Abstract:
:The major cytoskeletal actin gene of Drosophila melanogaster, the actin 5C gene, has two promoters, the distal one of which controls synthesis of actin in a tissue- and developmental stage-specific manner. This very strong promoter has widely been used for expression of heterologous genes in cultured cells. To locate functional regulatory elements in this distal promoter, mutants of the promoter were fused to the bacterial chloramphenicol acetyltransferase gene and assayed for transient expression activity in cultured Drosophila embryonic Schneider line 2 cells. The results showed that the upstream end of the promoter extends to 522 bp from the transcription start site. In addition, there are two remote activating regions about 2 kb upstream. Between -522 and -379 are two regions that exert a strong negative effect. Downstream from these negative regions are at least six positive regions and a TATA element. The strongest positive determinant of the promoter was identified at -320 as AAAATGTG by footprinting and by a replacement experiment. When the relevant region was replaced by a synthetic sequence containing this element in a random context, the transient expression activity was restored. The sequence TGTATG located at -355 was also identified as a positive element by a similar replacement approach. Apparently the very high activity of this promoter is the result of the combined activities of multiple factors.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Chung YT,Keller EBdoi
10.1128/mcb.10.12.6172subject
Has Abstractpub_date
1990-12-01 00:00:00pages
6172-80issue
12eissn
0270-7306issn
1098-5549journal_volume
10pub_type
杂志文章abstract::Human neuroblastoma cells often carry amplified DNA encompassing the gene N-myc. Amplified N-myc has been found localized in "double minutes" in direct tumor cell preparations. In contrast, later passages carried amplified N-myc almost exclusively within a single homogeneously staining chromosomal region located at a ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.9.11.4903
更新日期:1989-11-01 00:00:00
abstract::Histone modifications occur in precise patterns and are proposed to signal the recruitment of effector molecules that profoundly impact chromatin structure, gene regulation, and cell cycle events. The linked modifications serine 10 phosphorylation and lysine 14 acetylation on histone H3 (H3S10phK14ac), modifications c...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01457-07
更新日期:2008-04-01 00:00:00
abstract::The single-copy gene encoding the alpha subunit of glycoprotein hormones is expressed in the pituitaries of all mammals and in the placentas of only primates and horses. We have systematically analyzed the promoter-regulatory elements of the human and bovine alpha-subunit genes to elucidate the molecular mechanisms un...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.9.11.5113
更新日期:1989-11-01 00:00:00
abstract::The autonomously replicating rRNA genes (rDNA) in the somatic nucleus of Tetrahymena thermophila are maintained at a copy number of approximately 10(4) per nucleus. A mutant in which the replication properties of this molecule were altered was isolated and characterized. This mutation of inbred strain C3, named rmm4, ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.9.2.452
更新日期:1989-02-01 00:00:00
abstract::Krüppel-like factors 3 and 8 (KLF3 and KLF8) are highly related transcriptional regulators that bind to similar sequences of DNA. We have previously shown that in erythroid cells there is a regulatory hierarchy within the KLF family, whereby KLF1 drives the expression of both the Klf3 and Klf8 genes and KLF3 in turn r...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00074-13
更新日期:2013-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.10.4.1754
更新日期:1990-04-01 00:00:00
abstract::In Chlamydomonas reinhardi the chloroplast DNA (ch;DNA) of mating type plus cells undergoes cyclical methylation and demethylation during the life cycle. Methylation occurs during gametogenesis, and fully differentiated gametes can be dedifferentiated back to vegetative cells which contain nonmethylated chlDNA by the ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.4.10.2103
更新日期:1984-10-01 00:00:00
abstract::The extracellular matrix (ECM) promotes tissue morphogenesis, cell migration, and the differentiation of a variety of cell types. However, the mechanisms by which ECM causes differentiated gene expression have been unknown. In this report, we show that culturing the hepatocyte-derived cell line H2.35 on an ECM gel cha...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.11.9.4405
更新日期:1991-09-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.11.6752
更新日期:1993-11-01 00:00:00
abstract::Oncostatin M is a polypeptide cytokine, produced by normal and malignant hematopoietic cells, that has several in vitro activities, including the ability to inhibit growth of cultured carcinoma cells. Here we present a structural and functional comparison of two oncostatin M-related proteins (Mr 36,000 and 32,000) sec...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.10.5.1882
更新日期:1990-05-01 00:00:00
abstract::During mammalian dosage compensation, one of two X-chromosomes in female cells is inactivated. The choice of which X is silenced can be imprinted or stochastic. Although genetic loci influencing the choice decision have been identified, the primary marks for imprinting and random selection remain undefined. Here, we e...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.26.6.2109-2117.2006
更新日期:2006-03-01 00:00:00
abstract::The erb A oncogene is a dominant negative allele of a thyroid hormone receptor gene and acts in the cancer cell by encoding a transcriptional repressor. We demonstrate here that the DNA sequence recognition properties of the oncogenic form of the erb A protein are significantly altered from those of the normal thyroid...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.4.2366
更新日期:1993-04-01 00:00:00
abstract::Our objective was to establish an experimental model of a self-sustained and bistable extracellular signal-regulated kinase 1/2 (ERK1/2) signaling process. A single stimulation of cells with cytokines causes rapid ERK1/2 activation, which returns to baseline in 4 h. Repeated stimulation leads to sustained activation o...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01003-09
更新日期:2010-04-01 00:00:00
abstract::Welander distal myopathy (WDM) is a muscle dystrophy characterized by adult-onset distal muscle weakness, prevalently impacting the distal long extensors of the hands and feet. WDM is an autosomal dominant disorder caused by a missense mutation (c.1362G>A; p.E384K) in the TIA1 (T-cell intracellular antigen 1) gene, wh...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00299-18
