Abstract：
:Polyspermic Xenopus laevis eggs can be identified easily because of regions of pigment accumulation and white stripes, which arise by a nocodazole-sensitive process. Eggs containing up to four sperm are capable of forming a single embryonic axis. Dispermic eggs display two regions of pigment accumulation, one around each sperm entry point (SEP), and one white stripe between the SEPs. Such eggs with a 180 degree separation between the SEPs were bisected before first cleavage along the white stripe, creating dorsal and ventral halves in many cases. Each half cleaved and formed a tadpole. When eggs were bisected early in the period of cytoplasmic reorganization (0.5-0.6 normalized time), each half could form a complete tadpole. When eggs were bisected after the period of reorganization (0.8-0.9), often one half formed a tadpole with a complete head but reduced or absent tail and the other half formed a tadpole with a complete tail but reduced or absent head. These results demonstrate that sperm cooperate to give a single embryonic axis in polyspermic eggs and the development of dorsal and ventral egg halves differs after egg reorganization before first cleavage.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Render JA,Elinson RPdoi
10.1016/0012-1606(86)90262-9subject
Has Abstractpub_date
1986-06-01 00:00:00pages
425-33issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(86)90262-9journal_volume
115pub_type
杂志文章abstract：:Aurora A is a mitotic kinase essential for cell proliferation. In mice, ablation of Aurora A results in mitotic arrest and pre-implantation lethality, preventing studies at later stages of development. Here we report the effects of Aurora A ablation on embryo patterning at early post-implantation stages. Inactivation ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.08.010
更新日期：2012-11-01 00:00:00
abstract：:An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.03.011
更新日期：2011-06-01 00:00:00
abstract：:The remarkable regenerative capacity displayed by plants and various vertebrates, such as amphibians, is largely based on the capability of somatic cells to undergo dedifferentiation. In this process, mature cells reverse their state of differentiation and acquire pluripotentiality--a process preceding not only reentr...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2003.12.027
更新日期：2004-04-01 00:00:00
abstract：:The notochord is the defining characteristic of the chordate embryo and plays critical roles as a signaling center and as the primitive skeleton. In this study we show that early notochord development in Xenopus embryos is regulated by apoptosis. We find apoptotic cells in the notochord beginning at the neural groove ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.08.047
更新日期：2007-11-15 00:00:00
abstract：:Fertilization-related sperm mitochondrial movement occurs at a rate comparable to other actin-myosin-driven movements and is inhibited by cytochalasin B and N-ethyl maleimide in Ascidia ceratodes sperm. F-actin was demonstrated in the tails and mitochondria using NBD-phallacidin fluorescence. Both actin and myosin wer...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90229-x
更新日期：1984-12-01 00:00:00
abstract：:The organization of the embryonic neural plate requires coordination of multiple signal transduction pathways, including fibroblast growth factors (FGFs), bone morphogenetic proteins (BMPs), and WNTs. Many studies have suggested that a critical component of this process is the patterning of posterior neural tissues by...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.06.019
更新日期：2009-09-01 00:00:00
abstract：:Oocyte maturation in mouse is associated with a dramatic reorganisation of the endoplasmic reticulum (ER) from a network of cytoplasmic accumulations in the germinal vesicle-stage oocyte (GV) to a network of distinctive cortical clusters in the metaphase II egg (MII). Multiple lines of evidence suggest that this redis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.006
更新日期：2007-05-01 00:00:00
abstract：:Octopamine is an important neuroactive substance that modulates several physiological functions and behaviors of invertebrate species. Its biosynthesis involves two steps, one of which is catalyzed by Tyramine beta-hydroxylase enzyme (TBH). The Tbetah gene has been previously cloned from Drosophila melanogaster, and n...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.07.019
更新日期：2003-12-01 00:00:00
abstract：:The pdx1 gene is essential for pancreatic organogenesis in humans and mice; pdx1 mutations have been identified in human diabetic patients. Specific inactivation of pdx1 in adult beta cells revealed that this gene is required for maintenance of mature beta cell function. In the following study, a Cre-lox strategy was ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.038
更新日期：2008-02-15 00:00:00
abstract：:Developmentally regulated gene expression has been analyzed in the wild-type D. discoideum strain NC-4 and a series of temporally deranged mutants. The mutants include representatives from each class of rapid development mutation, Fr17(rdeA-) and HT506(rdeC-), and strain HIfm-1, which appears to be defective in the ti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90260-5
更新日期：1986-06-01 00:00:00
abstract：:Inhibitor of differentiation and DNA-binding (Id) proteins, Id1 to Id4, function in the regulation of cellular proliferation and differentiation. Id proteins have been shown to interact with bHLH proteins and other proteins involved in regulating cellular proliferation and differentiation, suggesting a widespread regu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.12.006
更新日期：2020-04-15 00:00:00
abstract：:During the process of gastrulation in the sea urchin embryo, the vegetal plate invaginates to form the archenteron, a long narrow tube that extends across the blastocoel. Rearrangement of cells within the archenteron is thought to be a key component of the process of archenteron elongation. While these cell rearrangem...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1154
更新日期：1994-06-01 00:00:00
abstract：:The spermatid nucleus and cytoplasm undergo dramatic morphological modifications during spermatid differentiation into mature sperm. Some of the external force causing this nuclear shaping is generated by a microtubular structure termed the manchette, which attaches to the perinuclear ring of the spermatid. Here, we r...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0286
更新日期：2001-08-15 00:00:00
abstract：:Hippo (Hpo) signaling plays a critical role in restricting tissue growth and organ size in both invertebrate and vertebrate animals. However, how the Hpo kinase is regulated during development has not been clearly understood. Using a Bimolecular Fluorescence Complementation assay, we have investigated the functional s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.12.017
