Production and characterization of an intracellular bioflocculant by Chryseobacterium daeguense W6 cultured in low nutrition medium.

Abstract:

:A novel intracellular bioflocculant (named MBF-W6) produced by Chryseobacterium daeguense W6 cultured in low nutrition medium was investigated in this study. The effects of carbon source, nitrogen source, C/N ratio, initial pH, inoculum size, culture temperature and shaking speed on MBF-W6 production were studied. Chemical analysis showed that the purified MBF-W6 was mainly composed of 32.4% protein, 13.1% polysaccharide and 6.8% nucleic acid. Fourier-transform infrared (FTIR) spectroscopy indicated the presence of carboxyl, hydroxyl, and methoxyl groups. The elemental analysis of purified MBF-W6 revealed that the mass proportion of C, H, O, N and S was 40.92:6.53:44.01:8.53:1.01 (w/w) correspondingly. MBF-W6 had good flocculating rate in Kaolin suspension without any cation addition. The highest flocculating rate of 96.9% was achieved under the optimal conditions (bioflocculant dosage 1.2 mg l(-1), pH 5.6 and temperature 15 degrees C).

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Liu W,Wang K,Li B,Yuan H,Yang J

doi

10.1016/j.biortech.2009.08.108

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

1044-8

issue

3

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(09)01172-9

journal_volume

101

pub_type

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