Abstract：
:The use of acetate as carbon feedstock can enhance sustainability and economics of the current bio-productions. This study explored the potential of acetate for the production of 3-hydroxypropionic acid by engineered Pseudomonas denitrificans. Heterologous mcr (encoding malonyl-CoA reductase) from Chloroflexus aurantiacus and endogenous accABCD (encoding acetyl-CoA carboxylase) were overexpressed in P. denitrificans. Carbon flux to 3-HP synthesis at the malonyl-CoA node was promoted by suppressing fatty acid synthesis through addition of cerulenin or deletion of fabF gene. In addition, stimulation of glyoxylate shunt and/or TCA cycle were attempted. Recombinant P. denitrificans overexpressing mcr and accABCD produced 19.3 mM 3-HP with cerulenin addition, and 14.2 mM with fabF deletion, respectively. Furthermore, the non-growing cells devoid of fabF could continuously produce 3-HP up to 40.4 mM without losing its production activity for 22 h. This study demonstrates that acetate is a good substrate for 3-HP production by recombinant P. denitrificans.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhou S,Lama S,Jiang J,Sankaranarayanan M,Park Sdoi
10.1016/j.biortech.2020.123194subject
Has Abstractpub_date
2020-07-01 00:00:00pages
123194eissn
0960-8524issn
1873-2976pii
S0960-8524(20)30465-Xjournal_volume
307pub_type
杂志文章abstract：:Rice straw was used as a carbon source in a denitrifying bioreactor, for the removal of nutrients from agricultural drainage. Nutrient removal efficiency was evaluated by: (a) nutrient loading rates (low, medium, and high); (b) hydraulic retention time, and (c) comparison with another carbon source (woodchip). The res...
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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