Phenotypic characterization of the murine Nkx2.6 homeobox gene by gene targeting.

abstract：:Although both osteoblasts and adipocytes have a common origin, i.e., mesenchymal cells, the molecular mechanisms that define the direction of two different lineages are presently unknown. In this study, we investigated the role of a transcription factor, CCAAT/enhancer binding protein beta (C/EBPbeta), and its isoform...

journal_title：Molecular and cellular biology

authors： Hata K,Nishimura R,Ueda M,Ikeda F,Matsubara T,Ichida F,Hisada K,Nokubi T,Yamaguchi A,Yoneda T

更新日期：2005-03-01 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is a conserved proofreading mechanism that protects eukaryotic cells from the potentially deleterious effects of truncated proteins. Studies of Saccharomyces cerevisiae imply that NMD is a predominantly cytoplasmic decay pathway, while studies of mammalian systems suggest that decay ...

journal_title：Molecular and cellular biology

authors： Maderazo AB,Belk JP,He F,Jacobson A

更新日期：2003-02-01 00:00:00

abstract：:We have identified an essential cis element in the proximal promoter region of the rat carbamyl phosphate synthetase I (CPSI) gene that is requisite for promoter activity in liver nuclear extracts. Excess synthetic oligonucleotides specifying this region abolished promoter-dependent in vitro transcription. We show tha...

journal_title：Molecular and cellular biology

authors： Howell BW,Lagacé M,Shore GC

更新日期：1989-07-01 00:00:00

abstract：:A 249-nucleotide coding region instability determinant (CRD) destabilizes c-myc mRNA. Previous experiments identified a CRD-binding protein (CRD-BP) that appears to protect the CRD from endonuclease cleavage. However, it was unclear why a CRD-BP is required to protect a well-translated mRNA whose coding region is cove...

journal_title：Molecular and cellular biology

authors： Lemm I,Ross J

更新日期：2002-06-01 00:00:00

abstract：:Selenoprotein mRNAs are potential targets for degradation via nonsense-mediated decay due to the presence of in-frame UGA codons that can be decoded as either selenocysteine or termination codons. When UGA decoding is inefficient, as occurs when selenium is limiting, termination occurs at these positions. Based on the...

journal_title：Molecular and cellular biology

authors： Squires JE,Stoytchev I,Forry EP,Berry MJ

更新日期：2007-11-01 00:00:00

abstract：:Binding sites for six distinct nuclear factors on the 75-base-pair repeat of the Moloney murine leukemia virus enhancer have been identified by an electrophoretic mobility shift assay combined with methylation interference. Three of these factors, found in WEHI 231 nuclear extracts, which we have named LVa, LVb, and L...

journal_title：Molecular and cellular biology

authors： Speck NA,Baltimore D

更新日期：1987-03-01 00:00:00

abstract：:TRF1 is a component of the shelterin complex at mammalian telomeres; however, a role for TRF1 in telomere biology in the context of the organism is unclear. In this study, we generated mice with transgenic TRF1 expression targeted to epithelial tissues (K5TRF1 mice). K5TRF1 mice have shorter telomeres in the epidermis...

journal_title：Molecular and cellular biology

authors： Muñoz P,Blanco R,de Carcer G,Schoeftner S,Benetti R,Flores JM,Malumbres M,Blasco MA

更新日期：2009-03-01 00:00:00

abstract：:Two alternative exons, BEK and K-SAM, code for part of the ligand binding site of fibroblast growth factor receptor 2. Splicing of these exons is mutually exclusive, and the choice between them is made in a tissue-specific manner. We identify here pre-mRNA sequences involved in controlling splicing of the K-SAM exon. ...

journal_title：Molecular and cellular biology

authors： Del Gatto F,Breathnach R

更新日期：1995-09-01 00:00:00

abstract：:In this report, we document an unusual mode of tissue-enriched gene expression that is primarily mediated by alternative and inefficient splicing. We have analyzed posttranscriptional regulation of the Drosophila erect wing gene, which provides a vital neuronal function and is essential for the formation of certain mu...

journal_title：Molecular and cellular biology

authors： Koushika SP,Soller M,DeSimone SM,Daub DM,White K

更新日期：1999-06-01 00:00:00

abstract：:B-myc is a recently described myc gene whose product has not been functionally characterized. The predicted product of B-myc is a 168-amino-acid protein with extensive homology to the c-Myc amino-terminal region, previously shown to contain a transcriptional activation domain. We hypothesized that B-Myc might also fun...

