Abstract：
:Simultaneous production of citric acid (CA) and invertase by Yarrowia lipolytica A-101-B56-5 (SUC(+) clone) growing from sucrose, mixture of glucose and fructose, glucose or glycerol was investigated. Among the tested substrates the highest concentration of CA was reached from glycerol (57.15 g/L) with high yield (Y(CA/S)=0.6g/g). When sucrose was used, comparable amount of CA was secreted (45 g/L) with slightly higher yield (Y(CA/S)=0.643 g/g). In all cultures amount of isocitrate (ICA) was below 2% of total citrates. Considering invertase production, the best carbon source appeared to be sucrose (72380 U/L). The highest yield of CA and invertase biosynthesis calculated for 1g of biomass was obtained for cells growing from glycerol (9.9 g/g and 4325 U/g, respectively). Concentrates of extra- and intracellular invertase of the highest activity were obtained from sucrose as substrate (0.5 and 1.8 × 10(6)U/L, respectively).
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Lazar Z,Walczak E,Robak Mdoi
10.1016/j.biortech.2011.04.032subject
Has Abstractpub_date
2011-07-01 00:00:00pages
6982-9issue
13eissn
0960-8524issn
1873-2976pii
S0960-8524(11)00526-8journal_volume
102pub_type
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