Regulation of lipoprotein lipase secretion by mouse peritoneal macrophages.

abstract：:The presence of a specific receptor for 1,25-dihydroxyvitamin D (1,25(OH)2D) was investigated in a cell line A7r5 derived from fetal aorta. 1,25(OH)2[3H]D3 binding to cytosol was saturated at 0.6-1 nM, and Scatchard analysis yielded dissociation constant and binding sites, (3.02 +/- 0.4) X 10(-11) M and 33.9 +/- 5.8 f...

journal_title：Biochemical and biophysical research communications

authors： Kawashima H

更新日期：1987-07-15 00:00:00

abstract：:To develop antibodies against the plasma membrane 3,3',5-triiodo-L-thyronine (T3) binding protein (M.W. 55,000), rabbits were immunized with formalin-fixed GH3 cells or highly purified plasma membranes from these cells. Antibodies were screened by immunoprecipitation using detergent solubilized N-bromoacetyl-[125I]T3-...

journal_title：Biochemical and biophysical research communications

authors： Hasumura S,Rossi B,Alderson R,Pastan I,Cheng S

更新日期：1984-11-14 00:00:00

abstract：:We have isolated a cDNA encoding human epidermal transglutaminase, a key enzyme of terminal differentiation of keratinocytes. A cDNA library from cultured human keratinocytes was screened by a PCR-amplified partial cDNA fragment of the enzyme with oligonucleotide primers based on the homology of the transglutaminase f...

journal_title：Biochemical and biophysical research communications

authors： Yamanishi K,Liew FM,Konishi K,Yasuno H,Doi H,Hirano J,Fukushima S

更新日期：1991-03-29 00:00:00

abstract：:Constitutive and PKC-regulated alpha-secretase pathways have been reported to produce the secreted form of alpha-secretase-derived APP (sAPPalpha). Here, we examined putative role of furin in the regulation of alpha-secretase activity in vitro and in vivo. Overexpression of the prodomain of furin and infection with a ...

journal_title：Biochemical and biophysical research communications

authors： Hwang EM,Kim SK,Sohn JH,Lee JY,Kim Y,Kim YS,Mook-Jung I

更新日期：2006-10-20 00:00:00

abstract：:Kinetic studies of the reduction of Pseudomonas aeruginosa ferricytochrome c551 by Fe(EDTA)2- have been made. The reaction was found to follow a second-order rate law: k 4.2 x 10(3) M(-1) s(-1) [25 degrees, micro0.1 M, pH 7.0 (phosphate)]; deltaH+/+ 3.2 kcal/ mol; AS+/+ -30 cal/mol-deg. The electrostatics-corrected se...

journal_title：Biochemical and biophysical research communications

authors： Coyle CL,Gray HB

更新日期：1976-12-20 00:00:00

abstract：:Glycoprotein130 (gp130) is an important signal transducer in the interleukin-6 (IL-6) trans-signaling pathway, which plays a crucial role in chronic inflammation in atherosclerosis. Studies suggest that estrogen can inhibit IL-6/gp130 signaling and reduce the risk of coronary artery disease, but the precise mechanism ...

journal_title：Biochemical and biophysical research communications

authors： Zhou M,Dai W,Cui Y,Li Y

更新日期：2020-03-12 00:00:00

abstract：:The susceptibility to hydrolysis of LHRH and the decapeptide analogue Antide has been compared. The hydrolysis of LHRH by pig kidney brush border membranes is inhibited by phosphoramidon (I50 = 5.6 nM) implicating endopeptidase-24.11 in the initiation of hydrolysis. Under conditions in which LHRH is fully degraded by ...

journal_title：Biochemical and biophysical research communications

authors： Medeiros Mdos S,Turner AJ,Ljungqvist A,Folkers K

更新日期：1991-04-15 00:00:00

abstract：:The polycationic nature of biogenic polyamines such as spermine (SPM, 1) and spermidine (SPD, 2) plays an important role in selective binding to polyanionic nucleic acids. These interactions are mediated by electrostatic and hydrogen bonding forces which led to stabilization of DNA duplexes and triplexes. Transformati...

journal_title：Biochemical and biophysical research communications

authors： Pallan PS,Ganesh KN

更新日期：1996-05-15 00:00:00

abstract：:Eicosapentaenoic acid (EPA; 20:5 n-3; ethyl ester) in combination with atherogenic or non-atherogenic high fat diets was fed to young adult male marmoset monkeys for a period of 30 weeks. EPA markedly reduced the raised plasma cholesterol level evident when feeding an atherogenic diet but did not influence the cardiac...

journal_title：Biochemical and biophysical research communications

authors： Patten GS,Rinaldi JA,McMurchie EJ

更新日期：1989-07-31 00:00:00

abstract：:Relative orientation of recombination substrates, neo gene, strongly influenced homologous recombination events in a bovine papillomavirus shuttle vector. Between direct repeats, recombination occurred at a high frequency while between inverted repeats, it was rare. Double strand break near the mutation site increased...

