Genotoxic stress prevents Ndd1-dependent transcriptional activation of G2/M-specific genes in Saccharomyces cerevisiae.

abstract：:Endothelial cells differentiate from mesoderm-derived precursors to initiate the earliest events in vascular development. Although the signaling events that regulate the successive steps of vascular development are known in some detail, the transcriptional processes that regulate the first steps in vasculogenesis are ...

journal_title：Molecular and cellular biology

authors： Wu Y,Moser M,Bautch VL,Patterson C

更新日期：2003-08-01 00:00:00

abstract：:The expression of chromosomally integrated transgenes usually varies greatly among independent transfectants. This variability in transgene expression has led to the definition of locus control regions (LCRs) as elements which render expression consistent. Analyses of expression in single cells revealed that the expre...

journal_title：Molecular and cellular biology

authors： Ronai D,Berru M,Shulman MJ

更新日期：1999-10-01 00:00:00

abstract：:The immunoglobulin M heavy-chain locus contains two poly(A) sites which are alternatively expressed during B-cell differentiation. Despite its promoter proximal location, the secretory poly(A) site is not expressed in undifferentiated cells. Crucial to the activation of the secretory poly(A) site during B-cell differe...

journal_title：Molecular and cellular biology

authors： Phillips C,Pachikara N,Gunderson SI

更新日期：2004-07-01 00:00:00

abstract：:To investigate in vivo roles of a murine hypothalamic homeobox gene, Bsx, we generated and analyzed two mutant alleles, Bsx(DeltaHD) and Bsx(lacZ). Bsx(DeltaHD) lacks the homeodomain, and Bsx(lacZ) is an insertion of a lacZ reporter gene. Bsx-lacZ expression was detected in the hypothalamus and pineal gland and reiter...

journal_title：Molecular and cellular biology

authors： McArthur T,Ohtoshi A

更新日期：2007-07-01 00:00:00

abstract：:Budding yeast polo kinase Cdc5p localizes to the spindle pole body (SPB) and to the bud-neck and plays multiple roles during M-phase progression. To dissect localization-specific mitotic functions of Cdc5p, we tethered a localization-defective N-terminal kinase domain of Cdc5p (Cdc5pDeltaC) to the SPB or to the bud-ne...

journal_title：Molecular and cellular biology

authors： Park JE,Park CJ,Sakchaisri K,Karpova T,Asano S,McNally J,Sunwoo Y,Leem SH,Lee KS

更新日期：2004-11-01 00:00:00

abstract：:A single-base deletion within the protein-coding region of the adenovirus type 5 early region 1A (E1A) genes, 399 bases downstream from the transcription start site, depresses transcription to 2% of the wild-type rate. Complementation studies demonstrated that this was due to two effects of the mutation: first, inacti...

journal_title：Molecular and cellular biology

authors： Osborne TF,Arvidson DN,Tyau ES,Dunsworth-Browne M,Berk AJ

更新日期：1984-07-01 00:00:00

abstract：:We have previously shown that activation of extracellular signal-regulated kinase (Erk) by epidermal growth factor (EGF) treatment was significantly decreased in mouse fibroblast cells expressing a mutant Shp-2 molecule lacking 65 amino acids in the SH2-N domain, Shp-2(Delta46-110). To address the molecular mechanism ...

journal_title：Molecular and cellular biology

authors： Shi ZQ,Yu DH,Park M,Marshall M,Feng GS

更新日期：2000-03-01 00:00:00

abstract：:The tumor suppressor gene PTEN is a phosphoinositide phosphatase that is inactivated by deletion and/or mutation in diverse human tumors. Wild-type PTEN is expressed both in the cytoplasm and nucleus in normal cells, with a preferential nuclear localization in differentiated or resting cells. To elucidate the relation...

journal_title：Molecular and cellular biology

authors： Liu JL,Sheng X,Hortobagyi ZK,Mao Z,Gallick GE,Yung WK

更新日期：2005-07-01 00:00:00

abstract：:We report our molecular characterization of the Drosophila melanogaster Abelson gene (abl), a gene in which recessive loss-of-function mutations result in lethality at the pupal stage of development. This essential gene consists of 10 exons extending over 26 kilobase pairs of genomic DNA. The DNA sequence encodes a pr...

journal_title：Molecular and cellular biology

authors： Henkemeyer MJ,Bennett RL,Gertler FB,Hoffmann FM

更新日期：1988-02-01 00:00:00

abstract：:Endonuclease G (endo G) is one of the most abundant nucleases in eukaryotic cells. It is encoded in the nucleus and imported to the mitochondrial intermembrane space. This nuclease is active on single- and double-stranded DNA. We genetically disrupted the endo G gene in mice without disturbing a conserved, overlapping...

journal_title：Molecular and cellular biology

authors： Irvine RA,Adachi N,Shibata DK,Cassell GD,Yu K,Karanjawala ZE,Hsieh CL,Lieber MR

