Abstract：
:Rapeseed meal (RSM) hydrolysate was evaluated as substitute for commercial nutrient supplements in 1,3-propanediol (PDO) fermentation using the strain Clostridium butyricum VPI 1718. RSM was enzymatically converted into a generic fermentation feedstock, enriched in amino acids, peptides and various micro-nutrients, using crude enzyme consortia produced via solid state fermentation by a fungal strain of Aspergillus oryzae. Initial free amino nitrogen concentration influenced PDO production in batch cultures. RSM hydrolysates were compared with commercial nutrient supplements regarding PDO production in fed-batch cultures carried out in a bench-scale bioreactor. The utilization of RSM hydrolysates in repeated batch cultivation resulted in a PDO concentration of 65.5 g/L with an overall productivity of 1.15 g/L/h that was almost 2 times higher than the productivity achieved when yeast extract was used as nutrient supplement.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Chatzifragkou A,Papanikolaou S,Kopsahelis N,Kachrimanidou V,Dorado MP,Koutinas AAdoi
10.1016/j.biortech.2014.02.021subject
Has Abstractpub_date
2014-05-01 00:00:00pages
167-75eissn
0960-8524issn
1873-2976pii
S0960-8524(14)00192-8journal_volume
159pub_type
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