Abstract：
:Biodegradation of ferulic acid, by an endophytic fungus called Phomopsis liquidambari was investigated in this study. This strain can use ferulic acid as the sole carbon for growth. Both in mineral salt medium and in soil, more than 97% of added ferulic acid was degraded within 48 h. The metabolites were identified and quantified using GC-MS and HPLC-MS. Ferulic acid was first decarboxylated to 4-vinyl guaiacol and then oxidized to vanillin and vanillic acid, followed by demethylation to protocatechuic acid, which was further degraded through the β-ketoadipate pathway. During degradation, ferulic acid decarboxylase, laccase and protocatechuate 3,4-dioxygenase activities and their gene transcription levels were significantly affected by the variation of substrate and product concentrations. Moreover, ferulic acid degradation was determined to some extent by P. liquidambari laccase. This study is the first report of an endophytic fungus that has a great potential for practical application in ferulic acid-contaminated environments.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Xie XG,Dai CCdoi
10.1016/j.biortech.2014.11.112subject
Has Abstractpub_date
2015-03-01 00:00:00pages
35-42eissn
0960-8524issn
1873-2976pii
S0960-8524(14)01733-7journal_volume
179pub_type
杂志文章abstract：:Clostridium saccharoperbutylacetonicum N1-4 is well known as a hyper-butanol producing strain. However, little information is available concerning its butanol production mechanism and the development of more robust strains. In this study, key biosynthetic genes (either endogenous or exogenous) including the sol operon...
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pub_type： 杂志文章,评审
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更新日期：2016-09-01 00:00:00
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doi：10.1016/j.biortech.2013.10.051
更新日期：2014-01-01 00:00:00
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更新日期：2016-12-01 00:00:00
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