1 gene produces mRNA with more than 20 different 5' ends. A derivative of the CYC1 gene (CYC1-157) was constructed with a deletion of a portion of the CYC1 5'-noncoding region, which includes the sites at which many of the CYC1 mRNAs 5' ends map. A 54-mer double-stranded o...

journal_title：Molecular and cellular biology

authors： McNeil JB,Smith M

更新日期：1985-12-01 00:00:00

abstract：:HIRA-like (Hir) proteins are evolutionarily conserved and are implicated in the assembly of repressive chromatin. In Saccharomyces cerevisiae, Hir proteins contribute to the function of centromeres. However, S. cerevisiae has point centromeres that are structurally different from the complex centromeres of metazoans. ...

journal_title：Molecular and cellular biology

authors： Blackwell C,Martin KA,Greenall A,Pidoux A,Allshire RC,Whitehall SK

更新日期：2004-05-01 00:00:00

abstract：:Polycomb (Pc) is part of a Pc group (PcG) protein complex that is involved in repression of gene activity during Drosophila and vertebrate development. To identify proteins that interact with vertebrate Pc homologs, we performed two-hybrid screens with Xenopus Pc (XPc) and human Pc 2 (HPC2). We find that the C-termina...

journal_title：Molecular and cellular biology

authors： Sewalt RG,Gunster MJ,van der Vlag J,Satijn DP,Otte AP

更新日期：1999-01-01 00:00:00

abstract：:p27(Kip1) is a member of the Cip-Kip family of cyclin-dependent kinase (Cdk) inhibitors that binds to cyclin-Cdk complexes and inhibits their catalytic activity in response to antiproliferative stimuli. p27(Kip1) is regulated by several posttranscriptional mechanisms, including subcellular localization. We have identi...

journal_title：Molecular and cellular biology

authors： Smitherman M,Lee K,Swanger J,Kapur R,Clurman BE

更新日期：2000-08-01 00:00:00

abstract：:We have isolated and subcloned three separate segments of human DNA which share strong sequence homology with a previously sequenced gene encoding a type I keratin, K14 (50 kilodaltons). Restriction endonuclease mapping has demonstrated that these three genes are tightly linked chromosomally, whereas the K14 gene appe...

journal_title：Molecular and cellular biology

authors： RayChaudhury A,Marchuk D,Lindhurst M,Fuchs E

更新日期：1986-02-01 00:00:00

abstract：:During investigations on the regulation of the Pax-6 gene, we characterized a cDNA from quail neuroretina showing a 5' untranslated region distinct from that previously described and initiated from an internal promoter. Using RNase protection and primer extension mapping, we localized this second quail Pax-6 promoter,...

journal_title：Molecular and cellular biology

authors： Plaza S,Dozier C,Turque N,Saule S

更新日期：1995-06-01 00:00:00

abstract：:The glucose-activated transcription factor carbohydrate response element binding protein (ChREBP) induces the expression of hepatic glycolytic and lipogenic genes. The farnesoid X receptor (FXR) is a nuclear bile acid receptor controlling bile acid, lipid, and glucose homeostasis. FXR negatively regulates hepatic glyc...

journal_title：Molecular and cellular biology

authors： Caron S,Huaman Samanez C,Dehondt H,Ploton M,Briand O,Lien F,Dorchies E,Dumont J,Postic C,Cariou B,Lefebvre P,Staels B

更新日期：2013-06-01 00:00:00

abstract：:Transcription of the c-rel proto-oncogene was induced transiently when resting mouse NIH 3T3 fibroblasts were stimulated with serum or phorbol-12-myristate-13-acetate. Addition of cycloheximide increased the steady-state levels of c-rel mRNA. These results indicate that c-rel is another member of the early-response ge...

journal_title：Molecular and cellular biology

authors： Bull P,Hunter T,Verma IM

更新日期：1989-11-01 00:00:00

abstract：:T-lymphocyte stimulation requires activation of several protein kinases, including the major phorbol ester receptor protein kinase C (PKC), ultimately leading to induction of lymphokines, such as interleukin-2 (IL-2). The revelant PKC isoforms which are involved in the activation cascades of nuclear transcription fact...

journal_title：Molecular and cellular biology

authors： Baier-Bitterlich G,Uberall F,Bauer B,Fresser F,Wachter H,Grunicke H,Utermann G,Altman A,Baier G

