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Improved degradation of organophosphate dichlorvos by Trichoderma atroviride transformants generated by restriction enzyme-mediated integration (REMI).

Abstract：

:A simple technique, REMI (restriction enzyme-mediated integration), was used to construct transformants of Trichoderma atroviride with improved capability of degrading organophosphate pesticide dichlorvos. Linearized DNA of plasmid pV2 bearing the hygromycin B phosphotransferase (hph) gene was inserted into chromosomes of wild strain T23 and transformation was confirmed by PCR and Southern blot analysis, respectively. Of 247 transformants, 76% showed improved dichlorvos degradation ability as compared to the parent strain T23 based on the least significant difference (LSD) test at p=0.01. Among them, 8 transformants exhibited 30% higher in degradation rate than the parent isolate. The highest dichlorvos degradation rate of the transformants was up to 96%. This study provided an effective approach for improving organophosphate pesticide-degrading capability of T. atroviride.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Tang J,Liu L,Hu S,Chen Y,Chen J

doi

10.1016/j.biortech.2008.05.022

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

480-3

issue

1

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(08)00452-5

journal_volume

100

pub_type

杂志文章

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