更新日期：2005-01-01 00:00:00

abstract：:The ALCR protein is the transcriptional activator of the ethanol utilization pathway in the filamentous fungus Aspergillus nidulans. This activator belongs to a family of fungal proteins having a conserved DNA-binding domain containing six cysteines (C6 class) with some striking features. At variance with other motifs...

journal_title：Molecular and cellular biology

authors： Kulmburg P,Sequeval D,Lenouvel F,Mathieu M,Felenbok B

更新日期：1992-05-01 00:00:00

abstract：:During mammalian vascular development, endothelial cells form a complex array of vessels that differ markedly in structure and function, but the molecular basis for this vascular complexity is poorly understood. Recent insights into endothelial diversity have come from the identification of molecular markers expressed...

journal_title：Molecular and cellular biology

authors： Xu B,deWaal RM,Mor-Vaknin N,Hibbard C,Markovitz DM,Kahn ML

更新日期：2004-10-01 00:00:00

abstract：:Polycomb group proteins mediate heritable transcriptional silencing and function through multiprotein complexes that methylate and ubiquitinate histones. The 600-kDa E(Z)/ESC complex, also known as Polycomb repressive complex 2 (PRC2), specifically methylates histone H3 lysine 27 (H3 K27) through the intrinsic histone...

journal_title：Molecular and cellular biology

authors： Tie F,Stratton CA,Kurzhals RL,Harte PJ

更新日期：2007-03-01 00:00:00

abstract：:SWI/SNF ATP-dependent chromatin remodeling complexes (remodelers) perform critical functions in eukaryotic gene expression control. BAP and PBAP are the fly representatives of the two evolutionarily conserved major subclasses of SWI/SNF remodelers. Both complexes share seven core subunits, including the Brahma ATPase,...

journal_title：Molecular and cellular biology

authors： Chalkley GE,Moshkin YM,Langenberg K,Bezstarosti K,Blastyak A,Gyurkovics H,Demmers JA,Verrijzer CP

更新日期：2008-05-01 00:00:00

abstract：:The punc gene, encoding a member of the neural cell adhesion molecule family expressed in the developing central nervous system, limbs, and inner ear, was identified. To extend studies of the normal expression pattern of punc and to determine its function, a mouse strain bearing a lacZ/neo insertion in a 5' coding exo...

journal_title：Molecular and cellular biology

authors： Yang W,Li C,Mansour SL

更新日期：2001-09-01 00:00:00

abstract：:T-cell activation requires two different signals. The T-cell receptor's recognition of a specific antigen on antigen-presenting cells provides one, and the second signal comes from costimulatory molecules such as CD28. In contrast, T cells that are stimulated with antigen in the absence of the CD28 costimulatory signa...

journal_title：Molecular and cellular biology

authors： Lai JH,Horvath G,Subleski J,Bruder J,Ghosh P,Tan TH

更新日期：1995-08-01 00:00:00

abstract：:The 3' regulatory region (3' RR) of the murine immunoglobulin heavy chain (IgH) locus contains multiple DNase I-hypersensitive (hs) sites. Proximal sites hs3A, hs1.2, and hs3B are located in an extensive palindromic region and together with hs4 are associated with enhancers involved in the expression and class switch ...

journal_title：Molecular and cellular biology

authors： Giambra V,Volpi S,Emelyanov AV,Pflugh D,Bothwell AL,Norio P,Fan Y,Ju Z,Skoultchi AI,Hardy RR,Frezza D,Birshtein BK

更新日期：2008-10-01 00:00:00

abstract：:We have analyzed the 5'-flanking region of one of the genes coding for the human acute-phase protein, serum amyloid A (SAA). We found that SAA mRNA could be increased fivefold in transfected cells by treatment with phorbol 12-myristate 13-acetate (PMA). To analyze this observation further, we placed a 265-base-pair 5'...

journal_title：Molecular and cellular biology

authors： Edbrooke MR,Burt DW,Cheshire JK,Woo P

更新日期：1989-05-01 00:00:00

abstract：:F8dl is a simian virus 40 early-region deletion mutant that lacks the sequences between 0.169 and 0.423 map units. We show that cloned F8dl DNA immortalized early-passage Fisher rat embryo cells with an efficiency that was about 20% of that of cloned wild-type simian virus 40 DNA. In contrast, we detected no immortali...

journal_title：Molecular and cellular biology

authors： Sompayrac L,Danna KJ

更新日期：1985-05-01 00:00:00

abstract：:A 215-base-pair (bp) region of the mouse MOPC 41 kappa light-chain immunoglobulin gene enhancer has been analyzed for specific binding of lymphoid and nonlymphoid nuclear factors. Mobility shift assays with a series of overlapping DNA fragments have mapped DNA-binding sites for three unique factors. The B-cell-specifi...

