Abstract:
:Klebsiella oxytoca strains were constructed to produce optical pure d-lactate by pH-controlled batch fermentation in mineral salts medium. The alcohol dehydrogenase gene, adhE, and the phospho-transacetylase/acetate kinase A genes, pta-ackA, were deleted from the wild type. KMS002 (ΔadhE) and KMS004 (ΔadhE Δpta-ackA) exhibited d-lactate production as a primary pathway for the regeneration of NAD(+). Both strains produced 11-13 g/L of d-lactate in medium containing 2% (w/v) glucose with yields of 0.64-0.71 g/g glucose used. In sugarcane molasses, KMS002 and KMS004 produced 22-24 g/L of d-lactate with yields of 0.80-0.87 g/g total sugars utilized. Both strains also utilized maltodextrin derived from cassava starch and produced d-lactate at a concentration of 33-34 g/L with yields of 0.91-0.92 g/g maltodextrin utilized. These d-lactate yields are higher than those reported for engineered E. coli strains.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Sangproo M,Polyiam P,Jantama SS,Kanchanatawee S,Jantama Kdoi
10.1016/j.biortech.2012.05.114subject
Has Abstractpub_date
2012-09-01 00:00:00pages
191-8eissn
0960-8524issn
1873-2976pii
S0960-8524(12)00871-1journal_volume
119pub_type
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