更新日期：2013-03-15 00:00:00
abstract：:Recently, it has been suggested that a mouse sperm-associated protein with properties similar to the inhibitory guanine nucleotide-binding regulatory protein Gi plays an intermediary role in the zona pellucida-induced acrosome reaction (Endo, Y., Lee, M. A., and Kopf, G. S. 1987. Dev. Biol. 119, 210-216). In the prese...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90157-1
更新日期：1988-09-01 00:00:00
abstract：:The changes in the intracellular pH (pHi) of sea urchin sperm associated with motility initiation and acrosome reaction were investigated using uptake of two different probes; 9-aminoacridine and methylamine, as a qualitative index. Sperm suspended in Na+-free sea water were immotile and able to concentrate these amin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90004-0
更新日期：1983-01-01 00:00:00
abstract：:In studies initially focused on roles of nonmuscle myosin IIA (NMIIA) in the developing mouse epidermis, we have discovered that a previously described cytokeratin 5 (K5)-Cre gene construct is expressed in early embryo development. Mice carrying floxed alleles of the nonmuscle myosin II heavy chain gene (NMHC IIA(flox...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.07.017
更新日期：2013-10-01 00:00:00
abstract：:Trypsin-like activity is secreted from eggs of many species at fertilization, and this activity is believed to be critical for the block to polyspermy. Here we show that a cortical granule serine protease of sea urchins is the major and perhaps only protease family member important for fertilization. Zymography assays...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9299
更新日期：1999-07-01 00:00:00
abstract：:During development, cells craft an impressive array of actin-based structures, mediating events as diverse as cytokinesis, apical constriction, and cell migration. One challenge is to determine how cells regulate actin assembly and disassembly to carry out these cell behaviors. During Drosophila oogenesis diverse cell...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.06.030
更新日期：2009-09-01 00:00:00
abstract：:The Drosophila boule gene is expressed exclusively in the male germline and encodes an RNA binding protein closely related to the mammalian fertility factors encoded by the DAZ (Deleted in Azoospermia) and DAZL (DAZ-like) genes. Mutation of boule blocks both meiotic divisions. Differentiation nonetheless continues, re...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9098
更新日期：1998-12-15 00:00:00
abstract：:Most, if not all, endocrine cells seem capable of synthesizing and storing more than one hormone. Such cellular colocalization of hormones can be due either to the presence of two or more specific granules within the cells or to colocalization of the hormones within a single granule. The present study was performed to...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90122-w
更新日期：1992-10-01 00:00:00
abstract：:Oocyte maturation (meiosis reinitiation) in starfish is induced by the natural hormone 1-methyladenine (1-MeAde). Oocytes of Evasterias troschelii contain 0.43 pmole cyclic AMP/mg protein and 0.47 pmole cyclic GMP/mg protein. Upon stimulation by 1-MeAde the oocytes undergo a moderate (10-30%) decrease in their cAMP co...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90166-7
更新日期：1987-06-01 00:00:00
abstract：:Suppression of wing fate and specification of haltere fate in Drosophila by the homeotic gene Ultrabithorax is a classical example of Hox regulation of serial homology (Lewis, E.B. 1978. Nature 276, 565-570) and has served as a paradigm for understanding homeotic gene function. We have used DNA microarray analyses to ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.12.022
更新日期：2006-03-15 00:00:00
abstract：:During chick limb development the gap junction protein Connexin-43 (Cx43) is expressed in discrete spatially restricted domains in the apical ectodermal ridge (AER) and mesenchyme of the zone of polarising activity. Antisense oligonucleotides (ODNs) were used to investigate the role of Connexin-43 (Cx43) in the develo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9171
更新日期：1999-03-15 00:00:00
abstract：:We have identified a cellular efflux pump, RhT, with the properties of an MDR transporter-a type of ATP-binding cassette transporter whose substrates include small hydrophobic molecules. RhT transports rhodamine 123 (Rh123) and is inhibited by low temperature, energy poisons, and several MDR transport inhibitors, such...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9611
更新日期：2000-04-01 00:00:00
abstract：:mid and H15 encode Tbx20 transcription factors that specify ventral pattern in the Drosophila leg. We find that there are at least two pathways for mid and H15 specification of ventral fate. In the first pathway, mid and H15 negatively regulate Dpp, the dorsal signal in leg development. mid and H15 block the dorsalizi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.05.012
更新日期：2019-11-01 00:00:00
abstract：:Fetal human hepatocytes were isolated by collagenase digestion of liver fragments and cultured either alone or mixed with rat liver epithelial cells. Whereas they did not survive more than 2-3 weeks and showed rapid morphologic and functional alterations in conventional culture, fetal hepatocytes survived and retained...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90276-8
更新日期：1984-09-01 00:00:00
abstract：:Cis-regulatory modules (CRMs) control spatiotemporal gene expression patterns in embryos. While measurement of quantitative CRM activities has become efficient, measurement of spatial CRM activities still relies on slow, one-by-one methods. To overcome this bottleneck, we have developed a high-throughput method that c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.01.010
更新日期：2017-02-15 00:00:00
abstract：:We have examined the interfaces between protein and lipid sulfation, the response of progenitor cells to specific growth factors, and developmental lineage progression. The experimental system used is the oligodendrocyte (OL), the cell responsible for myelination of axons in the central nervous system. The ability to ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1105
更新日期：1994-04-01 00:00:00
abstract：:Canonical Wnt signalling is known to be involved in the regulation of differentiation and proliferation in the context of endodermal organogenesis. Wnt mediated beta-catenin activation is understood to be modulated by secreted Frizzled-related proteins, such as XsFRP5, which is dynamically expressed in the prospective...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.03.004
更新日期：2009-05-15 00:00:00
