Abstract：
:Jerusalem artichoke stalk and tuber can serve as a low cost feedstock for the production of 2,3-butanediol. However, like other lignocellulosic materials, the sugar concentration in the hydrolysate prepared from Jerusalem artichoke stalk is too low to be utilized effectively by microorganisms. In this paper a novel strategy was proposed to increase the sugar concentration by adding Jerusalem artichoke tuber into the hydrolysate of the stalk. The sugar was then biotransformed into high-valued 2,3-butanediol by Klebsiella pneumoniae. Fed-batch simultaneous saccharification and fermentation (SSF) was effectively performed and 901.2 mmol/l (80.5 g/l) target products (2,3-butanediol plus acetoin) was obtained in 68 h by a stage-shift aeration strategy. The concentration, yield and productivity of target products were 16.9%, 16.8% and 23.4%, respectively, higher than the best results obtained with SSF operated under constant aeration. This showed that adding tuber to the stalk hydrolysate was a useful strategy for increasing the production of 2,3-butanediol from Jerusalem artichoke via fermentation.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Li D,Dai JY,Xiu ZLdoi
10.1016/j.biortech.2010.06.041subject
Has Abstractpub_date
2010-11-01 00:00:00pages
8342-7issue
21eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01022-9journal_volume
101pub_type
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