Partial purification and characterization of an ovarian tripeptidyl peptidase: a lysosomal exopeptidase that sequentially releases collagen-related (Gly-Pro-X) triplets.

abstract：:The sphingolipids, sphingosine (SPH), sphingosylphosphorylcholine (SPC) and psycosine induce a rapid and transient rise in nuclear free Ca2+ concentration in a dose dependent manner. To determine whether these sphingolipids act by a IP3-dependent pathway, we tested the increase of Ca2+ in the presence of heparin, an a...

journal_title：Biochemical and biophysical research communications

authors： Catalán RE,Miguel BG,Calcerrada MC,Ruiz S,Martínez AM

更新日期：1997-09-18 00:00:00

abstract：:Lysinuric protein intolerance (LPI; MIM 222700) is an autosomal recessive disorder characterized by defective transport of cationic amino acids lysine, arginine and ornithine. The defect is localized in the basolateral membrane of polar epithelial cells of the renal tubules and intestine. The SLC7A7 (solute carrier fa...

journal_title：Biochemical and biophysical research communications

authors： Toivonen M,Mykkänen J,Aula P,Simell O,Savontaus ML,Huoponen K

更新日期：2002-03-15 00:00:00

abstract：:DNA cleavage by N-(O,O-diisopropyl)phosphoryl histidine(DIPP-His) is investigated by a submarine agarose electrophoresis technique. It was found that DIPP-His could cleave supercoiled DNAs and lambda DNA without the participation of divalent metal ions, while the histidine itself and N-(O,O-diisopropyl)phosphoryl alan...

journal_title：Biochemical and biophysical research communications

authors： Li Y,Sha YW,Ma Y,Zhao Y

更新日期：1995-08-24 00:00:00

abstract：:Under nitrosative stressed condition intracellular GSNO accumulation is common to all cell types. Conserved NADH-dependent GSNO reductase was reported previously as an important cellular protective measure against this. In spite of the constitutive nature of the enzyme, we observed in vivo inactivation of two importan...

journal_title：Biochemical and biophysical research communications

authors： Sahoo R,Sengupta R,Ghosh S

更新日期：2003-03-21 00:00:00

abstract：:alpha-Difluoromethylornithine (DFMO) has been widely used for determining the amounts of ornithine decarboxylase (ODC) in cells. However, we have now found that DFMO does not react with ODC-antizyme complex, indicating that the amounts of radioactive DFMO bound to the protein may not reflect the amounts of ODC in crud...

journal_title：Biochemical and biophysical research communications

authors： Kitani T,Fujisawa H

更新日期：1986-06-30 00:00:00

abstract：:Exosomes are 50-100-nm-diameter membrane vesicles released from various types of cells. Exosomes retain proteins, mRNAs and miRNAs, which can be transported to surrounding cells. CD109 is a glycosylphosphatidylinositol-anchored glycoprotein, and is released from the cell surface to the culture medium in vitro. Recentl...

journal_title：Biochemical and biophysical research communications

authors： Sakakura H,Mii S,Hagiwara S,Kato T,Yamamoto N,Hibi H,Takahashi M,Murakumo Y

更新日期：2016-01-22 00:00:00

abstract：:The tissue targeting and rate of clearance of cobra venom factor (CVF) from the circulation was studied in mice by intravenous or intraperitoneal injection of radioiodinated CVF. In both modes of administrations, CVF was targeted mainly to liver. CVF injected directly into the blood was cleared from the circulation wi...

journal_title：Biochemical and biophysical research communications

authors： Fu Q,Satyaswaroop PG,Gowda DC

更新日期：1997-02-13 00:00:00

abstract：:Primary rat hippocampal neurones show pronounced elevations of intracellular calcium within minutes of exposure to the HIV coat protein gp120. Culture of hippocampal neurones with gp120 causes significant neurotoxicity. We find that the peptide VSLSYRCPCRFF, a competitive inhibitor of the CXCR4 chemokine receptor, mar...

journal_title：Biochemical and biophysical research communications

authors： Pandey V,Bolsover SR

更新日期：2000-07-21 00:00:00

abstract：:The aim of the present study was to examine the effects of p-coumaric acid on the longitudinal growth of the long bone in adolescent male rats. Teatment with p-coumaric acid significantly increased the tibial length and the height of each growth plate zone and the ratio of 5-bromo-2'-deoxyuridine-positive cells relati...

journal_title：Biochemical and biophysical research communications

authors： Lee JH,Chung YH,Kim HH,Bang JS,Jung TW,Park T,Park J,Kim U,Lee SH,Jeong JH

更新日期：2018-11-10 00:00:00

abstract：:Galectin-9 (G9) is a tandem-repeat type β-galactoside-specific animal lectin having N-terminal and C-terminal carbohydrate recognition domains (N-CRD and C-CRD, respectively) joined by a linker peptide that is involved in the immune system. G9 is divalent in glycan binding, and structural information about the spatial...