journal_title：Biochemical and biophysical research communications

authors： Naito A,Kitamura Y,Yoshikura H

更新日期：1991-01-15 00:00:00

abstract：:Angiotensin II (Ang II) modulates VSMCs phenotypic switch that play a critical role in the cardiovascular diseases. MicroRNA-27a (miR-27a) has been proven to be involved in regulating vascular remodeling; however, the functional role of miR-27a in VSMCs in response to Ang II stimulation need to be elucidated. Cell pro...

journal_title：Biochemical and biophysical research communications

authors： Xu MM,Deng HY,Li HH

更新日期：2019-02-19 00:00:00

abstract：:The Saccharomyces cerevisiae gene VPS35 encodes a component of the retromer complex which is involved in vesicle transport from endosomes to the trans-Golgi network. Yeast and human VPS35 orthologs are highly conserved and mutations in human VPS35 cause an autosomal dominant form of late-onset Parkinson disease (PD). ...

journal_title：Biochemical and biophysical research communications

authors： Sowada N,Stiller B,Kubisch C

更新日期：2016-08-05 00:00:00

abstract：:The first several months of life are a critical period for neuronal plasticity in the visual cortex, during which anatomical and physiological development depends upon visual experience. Rearing in darkness slows the time course of this critical period, such that at 5 weeks normal cats are more plastic than dark-reare...

journal_title：Biochemical and biophysical research communications

authors： Yang C,Silver B,Ellis SR,Mower GD

更新日期：2001-10-12 00:00:00

abstract：:Isocitrate dehydrogenase 1 (IDH1) mutations have been detected in gliomas and other tumors. Although IDH1 catalyzes the oxidative carboxylation of isocitrate to α-ketoglutarate (α-KG) in cytosol, mutated IDH1 proteins possess the ability to change α-KG into the oncometabolite D-2-hydroxyglutarate (D-2HG). Several mono...

journal_title：Biochemical and biophysical research communications

authors： Fujii Y,Ogasawara S,Oki H,Liu X,Kaneko MK,Takano S,Kato Y

更新日期：2015-10-30 00:00:00

abstract：:Oscillatory gene expression plays an important role in somite segmentation during the early developmental stages of vertebrates. Recent experimental studies have shown that microRNA can regulate gene expression by stimulating degradation of mRNA and/or repression of translation. In this communication, we incorporate m...

journal_title：Biochemical and biophysical research communications

authors： Xie ZR,Yang HT,Liu WC,Hwang MJ

更新日期：2007-07-06 00:00:00

abstract：:Viral vectors displaying specific ligand binding moieties have raised an increasing interest in the area of targeted gene therapy. In this report, we describe baculovirus vectors displaying either a functional single chain antibody fragment (scFv) specific for the carcinoembryonic antigen (CEA) or the synthetic IgG bi...

journal_title：Biochemical and biophysical research communications

authors： Ojala K,Mottershead DG,Suokko A,Oker-Blom C

更新日期：2001-06-15 00:00:00

abstract：:The mRNA of the mitochondrial uncoupling protein 2 (UCP2) was up-regulated by cryptorchidism, a testicular hyperthermic condition under which germ cells undergo severe apoptosis. We investigated whether UCP2 was able to protect germ cells from hyperthermia-induced apoptosis. UCP2 was predominantly present in elongate ...

journal_title：Biochemical and biophysical research communications

authors： Zhang K,Shang Y,Liao S,Zhang W,Nian H,Liu Y,Chen Q,Han C

更新日期：2007-08-24 00:00:00

abstract：:DNA bending has been shown to play a critical role in conservative site-specific DNA recombination reactions such as lambda integration. V(D)J recombination, the only mammalian site directed recombination system, is directed by recombinational signal sequences composed of heptamer, nonamer and spacer elements. The non...

journal_title：Biochemical and biophysical research communications

authors： Andrews R,Halligan NL,Halligan BD

更新日期：1993-05-28 00:00:00

abstract：:Mutations in protein kinase Cgamma (PKCgamma) cause the neurodegenerative disease spinocerebellar ataxia type 14 (SCA14). In this study, expression of an extensive panel of known SCA14-associated PKCgamma mutations as fusion proteins in cell culture led to the consistent formation of cytoplasmic aggregates in response...

journal_title：Biochemical and biophysical research communications

authors： Doran G,Davies KE,Talbot K

更新日期：2008-08-01 00:00:00

abstract：:The effect of secretory phospholipase A2 (sPLA2) on intracellular Ca2+ signaling in human astrocytoma cells was studied. sPLA2 increased cytosolic [Ca2+] ([Ca2+]c) in both Ca2+-containing and Ca2+-free medium, thus suggesting Ca2+ release from intracellular stores. The activation by sPLA2 of arachidonate release via c...

journal_title：Biochemical and biophysical research communications

authors： Hernández M,Barrero MJ,Alvarez J,Montero M,Sánchez Crespo M,Nieto ML

更新日期：1999-06-24 00:00:00

abstract：:Nonalcoholic fatty liver disease (NAFLD) is featured by hepatic steatosis, insulin resistance, lipid deposition and inflammation. However, the pathogenic mechanism of NAFLD is still poorly understood. Dual-specificity phosphatase 16 (DUSP16), a c-Jun N-terminal kinase-specific phosphatase, has been reported to negativ...

