Abstract:
:1,25-Dihydroxyvitamin D(3) (1,25D) regulates gene transcription through a nuclear vitamin D receptor (VDR) which acts as a ligand-regulated transcription factor. Some structural vitamin D analogs (VDAs) are selective in their biological actions, because they retain cell-differentiating potential, while their calcemic activity is reduced. In this article we have shown that in untreated HL60 cells the expression level of VDR is low, in spite of constant presence of VDR mRNA. Furthermore we have shown that one of the most rapid effects of either 1,25D or VDAs is nuclear accumulation of VDR, which is proportional to the differentiation-inducing potential of given analog. We observed this effect not only in HL60 cells, but also in blast cells isolated from patients with acute myeloid leukemias. After longer incubation time of the cells with various VDAs, the expression levels of VDR have become unrelated to the final differentiation effect.
journal_name
Steroidsjournal_title
Steroidsauthors
Gocek E,Kiełbiński M,Wyłób P,Kutner A,Marcinkowska Edoi
10.1016/j.steroids.2008.06.010subject
Has Abstractpub_date
2008-12-22 00:00:00pages
1359-66issue
14eissn
0039-128Xissn
1878-5867pii
S0039-128X(08)00191-8journal_volume
73pub_type
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