journal_title：Biochemical and biophysical research communications

authors： Yoshida H,Nishi N,Wada K,Nakamura T,Hirashima M,Kuwabara N,Kato R,Kamitori S

更新日期：2017-09-02 00:00:00

abstract：:Drosophila Dicer-2 processes RNA substrates into short interfering RNAs (siRNAs). Loquacious-PD (Loqs-PD), a dsRNA-binding protein that associates with Dicer-2, is required for processing of a subset of RNA substrates including hairpin RNAs into siRNAs. Inorganic phosphate-a small molecule present in all cell types-in...

journal_title：Biochemical and biophysical research communications

authors： Fukunaga R

更新日期：2018-04-15 00:00:00

abstract：:Antibody-based targeted therapy of cancers requires the antibody targeting of specific molecules inducing tumor cells apoptosis or death. Angiopoietin-2 (Agn-2) and translocator protein (TSPO) are identified as potential target molecules for glioblastoma therapy. The single chain anti-Agn-2 antibody (Anag-2) and anti-...

journal_title：Biochemical and biophysical research communications

authors： Li J,Zhang Z,Lv L,Qiao H,Chen X,Zou C

更新日期：2016-04-01 00:00:00

abstract：:PI3K activation is commonly observed in many human cancer cells. Survivin expression is elevated in cancer cells, and induced by some growth factors through PI3K activation. However, it is not clear whether PI3K activation is sufficient to induce survivin expression. To investigate the role of PI3K pathway in the regu...

journal_title：Biochemical and biophysical research communications

authors： Zhao P,Meng Q,Liu LZ,You YP,Liu N,Jiang BH

更新日期：2010-04-30 00:00:00

abstract：:Apolipoprotein (apo-) B mRNA editing at nucleotide 6666 converts cytidine to uridine, transforming the codon for glutamine-2153 to a termination codon. To investigate this editing mechanism, [a-32P] and [5-3H] CTP were incorporated into synthetic apo-B RNA. After the substrate had been edited extensively in vitro by a...

journal_title：Biochemical and biophysical research communications

authors： Johnson DF,Poksay KS,Innerarity TL

更新日期：1993-09-30 00:00:00

abstract：:The Z-DNA binding domain of human ADAR1 (Zα(ADAR1)) preferentially binds Z-DNA rather than B-DNA with high binding affinity. Here, we have carried out chemical shift perturbation and backbone dynamics studies of Zα(ADAR1) in the free form and in complex with three DNA duplexes, d(CGCGCG)(2), d(CACGTG)(2), and d(CGTACG...

journal_title：Biochemical and biophysical research communications

authors： Lee AR,Kim HE,Lee YM,Jeong M,Choi KH,Park JW,Choi YG,Ahn HC,Choi BS,Lee JH

更新日期：2012-11-09 00:00:00

abstract：:The microvesicles (MVs) in bovine posterior pituitaries contain the reserpine-sensitive vesicular monoamine transporter (Moriyama et al. (1995) J. Biol. Chem. 270, 11424-11429). An antibody against the N-terminal region of the monoamine transporter from bovine chromaffin granules recognized a polypeptide in the MVs wi...

journal_title：Biochemical and biophysical research communications

authors： Moriyama Y,Yamada H

更新日期：1996-04-25 00:00:00

abstract：:Wortmannin caused normal and strong inhibition on catecholamine secretion from bovine adrenal chromaffin cells and in vitro phosphoinositide 3-kinase activity in NaCl-, Na isethionate-, choline Cl-, Na acetate-, and N-acetyl glycine-based media. However, brief preincubation of wortmannin with the media containing amin...

journal_title：Biochemical and biophysical research communications

authors： Isosaki M

更新日期：2004-11-26 00:00:00

abstract：:Transthyretin (TTR) is a human disease-associated amyloidogenic protein that has been implicated in senile systemic amyloidosis (SSA) and familial amyloidotic polyneuropathy (FAP). FAP typically results in severe and early-onset disease, and the only therapy established so far is liver transplantation; thus, developin...

journal_title：Biochemical and biophysical research communications

authors： Lee KW,Lee DH,Son H,Kim YS,Park JY,Roh GS,Kim HJ,Kang SS,Cho GJ,Choi WS

更新日期：2009-10-16 00:00:00

abstract：:Changes in phospholipid and fatty acid profile are hallmarks of cancer progression. Increase in peripheral benzodiazepine receptor expression has been implicated in breast cancer. The benzodiazepine, Ro5-4864, increases cell proliferation in some breast cancer cell lines. Biosynthesis of phosphatidylcholine (PC) has b...

journal_title：Biochemical and biophysical research communications

authors： Akech J,Sinha Roy S,Das SK

更新日期：2005-07-22 00:00:00

abstract：:Large tonsillar lymphocytes labeled with [3H]thymidine reverted to small lymphocytes with concomitant loss of [3H]DNA upon culturing. The decrease of labeled DNA content and size of large lymphocytes was demonstrated by flow cytometry and cell sorting. These observations suggest that stimulated lymphocytes may revert ...