更新日期：1996-04-01 00:00:00

abstract：:Several ETS transcription factors, including ELF4/MEF, can function as oncogenes in murine cancer models and are overexpressed in human cancer. We found that Elf4/Mef activates Mdm2 expression; thus, lack of or knockdown of Elf4/Mef reduces Mdm2 levels in mouse embryonic fibroblasts (mef's), leading to enhanced p53 pr...

journal_title：Molecular and cellular biology

authors： Sashida G,Liu Y,Elf S,Miyata Y,Ohyashiki K,Izumi M,Menendez S,Nimer SD

更新日期：2009-07-01 00:00:00

abstract：:We studied RNA polymerase I transcription in cells transfected with a plasmid, prHuTK, containing the herpes simplex virus tk gene fused to a human rRNA promoter. Primer extension analysis of tk RNA isolated from COS cells transfected with prHuTK reveals that transcription from the RNA polymerase I promoter is highly ...

journal_title：Molecular and cellular biology

authors： Smale ST,Tjian R

更新日期：1985-02-01 00:00:00

abstract：:To characterize further the regulatory mechanism modulating the expression of the Saccharomyces cerevisiae ARG3 gene, i.e., the specific repression by arginine and the general amino acid control, we analyzed by deletion the region upstream of that gene, determined the nucleotide sequence of operator-constitutive-like ...

journal_title：Molecular and cellular biology

authors： Crabeel M,Huygen R,Verschueren K,Messenguy F,Tinel K,Cunin R,Glansdorff N

更新日期：1985-11-01 00:00:00

abstract：:Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1) signa...

journal_title：Molecular and cellular biology

authors： Llobet-Navas D,Rodriguez-Barrueco R,de la Iglesia-Vicente J,Olivan M,Castro V,Saucedo-Cuevas L,Marshall N,Putcha P,Castillo-Martin M,Bardot E,Ezhkova E,Iavarone A,Cordon-Cardo C,Silva JM

更新日期：2014-12-01 00:00:00

abstract：:We have partially purified a nuclear protein (PPT) from Physarum polycephalum that binds to the extrachromosomal ribosomal DNA telomeres of this acellular slime mold. Binding is specific for the (T2AG3)n telomere repeats, as evidenced by nitrocellulose filter binding assays, by gel mobility shift assays with both DNA ...

journal_title：Molecular and cellular biology

authors： Coren JS,Epstein EM,Vogt VM

更新日期：1991-04-01 00:00:00

abstract：:We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a recipro...

journal_title：Molecular and cellular biology

authors： Klein HL,Petes TD

更新日期：1984-02-01 00:00:00

abstract：:Signaling via the pre-T-cell receptor (pre-TCR), along with associated signals from Notch and chemokine receptors, regulates the β-selection checkpoint that operates on CD4(-) CD8(-) doubly negative (DN) thymocytes. Since many hematopoietic malignancies arise at the immature developmental stages of lymphocytes, unders...

journal_title：Molecular and cellular biology

authors： Trampont PC,Zhang L,Giles AJ,Walk SF,Gu JJ,Pendergast AM,Ravichandran KS

更新日期：2015-04-01 00:00:00

abstract：:The ligand-binding domains of thyroid hormone (L-triiodothyronine [T3]) receptors (T3Rs), all-trans retinoic acid (RA) receptors (RARs), and 9-cis RA receptors (RARs and RXRs) contain a series of heptad motifs thought to be important for dimeric interactions. Using a chimera containing amino acids 120 to 392 of chicke...

journal_title：Molecular and cellular biology

authors： Casanova J,Helmer E,Selmi-Ruby S,Qi JS,Au-Fliegner M,Desai-Yajnik V,Koudinova N,Yarm F,Raaka BM,Samuels HH

更新日期：1994-09-01 00:00:00

abstract：:The NK-2 homeobox genes have been shown to play critical roles in the development of specific organs and tissues. Nkx2.6 is a member of the NK-2 homeobox gene family and is most closely related to the Drosophila tinman gene. Nkx2.6 is expressed in the caudal pharyngeal pouches, the caudal heart progenitors, the sinus ...

journal_title：Molecular and cellular biology

authors： Tanaka M,Yamasaki N,Izumo S

更新日期：2000-04-01 00:00:00

abstract：:Temperature-sensitive mutants of TFIIB that are defective for essential interactions were isolated. One mutation (G204D) results in disruption of a protein-protein contact between TFIIB and TATA binding protein (TBP), while the other (K272I) disrupts an interaction between TFIIB and DNA. The TBP gene was mutagenized, ...

