Abstract:
:In this study, Lysinibacillus sp. RGS was evaluated to synthesize polyhydroxybutyrate (PHB) from a broad range of pure carbon sources and residual sugars of chemically pretreated sugarcane bagasse (SCB) hydrolysates. Effects of supplementation of nutrients and various experimental variables to enhance PHB accumulation were investigated. Results of optimized parameters were identified as 48 h, 37 °C, pH 7; inoculums concentration (2.5% v/v) and shaking condition (100 rpm). Growth kinetics and bioprocess parameters of Lysinibacillus sp. using SCB hydrolysates with corn steep liquor (2%) accounted for the maximum cell growth (8.65 g/L) and PHA accumulation (61.5%) with PHB titer of (5.31 g/L) under optimal conditions. The produced biopolymer was studied by Fourier Transform Infrared (FTIR) spectroscopy and the results revealed the obtained to be PHB. Thus Lysinibaciluus sp. exhibits high potential in industrial scale manufacture of PHB using SCB as an inexpensive substrate.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Saratale RG,Cho SK,Saratale GD,Ghodake GS,Bharagava RN,Kim DS,Nair S,Shin HSdoi
10.1016/j.biortech.2021.124673subject
Has Abstractpub_date
2021-03-01 00:00:00pages
124673eissn
0960-8524issn
1873-2976pii
S0960-8524(21)00011-0journal_volume
324pub_type
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