Abstract：
:This study demonstrated the effective application of intracellular azoreductase in mediated decolorization of azo dyes. Using the quinone reductase activity of overexpressed azoreductase AZR and quinone redox mediators, the decolorization performance of the recombinant strain Escherichia coli YB was significantly enhanced. In the presence of 0.2 mM lawsone, 75% acid red 27 (1 mM) was decolorized by E. coli YB in only 2 h, which was the highest bacterial decolorization rate ever reported. Compared to lawsone, menadione was a less effective redox mediator. Glucose was found to be the best carbon source for mediated decolorization by E. coli YB. The recombinant strain could complete four rounds of mediated decolorization repeatedly in 12 h. In addition, a 10-min pre-incubation of E. coli JM109 and activated sludge with 2-methylhydroquinone resulted in great improvement of mediated decolorization performance, which may be applied in practical treatment.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Liu G,Zhou J,Wang J,Zhou M,Lu H,Jin Rdoi
10.1016/j.biortech.2008.12.040subject
Has Abstractpub_date
2009-06-01 00:00:00pages
2791-5issue
11eissn
0960-8524issn
1873-2976pii
S0960-8524(08)01099-7journal_volume
100pub_type
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