journal_title：Biochemical and biophysical research communications

authors： Sasvari-Szekely M,Staub M,Taljanidisz J,Szabo G Jr,Takacs J,Antoni F

更新日期：1984-12-28 00:00:00

abstract：:It has long been assumed that Ca2+ are translocated from the cytosol to the cell nucleus by a long distance to activate transcription machinery buried deep in the nucleoplasm. However, this model has been recently challenged. When HeLa cells were loaded with fluo-3, highly fluorescent spots of approximately 2 microns ...

journal_title：Biochemical and biophysical research communications

authors： Lui PP,Kong SK,Kwok TT,Lee CY

更新日期：1998-06-09 00:00:00

abstract：:Cytoskeletal reorganization processes can be analyzed by studying the nanometer-scale spontaneous motion of beads bound to the cytoskeleton. The bead motion is determined by force fluctuations within the cytoskeletal network that originates from myosin motor activity and dynamic restructuring of cytoskeletal filaments...

journal_title：Biochemical and biophysical research communications

authors： Alonso JL,Goldmann WH

更新日期：2012-05-04 00:00:00

abstract：:Phage T7 DNA polymerase purified to homogeneity by an antithioredoxin immunoadsorbent technique was resolved into its active subunits the gene 5 protein and Escherichia coli thioredoxin by a novel technique involving chromatography on Sephadex G-50 at pH 11.5. Analysis of the metal content of the holoenzyme by atomic ...

journal_title：Biochemical and biophysical research communications

authors： Slaby I,Lind B,Holmgren A

更新日期：1984-08-16 00:00:00

abstract：:Estrogen receptor (ER) is regarded as a major causal factor in breast cancer and FoxA1, a winged-helix transcription factor belonging to the forkhead family, has been found to function as a pioneer factor in the recruitment of ER to several cis-regulatory elements in the genome. High throughput chromatin immunoprecipi...

journal_title：Biochemical and biophysical research communications

authors： Liu Z,Chen S

更新日期：2010-09-10 00:00:00

abstract：:Bovine cytochrome c oxidase in 0.1% dodecylmaltoside, 50 mM KCl and 10 mM Tris-HCl, pH 7.4 is monodisperse with an apparent Mr 360,000 (dimer) as estimated by filtration on Ultrogel AcA 34. In the absence of added KCl the apparent Mr is 160,000 (monomer). The dimeric enzyme has a high and a low affinity site for cytoc...

journal_title：Biochemical and biophysical research communications

authors： Nałecz KA,Bolli R,Azzi A

更新日期：1983-07-29 00:00:00

abstract：:We have developed a continuous fluorescence assay based on fluorescence resonance energy transfer (FRET) for the monitoring of RNA helicase activity in vitro. The assay is tested using the hepatitis C virus (HCV) NS3 helicase as a model. We prepared a double-stranded RNA (dsRNA) substrate with a 5' fluorophore-labeled...

journal_title：Biochemical and biophysical research communications

authors： Tani H,Fujita O,Furuta A,Matsuda Y,Miyata R,Akimitsu N,Tanaka J,Tsuneda S,Sekiguchi Y,Noda N

更新日期：2010-02-26 00:00:00

abstract：:Rat liver ornithine decarboxylase induction by dexamethasone or laparatomy, which is dramatically impaired by catecholamine depletion, is not affected by alpha-and beta -adrenergic blockers administered simultaneously 1 h prior to steroid injection or operation. However, if blockade is maintained for 24 h, an effect c...

journal_title：Biochemical and biophysical research communications

authors： Astancolle S,Davalli P,Corti A

更新日期：1991-01-31 00:00:00

abstract：:Elafin, a human skin derived inhibitor of human leukocyte elastase, was tested for inhibitory activity against proteinase 3, an elastin degrading proteinase of neutrophils. The inhibitory activity of elafin was compared with antileukoprotease and eglin C. Elafin proved to be a potent inhibitor of elastin-FITC degradat...

journal_title：Biochemical and biophysical research communications

authors： Wiedow O,Lüademann J,Utecht B

更新日期：1991-01-15 00:00:00

abstract：:Signaling by stem cell factor and Kit, its receptor, play important roles in gametogenesis, hematopoiesis, mast cell development and function, and melanogenesis. Moreover, human and mouse embryonic stem cells express Kit transcripts. Stem cell factor exists as both a soluble and a membrane-bound glycoprotein while Kit...

journal_title：Biochemical and biophysical research communications

authors： Roskoski R Jr

更新日期：2005-12-23 00:00:00

abstract：:3q21q26 syndrome, an acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) with chromosomal translocations or inversions between the bands 3q21 and 3q26, is frequently associated with dysmegakaryocytopoiesis and increased platelet counts at the initial diagnosis. Since the EVI1 gene at 3q26 is transcriptional...

journal_title：Biochemical and biophysical research communications

authors： Shimizu S,Nagasawa T,Katoh O,Komatsu N,Yokota J,Morishita K

更新日期：2002-04-05 00:00:00