Abstract：
:The influence of cellulose accessibility and protein loading on the efficiency of enzymatic hydrolysis of steam pretreated Douglas-fir was assessed. It was apparent that the lignin component significantly influences the swelling/accessibility of cellulose as at low protein loadings (5FPU/g cellulose), only 16% of the cellulose present in the steam pretreated softwood was hydrolyzed while almost complete hydrolysis was achieved with the delignified substrate. When lignin (isolated from steam pretreated Douglas-fir) was added back in the same proportions it was originally found to the highly accessible and swollen, delignified steam pretreated softwood and to a cellulose control such as Avicel, the hydrolysis yields decreased by 9 and 46%, respectively. However, when higher enzyme loadings were employed, the greater availability of the enzyme could overcome the limitations imposed by both the lignin's restrictions on cellulose accessibility and direct binding of the enzymes, resulting in a near complete hydrolysis of the cellulose.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kumar L,Arantes V,Chandra R,Saddler Jdoi
10.1016/j.biortech.2011.09.091subject
Has Abstractpub_date
2012-01-01 00:00:00pages
201-8issue
1eissn
0960-8524issn
1873-2976pii
S0960-8524(11)01379-4journal_volume
103pub_type
杂志文章abstract：:In this investigation, Enteromorpha compressa algal oil with high free fatty acids (FFA) used as a feedstock for biodiesel production. Two step process was developed and kinetic study executed to obtain reaction rate constant for the transesterification reaction. The acid esterification was carried out to reduce FFA f...
journal_title：Bioresource technology
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pub_type： 杂志文章,多中心研究
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更新日期：2005-01-01 00:00:00
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