Abstract:
:A defined mixed moderately thermophilic consortium including three terrestrial microorganisms (Leptospirillum ferriphilum, Acidithiobacillus caldus and Ferroplasma thermophilum) and a marine acidophilic halotolerant bacterium (Sulfobacillus sp. TPY) was constructed to evaluate its ability for bioleaching of chalcopyrite with the addition of sodium chloride (NaCl), and the community dynamics was monitored by real-time quantitative PCR (qPCR). It was found that Sulfobacillus sp. TPY was able to tolerate 2% (w/v) NaCl, while other three microorganisms were suppressed when the concentration of NaCl was higher than 0.35%. The results suggested that NaCl below certain concentration could improve copper extraction by using pure cultures or the consortium to bioleach chalcopyrite. Community dynamics analysis during bioleaching at 0.1% NaCl showed that Sulfobacillus sp. TPY was predominant species during the whole bioleaching process, L. ferriphilum and A. caldus were less at any time compared with Sulfobacillus sp. TPY. F. thermophilum had never been dominant species even in the final stage.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Wang Y,Su L,Zhang L,Zeng W,Wu J,Wan L,Qiu G,Chen X,Zhou Hdoi
10.1016/j.biortech.2012.06.114subject
Has Abstractpub_date
2012-10-01 00:00:00pages
348-54eissn
0960-8524issn
1873-2976pii
S0960-8524(12)01029-2journal_volume
121pub_type
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