journal_title：Molecular and cellular biology

authors： Resar LM,Dolde C,Barrett JF,Dang CV

更新日期：1993-02-01 00:00:00

abstract：:Cells of the human embryonal carcinoma line NEC14 proliferate as densely packed clusters consisting of small, polygonal stem cells and do not express a detectable level of fibronectin (FN). Upon induction of differentiation by treatment with N,N'-hexamethylene bisacetamide (HMBA), the level of FN mRNA increased steepl...

journal_title：Molecular and cellular biology

authors： Suzuki M,Oda E,Nakajima T,Sekiya S,Oda K

更新日期：1998-05-01 00:00:00

abstract：:In the early phase of bone damage, low concentrations of the cytokine tumor necrosis factor alpha (TNF-α) favor osteoblast differentiation. In contrast, chronic high doses of the same cytokine contribute to bone loss, demonstrating opposite effects depending on its concentration and on the time of exposure. In the bon...

journal_title：Molecular and cellular biology

authors： Daniele S,Natali L,Giacomelli C,Campiglia P,Novellino E,Martini C,Trincavelli ML

更新日期：2017-03-31 00:00:00

abstract：:The Caenorhabditis elegans cuticle collagens are encoded by a multigene family of between 50 and 100 members and are the major component of the nematode cuticular exoskeleton. They are synthesized in the hypodermis prior to secretion and incorporation into the cuticle and exhibit complex patterns of spatial and tempor...

journal_title：Molecular and cellular biology

authors： Gilleard JS,Barry JD,Johnstone IL

更新日期：1997-04-01 00:00:00

abstract：:P bodies are cytoplasmic RNA granules containing the Dcp1-Dcp2 decapping enzymes where mRNA decay can occur. Here, we describe the characterization of P bodies in the fission yeast Schizosaccharomyces pombe. Most information on the property and function of P bodies stems from studies in the distantly related budding y...

journal_title：Molecular and cellular biology

authors： Wang CY,Chen WL,Wang SW

更新日期：2013-03-01 00:00:00

abstract：:Sophisticated models for the regulation of mitotic entry are lacking for human cells. Inactivating human cyclin A/Cdk2 complexes through diverse approaches delays mitotic entry and promotes inhibitory phosphorylation of Cdk1 on tyrosine 15, a modification performed by Wee1. We show here that cyclin A/Cdk2 complexes ph...

journal_title：Molecular and cellular biology

authors： Li C,Andrake M,Dunbrack R,Enders GH

更新日期：2010-01-01 00:00:00

abstract：:The p53 tumor suppressor gene product is a sequence-specific DNA-binding protein that is necessary for the G1 arrest of many cell types. Consistent with its role as a cell cycle checkpoint factor, p53 has been shown to be capable of both transcriptional activation and repression. Here we show a new potential role for ...

journal_title：Molecular and cellular biology

authors： Miller SD,Farmer G,Prives C

更新日期：1995-12-01 00:00:00

abstract：:The chaperone-related AAA ATPase Cdc48 (p97/VCP in higher eukaryotes) segregates ubiquitylated proteins for subsequent degradation by the 26S proteasome or for nonproteolytic fates. The specific outcome of Cdc48 activity is controlled by the evolutionary conserved cofactors Ufd2 and Ufd3, which antagonistically regula...

journal_title：Molecular and cellular biology

authors： Böhm S,Lamberti G,Fernández-Sáiz V,Stapf C,Buchberger A

更新日期：2011-04-01 00:00:00

abstract：:Expression of the anaphase-promoting complex/cyclosome (APC/C) inhibitor Emi1 is required for the accumulation of APC/C substrates crucial for DNA synthesis and mitotic entry. We show that in vivo Emi1 expression correlates with the proliferative status of the cellular compartment and that cells lacking Emi1 undergo c...

journal_title：Molecular and cellular biology

authors： Verschuren EW,Ban KH,Masek MA,Lehman NL,Jackson PK

更新日期：2007-11-01 00:00:00

abstract：:The fibroblast integrin alpha11beta1 is a key receptor for fibrillar collagens. To study the potential function of alpha11 in vivo, we generated a null allele of the alpha11 gene. Integrin alpha11(-/-) mice are viable and fertile but display dwarfism with increased mortality, most probably due to severely defective in...

journal_title：Molecular and cellular biology

authors： Popova SN,Barczyk M,Tiger CF,Beertsen W,Zigrino P,Aszodi A,Miosge N,Forsberg E,Gullberg D

更新日期：2007-06-01 00:00:00

abstract：:A variety of genes, including c-myc, are activated by chemical toxicants in vivo and in vitro. Although enforced c-myc expression induces apoptosis after withdrawing survival factors, it is not clear if activation of the endogenous c-myc gene is an apoptotic signal after toxicant exposure. The renal tubular epithelium...

