Optimization of the hydrogen yield from single-stage photofermentation of glucose by Rhodobacter capsulatus JP91 using response surface methodology.

Abstract:

:Hydrogen production from glucose via single-stage photofermentation was examined with the photosynthetic bacterium Rhodobacter capsulatus JP91 (hup-). Response surface methodology with Box-Behnken design was used to optimize the independent experimental variables of glucose concentration, glutamate concentration and light intensity, as well as examining their interactive effects for maximization of molar hydrogen yield. Under optimal condition with a light intensity of 175W/m(2), 35mM glucose, and 4.5mM glutamate, a maximum hydrogen yield of 5.5 (±0.15)molH(2)/molglucose, and a maximum nitrogenase activity of 246 (±3.5)nmolC(2)H(4)/ml/min were obtained. Densitometric analysis of nitrogenase Fe-protein expression under different conditions showed significant variation in Fe-protein expression with a maximum at the optimized central point. Even under optimum conditions for hydrogen production, a significant fraction of the Fe-protein was found in the ADP-ribosylated state, suggesting that further improvement in yields might be possible.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Ghosh D,Sobro IF,Hallenbeck PC

doi

10.1016/j.biortech.2012.07.061

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

199-206

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)01108-X

journal_volume

123

pub_type

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