Abstract:
:In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20F88W-N119W gene resulted in further linalool production. A maximum linalool titre of 6.96±0.29mg/L was achieved in shake flasks, which was the highest level ever reported in yeasts.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Cao X,Wei LJ,Lin JY,Hua Qdoi
10.1016/j.biortech.2017.06.105subject
Has Abstractpub_date
2017-12-01 00:00:00pages
1641-1644issue
Pt Beissn
0960-8524issn
1873-2976pii
S0960-8524(17)31009-Xjournal_volume
245pub_type
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