Molecular characterization of anaerobic digester microbial communities identifies microorganisms that correlate to reactor performance.

Abstract:

:A time-course analysis was conducted of thermophilic anaerobic digestion of dairy manure and wheat distillery thin stillage. Sequencing of chaperonin targets provided a phylogenetic survey of both bacteria and archaea in the digestate, along with an appraisal of the diversity of the reactor microbiome. A total of 1129 bacterial operational taxonomic units (OTU) were detected in the reactors, with OTU related to Clostridium becoming numerically dominant by day 7, and Acetivibrio-related OTU by day 35. Archaeal communities were less diverse, with 19 OTU detected representing both acetoclastic and hydrogenotrophic methanogens. Regardless of input material, the same organisms came to dominate the reactors, reflecting strong selective pressures present in the digesters. Principal coordinate analysis of the microbial communities showed that the bacterial communities clustered based on factors other than input material. Bacterial and archaeal OTU were identified with significant correlations to performance parameters, suggesting important roles in the methane production pathway.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Town JR,Links MG,Fonstad TA,Dumonceaux TJ

doi

10.1016/j.biortech.2013.10.070

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

249-57

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(13)01657-X

journal_volume

151

pub_type

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