journal_title：Molecular and cellular biology

authors： Zhan Y,Cleveland JL,Stevens JL

更新日期：1997-11-01 00:00:00

abstract：:Two adjacent fragments of genomic DNA spanning the gene for CAD, which encodes the first three enzymes of UMP biosynthesis, were cloned from a mutant Syrian hamster cell line containing multiple copies of this gene. The mutant was selected for resistance to N-(phosphonacetyl)-L-aspartate, a potent and specific inhibit...

journal_title：Molecular and cellular biology

authors： Padgett RA,Wahl GM,Stark GR

更新日期：1982-03-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) are unipotent germ cells that are at the foundation of spermatogenesis and male fertility. However, the underlying molecular mechanisms governing SSC stemness and growth properties remain elusive. We have recently identified chromodomain helicase/ATPase DNA binding protein 1-like (Chd1...

journal_title：Molecular and cellular biology

authors： Liu SS,Maguire EM,Bai YS,Huang L,Liu Y,Xu L,Fauzi I,Zhang SQ,Xiao Q,Ma NF

更新日期：2019-02-04 00:00:00

abstract：:The establishment of epigenetic marks, such as methylation on histone tails, is mechanistically linked to RNA polymerase II within active genes. To explore the interplay between these modifications in transcribed noncoding as well as coding sequences, we analyzed epigenetic modification and chromatin structure at high...

journal_title：Molecular and cellular biology

authors： Kim A,Kiefer CM,Dean A

更新日期：2007-02-01 00:00:00

abstract：:Translation is a fundamental step in gene expression, and translational control is exerted in many developmental processes. Most eukaryotic mRNAs are translated by a cap-dependent mechanism, which requires recognition of the 5'-cap structure of the mRNA by eukaryotic translation initiation factor 4E (eIF4E). eIF4E act...

journal_title：Molecular and cellular biology

authors： Hernández G,Miron M,Han H,Liu N,Magescas J,Tettweiler G,Frank F,Siddiqui N,Sonenberg N,Lasko P

更新日期：2013-08-01 00:00:00

abstract：:TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. In vivo, the TACC2 gene is expressed in various splice forms predominantly in postmitotic tissues, including heart, muscle, kidney, and brain. Studies of human...

journal_title：Molecular and cellular biology

authors： Schuendeln MM,Piekorz RP,Wichmann C,Lee Y,McKinnon PJ,Boyd K,Takahashi Y,Ihle JN

更新日期：2004-07-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the MET25 gene encodes O-acetylhomoserine sulfhydrylase. Synthesis of this enzyme is repressed by the presence of S-adenosylmethionine (AdoMet) in the growth medium. We identified cis elements required for MET25 expression by analyzing small deletions in the MET25 promoter region. The resu...

journal_title：Molecular and cellular biology

authors： Thomas D,Cherest H,Surdin-Kerjan Y

更新日期：1989-08-01 00:00:00

abstract：:Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1) signa...

journal_title：Molecular and cellular biology

authors： Llobet-Navas D,Rodriguez-Barrueco R,de la Iglesia-Vicente J,Olivan M,Castro V,Saucedo-Cuevas L,Marshall N,Putcha P,Castillo-Martin M,Bardot E,Ezhkova E,Iavarone A,Cordon-Cardo C,Silva JM

更新日期：2014-12-01 00:00:00

abstract：:The GGAs (Golgi-localized, gamma-ear-containing, ARF-binding proteins) are a family of multidomain adaptor proteins involved in protein sorting at the trans-Golgi network of eukaryotic cells. Here we present results from a functional characterization of the two Saccharomyces cerevisiae GGAs, Gga1p and Gga2p. We show t...

journal_title：Molecular and cellular biology

authors： Mullins C,Bonifacino JS

更新日期：2001-12-01 00:00:00

abstract：:We document a biphasic effect of Rac2 on the activation and inhibition of PLD2. Cells overexpressing Rac2 and PLD2 simultaneously show a robust initial (<10 min) response toward a chemoattractant that is later (>30 min) greatly diminished over PLD2-only controls. The first phase is due to the presence of a Rac2-PLD2 p...

journal_title：Molecular and cellular biology

authors： Peng HJ,Henkels KM,Mahankali M,Marchal C,Bubulya P,Dinauer MC,Gomez-Cambronero J

更新日期：2011-06-01 00:00:00

abstract：:Nucleoporins mediate transport of macromolecules across the nuclear pore complex, yet the function of many individual nucleoporins is largely unresolved. To address this question, we depleted cells of the cytoplasmic nucleoporins Nup214/CAN and Nup358/RanBP2 by RNA interference. Depletion of Nup214 resulted in codeple...

journal_title：Molecular and cellular biology

authors： Hutten S,Kehlenbach RH

更新日期：2006-09-01 00:00:00