Abstract:
:The microbial community in endogenous denitrification and denitrifying phosphorus removal treatment at transcription level was unknown. This study first confirmed the expression of actually active bacteria in endogenous denitrification and denitrifying phosphorus removal system to treat low C/N municipal wastewater. No external carbon source was added to influent wastewater. The cDNA high throughput sequencing showed that Candidatus Accumulibacter was the most effective polyphosphate accumulating organisms (PAOs) that actually worked rather than Dechloromonas, which was different from the result at gene level. Reverse transcriptional PCR (RT-PCR) and analysis of Variance (ANOVA) suggested that the ratios of dead or dormant bacteria could monitor wastewater treatment process. Identification of active microbial community at transcription level demonstrated that the synergy of endogenous denitrification by glycogen accumulating organisms (GAOs) and denitrifying phosphorus removal by Candidatus Accumulibacter fully utilized the internal carbon source, and effectively solved the problem of carbon source deficiency in municipal wastewater treatment.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Fan Z,Zeng W,Wang B,Guo Y,Meng Q,Peng Ydoi
10.1016/j.biortech.2019.122454subject
Has Abstractpub_date
2020-02-01 00:00:00pages
122454eissn
0960-8524issn
1873-2976pii
S0960-8524(19)31684-0journal_volume
297pub_type
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