journal_title：Molecular and cellular biology

authors： Currie RA,Roeder RG

更新日期：1989-10-01 00:00:00

abstract：:Nuclear migration and positioning within cells are critical for many developmental processes and are governed by the cytoskeletal network. Although mechanisms of nuclear-cytoskeletal attachment are unclear, growing evidence links a novel family of nuclear envelope (NE) proteins that share a conserved C-terminal SUN (S...

journal_title：Molecular and cellular biology

authors： Haque F,Lloyd DJ,Smallwood DT,Dent CL,Shanahan CM,Fry AM,Trembath RC,Shackleton S

更新日期：2006-05-01 00:00:00

abstract：:Checkpoints prevent inaccurate chromosome segregation by inhibiting cell division when errors in mitotic processes are encountered. We used a temperature-sensitive mutation, dbf4, to examine the requirement for DNA replication in establishing mitotic checkpoint arrest. We used gamma-irradiation to induce DNA damage an...

journal_title：Molecular and cellular biology

authors： Tavormina PA,Wang Y,Burke DJ

更新日期：1997-06-01 00:00:00

abstract：:Each of two severely defective mouse kappa-chain genes has acquired a different intracisternal A particle (IAP) element within one of its introns. One IAP element generated 6-base-pair direct repeats upon insertion. In contrast, the other IAP element was not flanked by direct repeats and was missing a single nucleotid...

journal_title：Molecular and cellular biology

authors： Hawley RG,Shulman MJ,Hozumi N

更新日期：1984-12-01 00:00:00

abstract：:Alternative splicing of the locus AbetaH-J-J generates three functionally distinct proteins: an enzyme, AbetaH (aspartyl-beta-hydroxylase), a structural protein of the sarcoplasmic reticulum membrane (junctin), and an integral membrane calcium binding protein (junctate). Junctin and junctate are two important proteins...

journal_title：Molecular and cellular biology

authors： Feriotto G,Finotti A,Volpe P,Treves S,Ferrari S,Angelelli C,Zorzato F,Gambari R

更新日期：2005-04-01 00:00:00

abstract：:The G2-to-M transition (or prophase) checkpoint of the cell cycle is a critical regulator of mitotic entry. SIRT2, a tumor suppressor gene, contributes to the control of this checkpoint by blocking mitotic entry under cellular stress. However, the mechanism underlying both SIRT2 activation and regulation of the G2-to-...

journal_title：Molecular and cellular biology

authors： Movahedi Naini S,Sheridan AM,Force T,Shah JV,Bonventre JV

更新日期：2015-11-01 00:00:00

abstract：:Type I interferons (IFNs) stimulate transcription through a latent heterotrimeric transcription factor composed of tyrosine-phosphorylated STAT1 and STAT2 and the DNA binding partner IRF9, with STAT2 contributing a critical transactivation domain. Human RVB1 and RVB2, which are highly conserved AAA(+) ATP binding prot...

journal_title：Molecular and cellular biology

authors： Gnatovskiy L,Mita P,Levy DE

更新日期：2013-10-01 00:00:00

abstract：:We previously created a knock-in mutant mouse harboring a dominantly negative mutant thyroid hormone receptor beta (TRbeta(PV/PV) mouse) that spontaneously develops a follicular thyroid carcinoma similar to human thyroid cancer. We found that beta-catenin, which plays a critical role in oncogenesis, was highly elevate...

journal_title：Molecular and cellular biology

authors： Guigon CJ,Zhao L,Lu C,Willingham MC,Cheng SY

更新日期：2008-07-01 00:00:00

abstract：:Endonuclease decay of nonsense-containing beta-globin mRNA in erythroid cells generates 5'-truncated products that were reported previously to have a cap or caplike structure. We confirmed that this 5' modification is indistinguishable from the cap on full-length mRNA, and Western blotting, immunoprecipitation, and ac...

journal_title：Molecular and cellular biology

authors： Otsuka Y,Kedersha NL,Schoenberg DR

更新日期：2009-04-01 00:00:00

abstract：:Tumor progression locus 2 (TPL-2) functions as a MEK-1/2 kinase, which is essential for Toll-like receptor 4 (TLR4) activation of extracellular signal-regulated kinase 1 and 2 (ERK-1/2) mitogen-activated protein (MAP) kinases in lipopolysaccharide (LPS)-stimulated macrophages and for inducing the production of the pro...

journal_title：Molecular and cellular biology

authors： Roget K,Ben-Addi A,Mambole-Dema A,Gantke T,Yang HT,Janzen J,Morrice N,Abbott D,Ley SC

更新日期：2012-11-01 00:00:00

abstract：:It is well established that steroid receptor function requires interaction with coactivators. However, the mechanisms through which steroid receptors elicit precise assembly of coactivator complexes and the way the steroid activation signal is transduced remain elusive. Using a T47D cell line stably integrated with a ...

journal_title：Molecular and cellular biology

authors： Li X,Wong J,Tsai SY,Tsai MJ,O'Malley BW

更新日期：2003-06-01 00:00:00