Abstract：
:The whole-cell nitrilase-catalyzed asymmetric hydrolysis of nitriles is a green and efficient preparation approach for chiral carboxylic acids, but often suffers from toxicity and cell lysis from organic substrates. In this work, a novel integrated process for whole-cell nitrilase-catalyzed asymmetric hydrolysis was developed for the first time by introducing a biocompatible ionic liquid (IL)-based biphasic system. The whole-cell nitrilases displayed an outstanding stability and recyclability in the biphasic system and still retained > 85% activity even after 7 cycles reaction. A preparative-scale fed-batch hydrolysis of o-chloromandelonitrile to (R)-o-chloromandelic acid (R-CMA) was performed using the integrated process. The results revealed a yield of 91.3% and a space-time yield of 746.4 g·L-1·d-1, which are currently the highest reported values for R-CMA biosynthesis. The proposed integrated process avoids substrate inhibition, facilitates the reusability of whole-cell nitrilases, and thus shows great potential for the sustainable production of chiral carboxylic acids.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zou S,Hua D,Jiang Z,Han X,Xue Y,Zheng Ydoi
10.1016/j.biortech.2020.124392subject
Has Abstractpub_date
2021-01-01 00:00:00pages
124392issue
Pt Beissn
0960-8524issn
1873-2976pii
S0960-8524(20)31666-7journal_volume
320pub_type
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