journal_title：Molecular and cellular biology

authors： Buratowski RM,Downs J,Buratowski S

更新日期：2002-12-01 00:00:00

abstract：:The CBP/p300 family of proteins comprises related acetyltransferases that coactivate signal-responsive transcription. Recent evidence suggests that p300/CBP may also interact directly with complexes that mediate different aspects of DNA metabolism such as replication and repair. In this report, we show that loss of dC...

journal_title：Molecular and cellular biology

authors： Smolik S,Jones K

更新日期：2007-01-01 00:00:00

abstract：:Pref-1/Dlk1 is made as an epidermal growth factor (EGF) repeat-containing transmembrane protein but is cleaved by tumor necrosis factor alpha converting enzyme (TACE) to generate a biologically active soluble form. Soluble Pref-1 inhibits adipocyte differentiation through the activation of extracellular signal-regulat...

journal_title：Molecular and cellular biology

authors： Wang Y,Zhao L,Smas C,Sul HS

更新日期：2010-07-01 00:00:00

abstract：:PC12 cells treated with nerve growth factor (NGF) or infected with Rous sarcoma virus differentiate into sympathetic, neuronlike cells. To compare the differentiation programs induced by NGF and v-src, we have established a PC12 cell line expressing a temperature-sensitive v-src protein. The v-src-expressing PC12 cell...

journal_title：Molecular and cellular biology

authors： Thomas SM,Hayes M,D'Arcangelo G,Armstrong RC,Meyer BE,Zilberstein A,Brugge JS,Halegoua S

更新日期：1991-09-01 00:00:00

abstract：:The K-fgf/hst oncogene encodes a secreted growth factor of the fibroblast growth factor (FGF) family. The ability of K-fgf-transformed cells to grow in soft agar and in serum-free medium is inhibited by anti-K-FGF neutralizing antibodies, consistent with an autocrine mechanism of transformation. The transformed proper...

journal_title：Molecular and cellular biology

authors： Talarico D,Basilico C

更新日期：1991-02-01 00:00:00

abstract：:We have previously proposed that IQGAP1, an effector of Rac1 and Cdc42, negatively regulates cadherin-mediated cell-cell adhesion by interacting with beta-catenin and by causing the dissociation of alpha-catenin from cadherin-beta-catenin-alpha-catenin complexes and that activated Rac1 and Cdc42 positively regulate ca...

journal_title：Molecular and cellular biology

authors： Fukata M,Nakagawa M,Itoh N,Kawajiri A,Yamaga M,Kuroda S,Kaibuchi K

更新日期：2001-03-01 00:00:00

abstract：:We identified Ras guanine-releasing protein 3 (RasGRP3) as a guanine exchange factor expressed in blood vessels via an embryonic stem (ES) cell-based gene trap screen to identify novel vascular genes. RasGRP3 is expressed in embryonic blood vessels, down-regulated in mature adult vessels, and reexpressed in newly form...

journal_title：Molecular and cellular biology

authors： Roberts DM,Anderson AL,Hidaka M,Swetenburg RL,Patterson C,Stanford WL,Bautch VL

更新日期：2004-12-01 00:00:00

abstract：:MicroRNA 155 (miR-155) is an oncomir, generated as a noncoding RNA from the BIC gene whose promoter activity is mainly controlled via activation protein 1 (AP-1) and NF-κB transcription factors. We found that the expression levels of miR-155 and programmed cell death 4 (Pdcd4) exhibit inverse relationships in tongue c...

journal_title：Molecular and cellular biology

authors： Zargar S,Tomar V,Shyamsundar V,Vijayalakshmi R,Somasundaram K,Karunagaran D

更新日期：2019-03-01 00:00:00

abstract：:The LAR family protein tyrosine phosphatases (PTPs), including LAR, PTP delta, and PTP sigma, are transmembrane proteins composed of a cell adhesion molecule-like ectodomain and two cytoplasmic catalytic domains: active D1 and inactive D2. We performed a yeast two-hybrid screen with the first catalytic domain of PTP s...

journal_title：Molecular and cellular biology

authors： Wallace MJ,Fladd C,Batt J,Rotin D

更新日期：1998-05-01 00:00:00

abstract：:The mitochondrial genome of trypanosomes is composed of thousands of topologically interlocked circular DNA molecules that form the kinetoplast DNA (kDNA). Most genes encoded by the kDNA require a posttranscriptional modification process called RNA editing to form functional mRNAs. Here, we show that alternative editi...

journal_title：Molecular and cellular biology

authors： Ochsenreiter T,Anderson S,Wood ZA,Hajduk SL

更新日期：2008-09-01 00:00:00