Abstract:
:In this study, the engineered E. coli was constructed for efficient transformation of glycerol to 1,3-propanediol (1,3-PDO). To regenerate NADPH, the key bottleneck in 1,3-PDO production, heterologous NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDN, encoded by gapN) pathway was introduced, and the gapN expression level was fine-tuned with specific 5'-untranslated regions (5'-UTR) to balance the carbon flux distribution between the metabolic pathways of NADPH regeneration and 1,3-PDO biosynthesis. Additionally, glucose was added to the medium to promote glycerol utilization and cell growth. To elevate the utilization of glycerol in the presence of glucose, E. coli JA11 was constructed through destroying PEP-dependent glucose transport system while strengthening the ATP-dependent transport system. Subsequent optimization of nitrogen sources further improved 1,3-PDO production. Finally, under the optimal fermentation condition, E. coli JA11 produced 13.47 g/L 1,3-PDO, with a yield of 0.64 mol/mol, increased by 325% and 100% compared with the original engineered E. coli JA03, respectively.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Yang B,Liang S,Liu H,Liu J,Cui Z,Wen Jdoi
10.1016/j.biortech.2018.07.082subject
Has Abstractpub_date
2018-11-01 00:00:00pages
599-607eissn
0960-8524issn
1873-2976pii
S0960-8524(18)31007-1journal_volume
267pub_type
杂志文章abstract::The aim of this work was to examine the applicability of the anaerobic ammonium oxidation (anammox) process to three kinds of low BOD/N ratio wastewaters from animal waste treatment processes in batch mode. A rapid decrease of NO(2)(-) and NH(4)(+) was observed during incubation with wastewaters from AS and UASB/trick...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2006.09.031
更新日期:2007-10-01 00:00:00
abstract::Lignin valorisation into renewable fuels and platform chemicals is desirable but still encounters major challenges due to lignin's recalcitrant structure, and the lack of cost-, energy-, and material efficient conversion processes. Herein, we report a low-temperature plasma-based route to lignin depolymerisation at mi...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2020.123917
更新日期:2020-12-01 00:00:00
abstract::Graphiumputredinis, Trichodermaharzianum and fusant were used in the present study to produce extracellular xylanases, an important industrial enzyme used in pulp and paper industry produced in a minimal medium supplemented with oat spelt xylan (1%, w/v) pH 7.0 at 27+/-2 degrees C. The enzyme was purified to homogenei...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2008.07.014
更新日期:2009-01-01 00:00:00
abstract::Co-fermentation and simultaneous saccharification of rice hull hydrolysate (RHH) were investigated for the production of ethanol and xylitol by Saccharomyces cerevisiae, Spathaspora arborariae, or the combination of both. In bioreactor cultures under oxygen limitation, S. cerevisiae was capable of metabolizing glucose...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.05.123
更新日期:2013-09-01 00:00:00
abstract::A two-phase and three-phase predictive fluidization model based on the characteristics of a system such as media type and size, flow rates, and reactor cross sectional area was proposed to calculate bed expansion, solid, liquid and gas hold up and specific surface area (SSA) of the biofilm particles. The model was sub...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.10.110
更新日期:2011-02-01 00:00:00
abstract::In bioelectrochemical systems (BESs), electrons are transferred between electrochemically active microbes (EAMs) and conductive materials, such as electrodes, via extracellular electron transfer (EET) pathways, and electrons thus transferred stimulate intracellular catabolic reactions. Catabolic and EET pathways have ...
journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2018.01.125
更新日期:2018-05-01 00:00:00
abstract::Maintenance of stable pH during pyrite-oxidizing denitrification process is important. Here, we demonstrated effective pH control (7.80 ± 0.20-8.40 ± 0.30) in an electrochemical-H2 and pyrite-oxidizing denitrifying bioreactor (HPR) through in situ electrohydrogenesis. HPR achieved a higher nitrate removal activity (ma...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.02.069
更新日期:2019-06-01 00:00:00
abstract::Physicochemical evolution (i.e. pore structure variation, carbon structure change and active AAEM transformation) during rice straw (RS) and Shenfu bituminous coal (SF) co-pyrolysis was quantitatively determined in this work. Moreover, the corresponding char gasification was conducted using a thermogravimetric analyze...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.12.068
更新日期:2017-03-01 00:00:00
abstract::A simple technique, REMI (restriction enzyme-mediated integration), was used to construct transformants of Trichoderma atroviride with improved capability of degrading organophosphate pesticide dichlorvos. Linearized DNA of plasmid pV2 bearing the hygromycin B phosphotransferase (hph) gene was inserted into chromosome...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2008.05.022
更新日期:2009-01-01 00:00:00
abstract::In the present study, ethanol production from polysaccharides or wood chips was conducted in a single reactor under anaerobic conditions using the white rot fungus Schizophyllum commune NBRC 4928, which produces enzymes that degrade lignin, cellulose and hemicellulose. The ethanol yields produced from glucose and xylo...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2015.08.031
更新日期:2015-12-01 00:00:00
abstract::This study prepared a carboxylic functionalized bioadsorbent that met the "4-E" criteria: Efficient, Economical, Environmentally friendly, and Easily-produced. Sesame straw (Sesamum indicum L.) was functionalized through treatment with citric acid (SSCA) and tartaric acid (SSTA). The products were examined for adsorpt...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2017.04.066
更新日期:2017-08-01 00:00:00
abstract::Microbial community structures and performance of air-cathode microbial fuel cells (MFCs) inoculated with activated sludge from domestic wastewater were investigated to evaluate the effects of three substrate pre-acclimation strategies: 1, serial pre-acclimation with acetate and glucose before supplying domestic waste...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2017.02.101
更新日期:2017-06-01 00:00:00
abstract::To improve microalgae harvesting efficiency and to reduce the addition of chemicals in the buoy-bead flotation process, a novel buoy-bead flotation approach has been developed for harvesting Chlorella vulgaris, using surface-layered polymeric microspheres (SLPMs). Next, the detachment of microalgae cell-SLPM aggregate...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2018.07.065
更新日期:2018-11-01 00:00:00
abstract::Hydrogen can be produced by catalytic steam reforming (CSR) of biomass-derived oil. Typically bio oil contains 12-14% acetic acid; therefore, this acid was chosen as model compound for reforming of biooil with the help of a Cu-Zn/Ca-Al catalyst for high yield of H(2) with low CH(4) and CO content. Calcium aluminate su...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.07.019
更新日期:2012-11-01 00:00:00
abstract::Fe is widely used as an additive in anaerobic digestion, but its bioavailability and the mechanism by which it enhances digestion are unclear. In this study, sequential extraction was used to measure Fe bioavailability, while biochemical parameters, kinetics model and Q-PCR (fluorescence quantitative PCR) were used to...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2017.07.151
更新日期:2018-02-01 00:00:00
abstract::The potential of the adsorbent prepared from DOJR marketed as cattle food in Japan was identified as an efficient and low cost adsorbent for fluoride using fixed bed column. DOJR was loaded with Zr(IV) ions to develop active adsorption sites for fluoride. Fluoride adsorption performance of column packed with Zr(IV) lo...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.07.014
更新日期:2013-10-01 00:00:00
abstract::The anaerobic co-digestion of glycerol and wastewater derived from biodiesel manufacturing, in which COD was found to be 1054 and 428 g/L, respectively, was studied in batch laboratory-scale reactors at mesophilic temperature (35 degrees C). Glycerol was acidified with H(3)PO(4) in order to recover the alkaline cataly...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.03.042
更新日期:2010-08-01 00:00:00
abstract::The effects of hydrothermal conditions for pretreating wheat bran on the quality of residual protein for animal feeding, and on monosaccharide release for ethanol production were studied according to a 4×2×2 design with the factors, temperature (120, 140, 160, and 180°C), acidity (pH 2.3 and 3.9), and retention time (...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.08.052
