Metabolic engineering of Escherichia coli for 1,3-propanediol biosynthesis from glycerol.

Abstract:

:In this study, the engineered E. coli was constructed for efficient transformation of glycerol to 1,3-propanediol (1,3-PDO). To regenerate NADPH, the key bottleneck in 1,3-PDO production, heterologous NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDN, encoded by gapN) pathway was introduced, and the gapN expression level was fine-tuned with specific 5'-untranslated regions (5'-UTR) to balance the carbon flux distribution between the metabolic pathways of NADPH regeneration and 1,3-PDO biosynthesis. Additionally, glucose was added to the medium to promote glycerol utilization and cell growth. To elevate the utilization of glycerol in the presence of glucose, E. coli JA11 was constructed through destroying PEP-dependent glucose transport system while strengthening the ATP-dependent transport system. Subsequent optimization of nitrogen sources further improved 1,3-PDO production. Finally, under the optimal fermentation condition, E. coli JA11 produced 13.47 g/L 1,3-PDO, with a yield of 0.64 mol/mol, increased by 325% and 100% compared with the original engineered E. coli JA03, respectively.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Yang B,Liang S,Liu H,Liu J,Cui Z,Wen J

doi

10.1016/j.biortech.2018.07.082

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

599-607

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(18)31007-1

journal_volume

267

pub_type

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