更新日期:2018-12-11 00:00:00
abstract::Eukaryotic viruses have devised numerous strategies to downregulate activity of the interferon-induced, double-stranded (dsRNA)-activated protein kinase (referred to as p68 on the basis of its Mr of 68,000 in human cells). Viruses must exert this control to avoid extensive phosphorylation of the alpha subunit of eukar...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.11.11.5497
更新日期:1991-11-01 00:00:00
abstract::The UvsW protein of bacteriophage T4 is involved in many aspects of phage DNA metabolism, including repair, recombination, and recombination-dependent replication. UvsW has also been implicated in the repression of origin-dependent replication at late times of infection, when UvsW is normally synthesized. Two well-cha...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.21.8.2706-2715.2001
更新日期:2001-04-01 00:00:00
abstract::Histone H3 lysine 9 (H3K9) methylation has broad roles in transcriptional repression, gene silencing, maintenance of heterochromatin, and epigenetic inheritance of heterochromatin. Using Xenopus laevis oocytes, we have previously shown that targeting G9a, an H3K9 histone methyltransferase, to chromatin increases H3K9 ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00948-06
更新日期:2006-09-01 00:00:00
abstract::The murine Igh locus has a 3' regulatory region (3' RR) containing four enhancers (hs3A, hs1,2, hs3B, and hs4) at DNase I-hypersensitive sites. The 3' RR exerts long-range effects on class switch recombination (CSR) to several isotypes through its control of germ line transcription. By measuring levels of acetylated h...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.25.4.1511-1525.2005
更新日期:2005-02-01 00:00:00
abstract::The accumulation of assembled holoenzymes composed of regulatory D-type cyclins and their catalytic partner, cyclin-dependent kinase 4 (cdk4), is rate limiting for progression through the G1 phase of the cell cycle in mammalian fibroblasts. Both the synthesis and assembly of D-type cyclins and cdk4 depend upon serum s...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.14.4.2713
更新日期:1994-04-01 00:00:00
abstract::To determine if Neu is dominant over transforming growth factor beta (TGF-beta), we crossed mouse mammary tumor virus (MMTV)-Neu mice with MMTV-TGF-beta1(S223/225) mice expressing active TGF-beta1 in the mammary gland. Bigenic (NT) and Neu-induced mammary tumors developed with a similar latency. The bigenic tumors and...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.23.23.8691-8703.2003
更新日期:2003-12-01 00:00:00
abstract::Histone methylation at H3K4 and H3K36 is commonly associated with genes actively transcribed by RNA polymerase II (RNAPII) and is catalyzed by Saccharomyces cerevisiae Set1 and Set2, respectively. Here we report that both methyltransferases can be UV cross-linked to RNA in vivo High-throughput sequencing of the bound ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00165-17
更新日期:2017-06-29 00:00:00
abstract::A previously uncharacterized Saccharomyces cerevisiae gene, FAL1, was found by sequence comparison as a homolog of the eukaryotic translation initiation factor 4A (eIF4A). Fal1p has 55% identity and 73% similarity on the amino acid level to yeast eIF4A, the prototype of ATP-dependent RNA helicases of the DEAD-box prot...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.17.12.7283
更新日期:1997-12-01 00:00:00
abstract::Gene activation requires alteration of chromatin structure to facilitate active transcription complex formation at a gene promoter. Nucleosome remodeling complexes and histone modifying complexes each play unique and interdependent roles in bringing about these changes. The role of distant enhancers in these structura...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.23.22.8099-8109.2003
更新日期:2003-11-01 00:00:00
abstract::Studies ex vivo have shown that phosphoinositide 3-kinase (PI3K) activity is necessary but not sufficient for insulin-stimulated glucose uptake. Unexpectedly, mice lacking either of the PI3K regulatory subunits p85alpha or p85beta exhibit increased insulin sensitivity. The insulin hypersensitivity is particularly unex...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.25.5.1596-1607.2005
更新日期:2005-03-01 00:00:00
abstract::TBC (Tre-2/Bub2/Cdc16) domains are predicted to encode GTPase-activating proteins (GAPs) for Rab family G proteins. While approximately 50 TBC proteins are predicted to exist in humans, little is known about their substrate specificity. Here we show that TRE17 (also called Tre-2 and USP6), a founding member of the TBC...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.24.22.9752-9762.2004
更新日期:2004-11-01 00:00:00
abstract::Very little is known about the mechanisms mediating longevities of mRNAs. As a means of identifying potential cis- and trans-acting elements which stabilize an individual mRNA, naturally occurring mutations that decreased stability of the normally long-lived globin mRNA were analyzed. Our previous studies demonstrated...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.15.5.2457
更新日期:1995-05-01 00:00:00
abstract::The transcriptional coactivator Cbp plays an important role in a wide range of cellular processes, including proliferation, differentiation, and apoptosis. Although studies have shown its requirement for hematopoietic stem cell (HSC) development, its role in adult HSC maintenance, as well as the cellular and molecular...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.05830-11
更新日期:2011-12-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.7.3929
更新日期:1993-07-01 00:00:00
abstract::Ubiquitously expressed transcription factors play an integral role in establishing and regulating patterns of gene transcription. Common factor 1 (CF1) is a ubiquitously expressed DNA-binding protein previously identified in our laboratory. We show here that CF1 recognizes sites in several diverse transcription elemen...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.11.3.1765
更新日期:1991-03-01 00:00:00
abstract::Protein ubiquitination provides an efficient and reversible mechanism to regulate cell cycle progression and checkpoint control. Numerous regulatory proteins direct the addition of ubiquitin to lysine residues on target proteins, and these are countered by an army of deubiquitinating enzymes (DUBs). BRCA1-associated p...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01517-08
更新日期:2009-04-01 00:00:00