Abstract：
:The laccase gene lac48424-1 and its corresponding full-length cDNA were cloned and characterized from a novel white-rot fungi Trametes sp. 48424 which had the high yield of laccase and strong ability for decolorizing different dyes. The 1563 bp full-length cDNA of lac48424-1 encoded a mature laccase protein containing 499 amino acids preceded by a signal peptide of 21 amino acids. The deduced protein sequence of LAC48424-1 showed high similarity with other known fungal laccases and contained four copper-binding conserved domains of typical laccase protein. The functionality of lac48424-1 gene encoding active laccase was verified by expressing the gene in the yeast Pichia pastoris successfully. It was found that the recombinant laccase produced by the yeast transformant could decolorize different dyes. The 5'-flanking sequence upstream of start codon was obtained by Self-Formed Adaptor PCR. Many putative cis-acting responsive elements involved in the transcriptional regulation were identified in the promoter region of lac48424-1.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Fan F,Zhuo R,Sun S,Wan X,Jiang M,Zhang X,Yang Ydoi
10.1016/j.biortech.2010.10.079subject
Has Abstractpub_date
2011-02-01 00:00:00pages
3126-37issue
3eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01735-9journal_volume
102pub_type
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