Abstract：
:A strain Bacillus sp. GZT capable of debrominating and mineralizing 2,4,6-tribromophenol (TBP) was isolated and characterized by morphological observation, biochemical and physiological identification as well as 16S rRNA sequence analysis. Biodegradation kinetics experiments demonstrated that initial TBP concentration had a predominant effect on degradation efficiency. Within 120h, the highest TBP degradation and debromination efficiencies were up to 93.2% and 89.3%, respectively, under the optimum condition. Ten metabolic intermediates including five brominated compounds, three oxidative products and two cellular metabolites were all identified by gas chromatography-mass spectrometer, and six key intermediates were doubly validated by authentic standards. The proposed biodegradation mechanism inferred that reductive debromination as a major degradation pathway could simultaneously take place at ortho- and para-positions on TBP, while methylated debromination was also found as a minor degradation pathway during this process. Within 148h degradation, nearly one-third of 3mg/L TBP could be completely mineralized.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zu L,Li G,An T,Wong PKdoi
10.1016/j.biortech.2012.01.131subject
Has Abstractpub_date
2012-04-01 00:00:00pages
153-9eissn
0960-8524issn
1873-2976pii
S0960-8524(12)00155-1journal_volume
110pub_type
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