journal_title：Biochemical and biophysical research communications

authors： Wu YK,Hu LF,Lou DS,Wang BC,Tan J

更新日期：2020-03-26 00:00:00

abstract：:Plasminogen activation by tissue-plasminogen activator (t-PA) is accelerated by the presence of a macromolecular surface, which acts as a template that brings enzyme and substrate in close proximity. Modification of lysine residues, which are important for this template function, occurs in diabetic patients as a conse...

journal_title：Biochemical and biophysical research communications

authors： Bobbink IW,Tekelenburg WL,Sixma JJ,de Boer HC,Banga JD,de Groot PG

更新日期：1997-11-26 00:00:00

abstract：:Aminoglycosides are important antibiotics used against a wide range of pathogens. As a mechanism of defense, bacteria have evolved enzymes able to inactivate these drugs by regio-selectively adding a variety of functionalities (acetyl, phospho, and nucelotidyl groups) to their scaffolds. The aminoglycoside nucleotidyl...

journal_title：Biochemical and biophysical research communications

authors： Porter VR,Green KD,Zolova OE,Houghton JL,Garneau-Tsodikova S

更新日期：2010-12-03 00:00:00

abstract：:SMCX/JARID1C was recently shown to be a histone H3 lysine 4 (H3K4) demethylase. Here, we have identified an SMCX isoform that predominantly resides in the cytoplasm, but still efficiently demethylates trimethylated H3K4. SMCX requires several functional domains for its demethylase activity and is also capable of formi...

journal_title：Biochemical and biophysical research communications

authors： Kim TD,Shin S,Janknecht R

更新日期：2008-02-08 00:00:00

abstract：:We report here on the cloning and functional characterization of human LAT1, a subunit of the amino acid transport system L. The hLAT1 cDNA, obtained from a human placental cDNA library, codes for a protein of 507 amino acids. When functionally expressed in mammalian cells together with the heavy chain of the rat 4F2 ...

journal_title：Biochemical and biophysical research communications

authors： Prasad PD,Wang H,Huang W,Kekuda R,Rajan DP,Leibach FH,Ganapathy V

更新日期：1999-02-16 00:00:00

abstract：:Peptides with a molecular weight of 5 kDa have been isolated from seeds of the pinto bean and red bean, respectively. The peptides manifest an N-terminal sequence with remarkable resemblance to those of cowpea 10-kDa protein precursor and garden pea disease resistance response protein. The bean peptides possess potent...

journal_title：Biochemical and biophysical research communications

authors： Ye XY,Ng TB

更新日期：2001-07-13 00:00:00

abstract：:We provide the first demonstration for a Na+/Ca2+ exchanger, NCX-1, in the osteoclast. We speculate that by using Na+ exchange, NCX-1 couples H+ extrusion with Ca2+ fluxes during bone resorption. Microspectrofluorimetry of fura-2-loaded osteoclasts revealed a rapid and sustained, but reversible, cytosolic Ca2+ elevati...

journal_title：Biochemical and biophysical research communications

authors： Moonga BS,Davidson R,Sun L,Adebanjo OA,Moser J,Abedin M,Zaidi N,Huang CL,Zaidi M

更新日期：2001-05-18 00:00:00

abstract：:Using baculoviral and bacterial systems, we expressed biologically active recombinant rat IL-13 and generated neutralizing rat IL-13 antiserum. Recombinant rat IL-13 produced by baculovirus-infected insect cells stimulated proliferation of TF-1 premyeloid cell line and induced expression of 15-lipoxygenase mRNA in hum...

journal_title：Biochemical and biophysical research communications

authors： Lakkis FG,Cruet EN,Nassar GM,Badr KF,Pascual DW

更新日期：1997-06-27 00:00:00

abstract：:The purpose of the present study was to investigate the effect of acute exercise on lipolysis via coordination of hormone-sensitive lipase (HSL) and scaffold proteins, i.e., perilipin A and comparative gene identification-58 (CGI-58), in rat primary adipocytes. Glycerol release was significantly elevated immediately (...

journal_title：Biochemical and biophysical research communications

authors： Ogasawara J,Nomura S,Rahman N,Sakurai T,Kizaki T,Izawa T,Ishida H,Haga S,Ohno H

更新日期：2010-09-10 00:00:00

abstract：:A subclone termed as HSG-S8 was previously isolated from human salivary adenocarcinoma cell line, HSG, and proliferated in a serum-free culture. When HSG-S8 and parental HSG cells were transplanted into nude mice i.m. or s.c., both cells reproducibly induced adenocarcinoma. Furthermore, injection of HSG-S8 consistentl...

journal_title：Biochemical and biophysical research communications

authors： Hatakeyama S,Ohara-Nemoto Y,Kyakumoto S,Satoh M

更新日期：1993-02-15 00:00:00