更新日期:2012-11-01 00:00:00
abstract::The membrane fouling mechanism was studied in treating succinic acid fermentation broth during dead-end ultrafiltration. Different membranes were used and two models were applied to analyze the fouling mechanism. Resistance-in-series model was applied to determine the main factor that caused the operation resistance. ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.03.099
更新日期:2012-07-01 00:00:00
abstract::A wall-jet microbial fuel cell (MFC) was developed for the monitoring of anaerobic digestion (AD). This biofilm based MFC biosensor had a character of being portable, short hydraulic retention time (HRT) for sample flow through and convenient for continuous operation. The MFC was installed in the recirculation loop of...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.08.053
更新日期:2011-11-01 00:00:00
abstract::In this study, biochar derived from municipal sludge was activated by zinc chloride, which was first time used as the precursor for hydrothermal synthesis of magnetic sludge biochar (Fe/Zn-SBC) for tetracycline (TC) and ciprofloxacin (CIP) removal. The maximum adsorption capacity of Fe/Zn-SBC for TC and CIP were 145 m...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2020.124199
更新日期:2021-01-01 00:00:00
abstract::Nitrate-dependent anaerobic ferrous oxidizing (NAFO) is a valuable biological process, which utilizes ferrous iron to convert nitrate into nitrogen gas, removing nitrogen from wastewater. In this work, the performance of NAFO process was investigated as a nitrate removal technology. The results showed that NAFO system...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.12.036
更新日期:2015-03-01 00:00:00
abstract::The aims of this study were to assess changes in heavy metal availability in two contrasting feedstocks during aerobic composting, and the availability of said metals in the finished composts. A high C-to-N ratio mixed biodegradable municipal solid waste (MSW) feedstock was successfully composted on its own and in com...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2009.04.023
更新日期:2009-10-01 00:00:00
abstract::Sewage sludge rich in carbohydrates and other nutrients could be a good feedstock for fuel/chemical production. In this study, fungal and engineered bacterial cultivations were integrated with a modified anaerobic digestion to accumulate fatty acids on sewage sludge. The anaerobic digestion was first adjusted to enabl...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.01.017
更新日期:2013-03-01 00:00:00
abstract::The objective of this work was to examine the feasibility of biogas production from the anaerobic co-digestion of herbal-extraction residues with swine manure. Batch and semi-continuous experiments were carried out under mesophilic anaerobic conditions. Batch experiments revealed that the highest specific biogas yield...
journal_title:Bioresource technology
pub_type: 杂志文章,收录出版
doi:10.1016/j.biortech.2011.03.093
更新日期:2011-06-01 00:00:00
abstract::The aim of this work was to study biologically reduced graphene oxide (RGO) for engineering the surface architecture of the bioelectrodes to improve the performance of Bioelectrochemical System (BES). Gluconobacter roseus mediates the reduction of graphene oxide (GO). The RGO modified bioelectrodes produced a current ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2018.02.001
更新日期:2018-05-01 00:00:00
abstract::A novel integrated process with three-dimensional electro-Fenton (3D EF) and biological activated carbon (BAC) was employed in advanced treatment of biologically pretreated Lurgi coal gasification wastewater. SAC-Fe (sludge deserved activated carbon from sewage and iron sludge) and SAC (sludge deserved activated carbo...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2015.07.068
更新日期:2015-11-01 00:00:00
abstract::This study developed a new and cost-effective method for the remediation of marine sediments contaminated with PAHs. Fe3O4 particles were synthesized as the active component, supported on bamboo biochar (BB) to form a composite catalyst (Fe3O4-BB). The effects of critical parameters, including the initial pH, sodium p...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2017.08.204
更新日期:2017-12-01 00:00:00
abstract::This study demonstrates that increased NADPH production can improve biomass and ethanol production in cyanobacteria. We over-expressed the endogenous zwf gene, which encodes glucose-6-phosphate dehydrogenase of pentose phosphate pathway, in the model cyanobacterium Synechocystis sp. PCC 6803. zwf over-expression resul...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.02.056
更新日期:2016-08-01 00:00:00
abstract::Different alternative configurations and strategies for the simultaneous biological removal of organic matter and nutrients (N and P) in wastewater have been proposed in the literature. This work demonstrates a new successful strategy to bring in enhanced biological phosphorus removal (EBPR) to a conventional nitrific...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.05.041
更新日期:2011-09-01 00:00:00